Modulation of NK and K Cell Activity by Trypsin Treatment of Effector Cells

Scheiner, Otto; Wiedermann, G; Rumpold, Helmut; Steiner, Rebekah; D, Kraft; Stemberger, H

doi:10.1016/s0171-2985(80)80004-0

Cited by 5 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amoebae's cytotoxic activity test (ACA) A modified microcytotoxicity assay was performed as described for K and NK lymphocytes 26 . K 562 target cells were labelled with 5iCr.…”

Section: Amoebae's Lysis Test (Al)mentioning

confidence: 99%

Interaction of rheumatoid factor and Entamoeba histolytica

Kremsner¹,

Graninger

1989

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYThe amoebae's cytotoxicity test and the amoebae's lysis test were used to show possible interactions between rheumatoid factor (RF) and Entamoeba histolytica. Amoebae's cytotoxic activity (ACA) was inhibited by affinity chromatography purified antiamoebae rabbit IgG (RIgG). Enhanced inhibition could be demonstrated with RIgG plus RF. But the same marked inhibition of ACA could be seen when replacing RF by heat inactivated normal human serum as a control.About 50% amoebae's lysis occurred when amoebae were brought together with native normal human serum (NNHS) as a source of complement. Amoebae's lysis increased to 60% when incubated with NHS plus human antiamoebae antibodies. No further augmentation could be obtained by the addition of RF. Using RIgG instead of human antibodies the lysis rate did not increase. Incubation of amoebae, NNHS, RIgG and RF even reduced amoebae's lysis. RF neither has an effect on ACA nor on complement mediated AL in vitro.

show abstract

“…Amoebae's cytotoxic activity test (ACA) A modified microcytotoxicity assay was performed as described for K and NK lymphocytes 26 . K 562 target cells were labelled with 5iCr.…”

Section: Amoebae's Lysis Test (Al)mentioning

confidence: 99%

Interaction of rheumatoid factor and Entamoeba histolytica

Kremsner¹,

Graninger

1989

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

Variance in resistance to natural and antibody‐dependent cellular cytotoxicity and to complement‐mediated lysis among K562 lines

Kimber

Moore

Roberts

1983

Intl Journal of Cancer

View full text Add to dashboard Cite

Isolation of sub-populations of the NK-sensitive erythro-leukaemic cell line K562 by limiting dilution techniques has revealed marked clonal variation in susceptibility to natural cytotoxicity. Detailed examination of two such lines (E10/P2 and F9/P2) which differed significantly in their susceptibility to both native and activated NK-cell-mediated lysis revealed that their differences were stable and independent of culture conditions. The resistant (F9/P2) and sensitive (E10/P2) lines had comparable cold-inhibitory and effector-cell adsorption capacities, indicating that differential susceptibility was not attributable to variable expression of NK target structures. F9/P2 was also less susceptible to antibody-dependent cellular cytotoxicity (ADCC) and complement-mediated lysis, indicating the existence of variants with a generalized capacity to resist several immunolytic processes.

show abstract

Cytotoxicity of human peripheral blood T-lymphocyte clones activated by hepatitis B virus surface antigen

Hakim

1985

Experientia

View full text Add to dashboard Cite

The present studies examined the cytotoxic activities of peripheral blood lymphocytes (PBL) from volunteers with (sero-positive) and without (sero-negative) circulating antibodies to hepatitis B virus surface antigen before and 30 days after vaccination with hepatitis B virus surface antigen (HBsAg). Long-term culture of monospecific hepatitis B surface (HBsAg)-responsive T-lymphocytes were isolated and grown in large numbers. The mechanism of T-cell mediated cytolysis, and the identification of the carbohydrate determinants on the surface of these effector cells responsible for the killing effect, are being examined.

show abstract

Modulation of NK and K Cell Activity by Trypsin Treatment of Effector Cells

Cited by 5 publications

References 21 publications

Interaction of rheumatoid factor and Entamoeba histolytica

Interaction of rheumatoid factor and Entamoeba histolytica

Variance in resistance to natural and antibody‐dependent cellular cytotoxicity and to complement‐mediated lysis among K562 lines

Cytotoxicity of human peripheral blood T-lymphocyte clones activated by hepatitis B virus surface antigen

Contact Info

Product

Resources

About