Cytotoxicity of human peripheral blood T-lymphocyte clones activated by hepatitis B virus surface antigen

Hakim, Anwar A.

doi:10.1007/bf01964817

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…The B-cell suppression may result from the fact that helper T-cells become non-responsive to a wide variety of stimuli, including antigenic as well as mitogenic stimulation [123]. There is strong evidence implicating oligosaccharide moieties of TA glycoproteins in the escape mechanism of tumor cells [124,125].…”

Section: Biofunctions Of Carbohydrate Molecules In Carcinomamentioning

confidence: 99%

The structural relationship of blood group-related oligosaccharides in human carcinoma to biological function: A perspective

Dube

1987

Cancer Metast Rev

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Blood group-related oligosaccharides have been isolated from a limited number of carcinomas. The carcinoma-associated oligosaccharides show chain elongation, for example due to repeating Gal 1,4 GlcNAc 1,3 sequences, or a higher degree of branching, which permit increased sialylation and fucosylation. Abnormal carbohydrate structures have been demonstrated on tumor cell membranes by immunological techniques, which suggests deletion of ABH, accumulation of 'crypt' antigens such as I and T antigens, and abnormal expression of Lewis antigens. Changes in carcinoma-associated oligosaccharides can result from altered biosynthetic processing in the Golgi apparatus or the occurrence of abnormal tumor glycosyltransferase isoenzymes. Structural alterations of oligosaccharides on the tumor cell membrane are related to the regulation of tumor growth, cell-cell interaction, cell differentiation, and metastasis. Glycoproteins secreted by tumor cells into the circulation evoke cellular and humoral immunity and cause immune suppression by binding to cytotoxic T lymphocytes and lymphocyte subsets. The relationship of oligosaccharide structures to biologic function awaits elucidation.

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Section: Biofunctions Of Carbohydrate Molecules In Carcinomamentioning

confidence: 99%

The structural relationship of blood group-related oligosaccharides in human carcinoma to biological function: A perspective

Dube

1987

Cancer Metast Rev

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Peripheral blood lymphocytes from patients with cancer lack interleukin-2 receptors

Hakim

1988

Cancer

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When tumor cells develop in healthy adults, they activate the cellular immune system--natural killer (NK) cells, antigen-specific cytotoxic lymphocytes (CTL), and the synthesis of antigen specific cytotoxic antibodies. These are aimed at killing the intruding cells. However, in cancer patients the tumor continues to grow. As tumor cells proliferate, they were shown to release factors that mediate the inactivation of the host immune defense systems. The study documented in this article examined peripheral blood lymphocytes, mononuclear cells (MNC), NK cells, T-helper cells (THC). This study confirmed the interaction of the released inhibitor factors with these mononuclear cells. NULL cells from healthy adults responding to interleukin-2 (IL-2) and NILL cells from patients with metastatic breast carcinoma nonresponsive to IL-2 were also isolated by the standard antibodies-pinning technique. The cells were obtained from age-matched subjects: ten healthy adults; ten patients each from Stage I, II, III, and IV metastatic breast carcinoma (BCa-I, BCa-II, BCa-III, and BCa-IV or MBCa); and ten patients with benign breast disease (BBD). The responsiveness of these THC, PBMNC, NK, NULL, and NILL cells in vitro to graded levels of phytohemagglutinin (PHA), Concanavalin A (Con A), and recombinant interleukin-2 (rIL-2) was examined. Responsiveness was monitored by 3H-thymidine (3H-TdR) uptake, production and release of IL-2, interleukin-2 receptor (IL-2R), and cytotoxic activities against K-562 cells and breast carcinoma short-term cell lines. A lack of functional IL-2R in peripheral blood lymphocytes from patients with metastatic breast carcinoma was confirmed by nonsignificant anti-Tac antibody binding. An elevation in the expression of cell surface antigen GP-120 has been observed to be associated with the activation in vitro of T-cells from healthy adults and from patients with benign breast disease, but not of T-cells from patients with breast carcinoma. Biochemical studies of the GP-120 using high performance liquid chromatography combined with nitrocellulose blotting confirmed that the glycoprotein was resistant to trypsin and chymotrypsin, but susceptible to pronase. It contained sialic acid and lactosaminoglycan as O-linked sugars. It could be labeled with pariodate/NaB(3H4) and is recognized by MAbT-305 monoclonal antibodies. It contained sialic acid linked (2---3) to galactose.(ABSTRACT TRUNCATED AT 400 WORDS)

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Cellular transfection with mRNA -encoding interleukin-2 receptor modulate human lymphocyte responsiveness

Hakim

1987

Naturwissenschaften

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Cytotoxicity of human peripheral blood T-lymphocyte clones activated by hepatitis B virus surface antigen

Cited by 3 publications

References 31 publications

The structural relationship of blood group-related oligosaccharides in human carcinoma to biological function: A perspective

The structural relationship of blood group-related oligosaccharides in human carcinoma to biological function: A perspective

Peripheral blood lymphocytes from patients with cancer lack interleukin-2 receptors

Cellular transfection with mRNA -encoding interleukin-2 receptor modulate human lymphocyte responsiveness

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