Modulation of myometrium mitochondrial membrane potential by calmodulin antagonists

Шлыков, С. Г.; Babich, L. G.; Yevtushenko, M E; Karakhim, S. O.; Костерін, С. О.

doi:10.15407/ubj86.01.029

Cited by 8 publications

(8 citation statements)

References 13 publications

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“…The difference between the relative values of mitochondrial membrane potential under oxidation of pyruvate and α‐ketoglutarate was 65.4% (Figure C). Such dependence of mitochondrial membrane polarization on oxidation substrates is consistent with the view that mitochondrial membrane potential and respiration rate are the highest with succinate oxidation …”

Section: Resultssupporting

confidence: 88%

“…Such dependence of mitochondrial membrane polarization on oxidation substrates is consistent with the view that mitochondrial membrane potential and respiration rate are the highest with succinate oxidation. 17,18 Ryanodine at 0.05-to 1-μM concentrations had no effects on the mitochondrial membrane potential under succinate and α-ketoglutarate oxidation after 3 minutes of incubation ( Figure 4A,C).…”

Section: Effect Of Ryanodine On Membrane Potential Of Rat Liver Mitmentioning

confidence: 98%

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Mitochondrial ryanodine‐sensitive Ca²⁺ channels of rat liver

Kupynyak

Ikkert

Шлыков

et al. 2017

Cell Biochemistry & Function

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To examine ryanodine-sensitive Ca channels in mitochondria of rat hepatocytes and their role in energy state of the cells via investigation of the ryanodine effect on mitochondrial membrane potential. Oxygen consumption was measured by polarography using the Clark electrode. The substrates of oxidation such as pyruvate (5mM), α-ketoglutarate (5mM), or succinate (5mM) were used. Oxidative phosphorylation was stimulated by the addition of adenosine diphosphate (200nM). Mitochondrial membrane potential was measured using a voltage-sensitive fluorescent probe tetramethylrhodamine-methyl-ester (0.1μM) and was analyzed by a flow cytometer. To evaluate the intact mitochondria, we used carbonil cyanide m-chlorophenyl hydrazone (CCCP, 10μM). Changes in the ionized calcium concentration in rat liver mitochondria were measured using a fluorescent probe Fluo-4 AM. Effect of ryanodine on oxygen consumption of rat liver mitochondria depends on the oxidation substrate and the incubation time. Oxidation of pyruvate in the presence of ryanodine (0.05μM) decreased the membrane potential of rat liver mitochondria by 38.4%. At higher concentrations, ryanodine (0.1μM or 1μM) led to decrease of membrane potential by 51.7% and 42.8%, respectively. In contrast, oxidation of α-ketoglutarate in the presence of ryanodine (0.05μM) increased mitochondrial membrane potential by 16.8%. However, at higher concentrations, ryanodine (0.1μM or 1μM) triggered a decreasing of membrane potential by 42.5% and 31.0%, respectively. Therefore, ryanodine at various concentrations (0.05μM, 0.1μM, or 1μM) causes differential effects on Ca concentration in the mitochondria matrix under oxidation of pyruvate or α-ketoglutarate. The data suggest the presence of ryanodine receptors in mitochondrial membrane of rat hepatocytes. Their inhibition with higher concentrations of ryanodine leads to decreasing of intra-mitochondrial Ca concentration and affecting the energy state of mictochondria in hepatocytes.

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Section: Resultssupporting

confidence: 88%

Section: Effect Of Ryanodine On Membrane Potential Of Rat Liver Mitmentioning

confidence: 98%

Mitochondrial ryanodine‐sensitive Ca²⁺ channels of rat liver

Kupynyak

Ikkert

Шлыков

et al. 2017

Cell Biochemistry & Function

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“…previously, conditions for attaching of myocytes to the flow chamber for confocal microscopy were developed [7]. Attached to the chamber surface myocytes were loaded with Ca 2+ -sensitive probe: 1 µM Fluo4 AM (λ exc .…”

Section: Methodsmentioning

confidence: 99%

“…Calmodulin-dependent regulation of Ca-ions metabolism in the myometrium mitochondria has been little studied. In a previous work, we had shown that the incubation of myometrium mitochondria with calmodulin antagonists such as calmidazolium (10 µM) or trifluope razine (100 µM) caused a mitochondria membrane depolarization [6,7]. The aim of this work was to investigate the effect of calmodulin antagonists on the ionized calcium level in the mitochondria and in the cytoplasm of uterine smooth muscle cells by using spectrofluorometry and confocal microscopy.…”

Section: +mentioning

confidence: 99%

Calmodulin antagonists effect on Ca(2+) level in the mitochondria and cytoplasm of myometrium cells

Шлыков¹,

Babich²,

Yevtushenko³

et al. 2015

Ukr.Biochem.J.

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“…Для візуалізації дії антагоністів кальмодуліну на мембранний потенціал мітохондрій клітин міометрія ми використали конфокальний мікроскоп та потенціалчутливі флуоресцентні зонди -MTG та TMRM. Показано, що інкубація клітин міометрія протягом 10 хв з 10 мкМ кальмідазоліумом або зі 100 мкМ трифлуопе разином (рисунок) веде до істотного знижен ня інтенсивності флуоресценції TMRM, що свідчить про деполяризуючий ефект цих сполук [73]. Рівень поляризації мембран мітохондрій та обмін іонів Са в них − ключові параметри, які відображають функціональний стан цих субклітинних структур у нормі та за патології [74,75].…”

Section: роль мітохондрій в са 2+ гомеостазіunclassified