Modulation of IL-8 mRNA expression and production in respiratory syncytial virus (RSV) stimulated human alveolar epithelial cells by budesonide and montelukast*1

Horne, N.S.; Shaw, HL; Huang, Yu‐Ching; Frieri, Marianne

doi:10.1016/j.jaci.2004.01.132

Cited by 4 publications

(2 citation statements)

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“…It is secreted by a wide range of cells including macrophages, monocytes, and epithelial and endothelial cells ( 14 , 15 ). Numbers of CXCL8 transcripts increase dramatically after stimulation by bacteria ( 16 – 18 ), virus ( 19 , 20 ), and certain cytokines such as IL-1 and tumor necrosis factor (TNF) ( 21 ). Structures of CXCL8 have been determined species across vertebrate groups, including human ( Homo sapiens ) ( 22 ), chicken ( Gallus gallus ) ( 23 ), frog ( Xenopus laevis ) ( 24 ), zebrafish ( Danio rerio ) ( 25 ), and river lamprey ( Lampetra fluviatilis ) ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Chemotactic Function of CXCL8 in Northeast Chinese Lamprey (Lethenteron morii)

et al. 2020

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Chemokine-induced chemotaxis of leukocytes is an important part of the innate immunity and has been shown to mediate inflammation in all groups of jawed vertebrates. For jawless vertebrates, hagfish leukocytes are known to show chemotaxis toward mammalian complement anaphylotoxin and Gram-negative bacteria lipopolysaccharide. However, whether chemokines mediate chemotaxis of leukocytes in jawless vertebrates has not been conclusively examined. Here, we show C-X-C motif chemokine ligand 8 (CXCL8, also named interleukin 8) of the Northeast Chinese lamprey (Lethenteron morii) (designated as LmCXCL8) induces chemotaxis in its leukocytes. We identified LmCXCL8 and found it possesses the characteristic N-terminal cysteine residues and GGR (Gly-Gly-Arg) motif. The Lmcxcl8 gene was found to be expressed in all examined tissues, and its expression was inducible in the lamprey challenged by an infectious bacterium, Pseudomonas aeruginosa. A recombinant LmCXCL8 protein elicited concentration-dependent chemotaxis in peripheral blood leukocytes isolated from the Northeast Chinese lamprey. Based on these results, we conclude that LmCXCL8 is a constitutive and inducible acute-phase cytokine that mediates immune defense and trace the chemotactic function of chemokine to basal vertebrates.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Chemotactic Function of CXCL8 in Northeast Chinese Lamprey (Lethenteron morii)

et al. 2020

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“…26 Additionally, A549 secreted increased amounts of soluble inflammatory mediators that were modulated by dust-mite extracts and drugs. [27][28][29][30] Other investigators showed that A549 cells are capable of secreting VEGF 31 that appears to be inducible 32 and can be inhibited by budesonide. 33 VEGF also may be cell-associated with airway epithelial cells as shown by electron microscopy.…”

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confidence: 99%

Vascular endothelial growth factor is increased by human pulmonary cells stimulated with Dermatophagoides sp. extract

Capetandes

Zhuang²,

Haque³

et al. 2007

allergy asthma proc

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Airway remodeling may lead to increased secretion of TGF-beta. TGF-beta is known to activate fibroblasts and to stimulate the secretion of vascular endothelial growth factor (VEGF). Soluble and cell-associated VEGF have been identified in subjects with chronic severe asthma. Dermatophagoides sp. has been shown to contribute to the pathogenesis of asthma. The aim of this study was to determine if the Dermatophagoides sp. extract can stimulate confluent A549 (cA549) to express soluble or cell-associated VEGF and to secrete other factors that stimulate normal human lung fibroblasts (NHLFs) to secrete VEGF. Immunocytochemistry for cell-associated VEGF (pg/mL) was performed on cA549 stimulated with 300, 600, and 1000 AU/mL of dialyzed Dermatophagoides sp. extract for 24 hours with serum-free media (SFM). The subsequent conditioned media (CM) was transferred to NHLF for 24 hours (CM-NHLF). VEGF in CM, CM-NHLF, and control media (CTLM; extract without cA549) were measured by ELISA and normalized to cell density as measured by absorbance of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide at A550 nm (VEGF pg/mL:A550). Parametric data were analyzed by t-test or ANOVA at alpha = 0.05. Dermatophagoides sp. extract increased normalized VEGF secretion by cA549. Immunochemical VEGF expression by cA549 was increased qualitatively by Dermatophagoides sp. extract. The 1000 CM-NHLF showed increased normalized VEGF relative to CTLM. Dermatophagoides sp. stimulates cA549 to increase soluble and immunochemical VEGF and to secrete mediators that stimulate NHLF to increase secretion of VEGF. This suggests that inhalation of dust-mite allergen may stimulate airway cells to increase VEGF secretion, potentially contributing to edema in airway remodeling.

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Comparative analysis of two types of CXCL8 from Japanese flounder (Paralichthys olivaceus)

Zhao

Katagiri

Kondo

et al. 2015

Developmental & Comparative Immunology

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Modulation of IL-8 mRNA expression and production in respiratory syncytial virus (RSV) stimulated human alveolar epithelial cells by budesonide and montelukast*1

Cited by 4 publications

References 0 publications

Molecular Characterization and Chemotactic Function of CXCL8 in Northeast Chinese Lamprey (Lethenteron morii)

Molecular Characterization and Chemotactic Function of CXCL8 in Northeast Chinese Lamprey (Lethenteron morii)

Vascular endothelial growth factor is increased by human pulmonary cells stimulated with Dermatophagoides sp. extract

Comparative analysis of two types of CXCL8 from Japanese flounder (Paralichthys olivaceus)

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