Modulation of Horizontally Acquired Genes by the Hha-YdgT Proteins in
            <i>Salmonella enterica</i>
            Serovar Typhimurium

Vivero, Aitziber; Baños, Rosa C.; Mariscotti, Javier F.; Oliveros, Juan Carlos; Portillo, Francisco García‐del; Juárez, Antonio; Madrid, Cristina

doi:10.1128/jb.01206-07

Cited by 60 publications

(71 citation statements)

References 39 publications

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“…When activated within macrophages, the SsrB protein promotes transcription of SPI-2, possibly by counteracting the H-NS-and YdgT-mediated repression (7). Indeed, deletion of the ydgT gene attenuated Salmonella virulence in a mouse infection model, which may have resulted from the expression of SPI-2 genes with inappropriate timing (i.e., under repressing conditions) and/or at inappropriate levels under inducing conditions (12,34).…”

Section: Discussionmentioning

confidence: 99%

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Choi

Shin

Yoon

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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SsrA/SsrB is a primary two-component system that mediates the survival and replication of Salmonella within host cells. When activated, the SsrB response regulator directly promotes the transcription of multiple genes within Salmonella pathogenicity island 2 (SPI-2). As expression of the SsrB protein is promoted by several transcription factors, including SsrB itself, the expression of SPI-2 genes can increase to undesirable levels under activating conditions. Here, we report that Salmonella can avoid the hyperactivation of SPI-2 genes by using ptsN-encoded EIIA Ntr , a component of the nitrogen-metabolic phosphotransferase system. Under SPI-2-inducing conditions, the levels of SsrB-regulated gene transcription increased abnormally in a ptsN deletion mutant, whereas they decreased in a strain overexpressing EIIA Ntr . We found that EIIA Ntr controls SPI-2 genes by acting on the SsrB protein at the posttranscriptional level. EIIA Ntr interacted directly with SsrB, which prevented the SsrB protein from binding to its target promoter. Finally, the Salmonella strain, either lacking the ptsN gene or overexpressing EIIA Ntr , was unable to replicate within macrophages, and the ptsN deletion mutant was attenuated for virulence in mice. These results indicated that normal SPI-2 gene expression maintained by an EIIA Ntr -SsrB interaction is another determinant of Salmonella virulence.virulence gene regulation | nitrogen-metabolic phosphotransferase system (PTS) | protein-protein interaction

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Section: Discussionmentioning

confidence: 99%

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Choi

Shin

Yoon

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…In addition, a transcriptomic study has shown that Hha and its paralogue YdgH could be involved in the regulation of the rck operon (Vivero et al, 2008). Fig.…”

Section: Rck Regulationmentioning

confidence: 99%

The Different Strategies Used by Salmonella to Invade Host Cells

Rosselin¹,

Abed²,

Namdari³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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“…At many loci, H-NS alone is insufficient for full transcriptional silencing, and members of the Hha/YdgT family of coregulatory proteins are also required (20,21). Hha and YdgT are small (8-kDa) protein paralogues that enhance repression at a subset of the H-NS regulon.…”

Section: The Bacterial Nucleoid-associated Proteins Hha and H-ns Joinmentioning

confidence: 99%

Structural Insights into the Regulation of Foreign Genes in Salmonella by the Hha/H-NS Complex

Ali

Whitney

Stevenson

et al. 2013

Journal of Biological Chemistry

108

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Modulation of Horizontally Acquired Genes by the Hha-YdgT Proteins in Salmonella enterica Serovar Typhimurium

Cited by 60 publications

References 39 publications

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

The Different Strategies Used by Salmonella to Invade Host Cells

Structural Insights into the Regulation of Foreign Genes in Salmonella by the Hha/H-NS Complex

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Modulation of Horizontally Acquired Genes by the Hha-YdgT Proteins in Salmonella enterica Serovar Typhimurium

Cited by 60 publications

References 39 publications

Salmonella pathogenicity island 2 expression negatively controlled by EIIA Ntr –SsrB interaction is required for Salmonella virulence

Salmonella pathogenicity island 2 expression negatively controlled by EIIA Ntr –SsrB interaction is required for Salmonella virulence

The Different Strategies Used by Salmonella to Invade Host Cells

Structural Insights into the Regulation of Foreign Genes in Salmonella by the Hha/H-NS Complex

Contact Info

Product

Resources

About

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence