The Different Strategies Used by Salmonella to Invade Host Cells

Rosselin, Manon; Abed, Nadia; Namdari, Fatémeh; Virlogeux-Payant, Isabelle; Velge, Philippe; Wiedemann, Agnès

doi:10.5772/29979

Cited by 4 publications

(4 citation statements)

References 122 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The infection process caused by P. salmonis uses clathrin for internalization and then the actin cytoskeleton for vacuole generation (Ramírez et al 2015). Similar pathways have been observed in other mammalian intracellular gram-negative bacteria (Manon et al 2012; Valencia-Gallardo et al 2015). Within the region associated with TD on Omy03 we identified a gene coding for the receptor DC-SIGN related with the immune response and expressed on macrophage and dendritic-cell surfaces (Ahmed et al 2015).…”

Section: Discussionsupporting

confidence: 81%

Single-Step Genome-Wide Association Study for Resistance to Piscirickettsia salmonis in Rainbow Trout (Oncorhynchus mykiss)

Barría

Marín-Nahuelpi

Cáceres

et al. 2019

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

One of the main pathogens affecting rainbow trout (Oncorhynchus mykiss) farming is the facultative intracellular bacteria Piscirickettsia salmonis. Current treatments, such as antibiotics and vaccines, have not had the expected effectiveness in field conditions. Genetic improvement by means of selection for resistance is proposed as a viable alternative for control. Genomic information can be used to identify the genomic regions associated with resistance and enhance the genetic evaluation methods to speed up the genetic improvement for the trait. The objectives of this study were to i) identify the genomic regions associated with resistance to P. salmonis; and ii) identify candidate genes associated with the trait in rainbow trout. We experimentally challenged 2,130 rainbow trout with P. salmonis and genotyped them with a 57 K single nucleotide polymorphism (SNP) array. Resistance to P. salmonis was defined as time to death (TD) and as binary survival (BS). Significant heritabilities were estimated for TD and BS (0.48 ± 0.04 and 0.34 ± 0.04, respectively). A total of 2,047 fish and 26,068 SNPs passed quality control for samples and genotypes. Using a single-step genome wide association analysis (ssGWAS) we identified four genomic regions explaining over 1% of the genetic variance for TD and three for BS. Interestingly, the same genomic region located on Omy27 was found to explain the highest proportion of genetic variance for both traits (2.4 and 1.5% for TD and BS, respectively). The identified SNP in this region is located within an exon of a gene related with actin cytoskeletal organization, a protein exploited by P. salmonis during infection. Other important candidate genes identified are related with innate immune response and oxidative stress. The moderate heritability values estimated in the present study show it is possible to improve resistance to P. salmonis through artificial selection in the rainbow trout population studied here. Furthermore, our results suggest a polygenic genetic architecture for the trait and provide novel insights into the candidate genes underpinning resistance to P. salmonis in O. mykiss.

show abstract

Section: Discussionsupporting

confidence: 81%

Single-Step Genome-Wide Association Study for Resistance to Piscirickettsia salmonis in Rainbow Trout (Oncorhynchus mykiss)

Barría

Marín-Nahuelpi

Cáceres

et al. 2019

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

show abstract

“…The infection process carried out by P. salmonis uses clathrin for internalization and then the actin cytoskeleton for vacuole generation (Ramírez et al 2015). Similar pathways have been observed in other mammalian intracellular gram-negative bacteria (Manon et al 2012; Valencia-gallardo et al 2015). Within the region associated with TD on Omy3 we identified a gene coding for the receptor DC-SIGN related with the immune response and expressed on macrophage and dendritic-cell surfaces (Ahmed et al 2015).…”

Section: Discussionsupporting

confidence: 81%

Single-step genome-wide association study for resistance toPiscirickettsia salmonisin rainbow trout (Oncorhynchus mykiss)

Marín-Nahuelpi

Barría

Cáceres

et al. 2019

Preprint

View full text Add to dashboard Cite

44One of the main pathogens affecting rainbow trout (Oncorhynchus mykiss) farming is the 45 facultative intracellular bacteria Piscirickettsia salmonis. Current treatments, such as 46 antibiotics and vaccines, have not had the expected effectiveness in field conditions. Genetic 47 improvement by means of selection for resistance is proposed as a viable alternative for 48 control. Genomic information can be used to identify the genomic regions associated with 49 resistance and enhance the genetic evaluation methods to speed up the genetic improvement 50 for the trait. The objectives of this study were to i) identify the genomic regions associated 51 with resistance to P. salmonis; and ii) identify candidate genes associated with the trait. We 52 experimentally challenged 2,130 rainbow trout with P. salmonis and genotyped them with a 53 57 K SNP array. Resistance to P. salmonis was defined as time to death (TD) and as binary 54 survival (BS). Significant heritabilities were estimated for TD and BS (0.48 ± 0.04 and 0.34 55 ± 0.04, respectively). A total of 2,047 fish and 26,068 SNPs passed quality control for 56 samples and genotypes. Using a single-step genome wide association analysis (ssGWAS) we 57 identified four genomic regions explaining over 1% of the genetic variance for TD and three 58 for BS. Interestingly, the same genomic region located on Omy27 was found to explain the 59 highest proportion of genetic variance for both traits (2.4 and 1.5% for TD and BS, 60 respectively). The identified SNP in this region is located within an exon of a gene related 61 with actin cytoskeletal organization, a protein exploited by P. salmonis during infection. 62Other important candidate genes identified are related with innate immune response and 63 oxidative stress. The moderate heritability values estimated in the present study show it is 64 possible to improve resistance to P. salmonis through artificial selection in the current 65 rainbow trout population. Furthermore, our results suggest a polygenic genetic architecture 66 4 and provide novel insights into the candidate genes underpinning resistance to P. salmonis 67 in O. mykiss. 68 69 70 71 72 As in any intensive animal production system, infectious diseases are one of the main 73 threats affecting the success and sustainability of aquaculture (Yáñez et al. 2014a). In the 74 case of salmonid production, one of the major pathogens affecting productivity is the 75 facultative intracellular bacteria Pisciricketssia salmonis, etiological agent of salmonid 76 rickettsial syndrome (SRS). This bacterium was first identified in 1989 in Chile, in a farmed 77 coho salmon (Oncorhynchus kisutch) population (Cvitanich et al. 1991). Since then, 78 mortalities resulting from SRS have been also identified in Atlantic salmon (Salmo salar) 79 and rainbow trout (Oncorhynchus mykiss) in several countries, such as Scotland, Ireland, 80 Norway and Chile (Fryer and Hedrick 2003). In Chile, SRS was responsible for 20.7, 67.9 81 and 92.6% of the mortalities associated with infectious disea...

show abstract

“…Moreover, downregulation of a set of invasion genes invAEFGH and upregulation of prgHIJK were observed after exposure of Enteritidis to KCOH at 4 mg/ml. Similar gene expression patterns were observed when Salmonella was exposed to bile or cationic peptides in the proximal small intestine or macrophage of host cell, respectively [38]. Reduced expression of genes involved in transcription regulators ( hilA , hilC ) were also reported with blueberry and blackberry pomace extracts against S .…”

Section: Discussionsupporting

confidence: 59%

“…Type-III secretion systems fulfil two significantly different roles in bacteria; first they act as an assembly and export system in the production of the flagellum (Flagellar- or F-T3SSs) and second, they translocate effector proteins into host cells [37]. Translocation of effector proteins requires an exportation apparatus controlled by spaP , spaQ , spaR , spaS , invA , invC and orgB [38]. More than 25 genes are required for the host-invasion by S .…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of Salmonella enterica serovar Enteritidis exposed to ethanolic extract of organic cranberry pomace

Das

Lepp

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

Non-typhoidal Salmonella enterica serovars continue to be an important food safety issue worldwide. Cranberry ( Vaccinium macrocarpon Ait ) fruits possess antimicrobial properties due to their various acids and phenolic compounds; however, the underlying mechanism of actions is poorly understood. We evaluated the effects of cranberry extracts on the growth rate of Salmonella enterica serovars Typhimurium, Enteritidis and Heidelberg and on the transcriptomic profile of Salmonella Enteritidis to gain insight into phenotypic and transcriptional changes induced by cranberry extracts on this pathogen. An ethanolic extract from cranberry pomaces (KCOH) and two of its sub-fractions, anthocyanins (CRFa20) and non-anthocyanin polyphenols (CRFp85), were used. The minimum inhibitory (MICs) and bactericidal (MBCs) concentrations of these fractions against tested pathogens were obtained using the broth micro-dilution method according to the Clinical Laboratory Standard Institute’s guidelines. Transcriptional profiles of S . Enteritidis grown in cation-adjusted Mueller-Hinton broth supplemented with or without 2 or 4 mg/ml of KCOH were compared by RNASeq to reveal gene modulations serving as markers for biological activity. The MIC and MBC values of KCOH were 8 and 16 mg/mL, respectively, against all tested S . enterica isolates. The MIC value was 4 mg/mL for both CRFa20 and CRFp85 sub-fractions, and a reduced MBC value was obtained for CRFp85 (4 mg/ml). Treatment of S . Enteritidis with KCOH revealed a concentration-dependent transcriptional signature. Compared to the control, 2 mg/ml of KCOH exposure resulted in 89 differentially expressed genes (DEGs), of which 53 and 36 were downregulated and upregulated, respectively. The upregulated genes included those involved in citrate metabolism, enterobactin synthesis and transport, and virulence. Exposure to 4 mg/ml KCOH led to the modulated expression of 376 genes, of which 233 were downregulated and 143 upregulated, which is 4.2 times more DEGs than from exposure to 2 mg/ml KCOH. The downregulated genes were related to flagellar motility, Salmonella Pathogenicity Island-1 (SPI-1), cell wall/membrane biogenesis, and transcription. Moreover, genes involved in energy production and conversion, carbohydrate transport and metabolism, and coenzyme transport and metabolism were upregulated during exposure to 4 mg/ml KCOH. Overall, 57 genes were differentially expressed (48 downregulated and 9 upregulated) in response to both concentrations. Both concentrations of KCOH downregulated expression of hilA , which is a major SPI-1 transcriptional regulator. This study provides information on the response of Salmonella exposed to cranberry extracts, which could be used in the control of this important foodborne pathogen.

show abstract

The Different Strategies Used by Salmonella to Invade Host Cells

Cited by 4 publications

References 122 publications

Single-Step Genome-Wide Association Study for Resistance to Piscirickettsia salmonis in Rainbow Trout (Oncorhynchus mykiss)

Single-Step Genome-Wide Association Study for Resistance to Piscirickettsia salmonis in Rainbow Trout (Oncorhynchus mykiss)

Single-step genome-wide association study for resistance toPiscirickettsia salmonisin rainbow trout (Oncorhynchus mykiss)

Transcriptional profiling of Salmonella enterica serovar Enteritidis exposed to ethanolic extract of organic cranberry pomace

Contact Info

Product

Resources

About