Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (<i>Cucumis sativus</i>L.)

Cantarello, Corrado; Volpe, Veronica; Azzolin, Chiara M.M.; Bertea, Cinzia M.

doi:10.1080/17429140600831581

Cited by 37 publications

(20 citation statements)

References 71 publications

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“…thaliana wherein several phenylpropanoid metabolism genes were downregulated in plants exposed to UV radiation 67 . However, the sensitivity of plants to UV radiation has been shown to vary with different plant species 68 . The antioxidant and senescence marker genes SOD and SAG were upregulated, possibly indicating elevated demand for ROS scavenging mechanisms due to UV stress.…”

Section: Resultsmentioning

confidence: 99%

Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus

Nishanth

Sheshadri

Rathore

et al. 2018

Sci Rep

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Catharanthus roseus is a commercial source for anti-cancer terpenoid indole alkaloids (TIAs: vincristine and vinblastine). Inherent levels of these TIAs are very low, hence research studies need to focus on enhancing their levels in planta. Since primary metabolism provides precursors for specialized-metabolism, elevating the former can achieve higher amounts of the latter. Cell Wall Invertase (CWIN), a key enzyme in sucrose-metabolism catalyses the breakdown of sucrose into glucose and fructose, which serve as carbon-skeleton for specialized-metabolites. Understanding CWIN regulation could unravel metabolic-engineering approaches towards enhancing the levels of TIAs in planta. Our study is the first to characterize CWIN at gene-expression level in the medicinal plant, C. roseus. The CWINs and their inter-relationship with sucrose and TIA metabolism was studied at gene and metabolite levels. It was found that sucrose-supplementation to C. roseus leaves significantly elevated the monomeric TIAs (vindoline, catharanthine) and their corresponding genes. This was further confirmed in cross-species, wherein Nicotiana benthamiana leaves transiently-overexpressing CrCWIN2 showed significant upregulation of specialized-metabolism genes: NbPAL2, Nb4CL, NbCHS, NbF3H, NbANS, NbHCT and NbG10H. The specialized metabolites- cinnamic acid, coumarin, and fisetin were significantly upregulated. Thus, the present study provides a valuable insight into metabolic-engineering approaches towards augmenting the levels of therapeutic TIAs.

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Section: Resultsmentioning

confidence: 99%

Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus

Nishanth

Sheshadri

Rathore

et al. 2018

Sci Rep

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“…The role of PAL as a key enzyme activating phenylpropanoid biosynthesis in response to UV-B radiation has been studied for many years. For example, increased PAL expression after UV-B exposure has been reported in mesophyll cells of Pisum sativum (Kalbin et al 2001), leaves of Petunia (Ryan et al 2002), Cucumis sativus (Cantarrello et al 2005) and in rosette leaves of A. thaliana (Ulm et al 2004). It has also been reported that UV-B exclusion results in lower PAL activity in L. sativa (Krizek et al 1998).…”

Section: Discussionmentioning

confidence: 96%

Effects of solar UV-A and UV-B radiation on gene expression and phenolic accumulation in Betula pendula leaves

Morales¹,

Tegelberg²,

Brosché³

et al. 2010

Tree Physiology

143

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Ultraviolet (UV) radiation is an important environmental factor for plant communities; however, plant responses to solar UV are not fully understood. Here, we report differential effects of solar UV-A and UV-B radiation on the expression of flavonoid pathway genes and phenolic accumulation in leaves of Betula pendula Roth (silver birch) seedlings grown outdoors. Plants were exposed for 30 days to six UV treatments created using three types of plastic film. Epidermal flavonoids measured in vivo decreased when UV-B was excluded. In addition, the concentrations of six flavonoids determined by high-performance liquid chromatography-mass spectrometry declined linearly with UV-B exclusion, and transcripts of PAL and HYH measured by quantitative real-time polymerase chain reaction were expressed at lower levels. UV-A linearly regulated the accumulation of quercetin-3-galactoside and quercetin-3-arabinopyranoside and had a quadratic effect on HYH expression. Furthermore, there were strong positive correlations between PAL expression and accumulation of four flavonols under the UV treatments. Our findings in silver birch contribute to a more detailed understanding of plant responses to solar UV radiation at both molecular and metabolite levels.

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“…We hypothesize that the accumulation of polyphenols observed after storage could be the result of metabolic processes induced by UV-B exposure and started before leaf sampling. Indeed, UV-B treatment seems to increase not only the transcription of PAL (the gene of phenylalanine ammonia-lyase [ 19 , 37 ]), but also directly the PAL activity [ 37 , 38 , 39 , 40 ]. The carbon used for polyphenol biosynthesis could derive by the release of soluble carbohydrates caused by changes in the activity of sucrose-hydrolyzing and sucrose-synthesizing enzymes as well as by higher respiratory rates, since, as has been reported in the literature, both these processes are positively affected by UV-B treatments [ 39 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Short-Term Pre-Harvest UV-B Supplement Enhances the Polyphenol Content and Antioxidant Capacity of Ocimum basilicum Leaves during Storage

Nascimento

Brunetti

Agati

et al. 2020

Plants

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Ocimum basilicum (basil) leaves are rich in polyphenols, conferring them a high antioxidant activity. The application of UV-B can be used to maintain the post-harvest nutraceutical quality of basil leaves. We aimed to investigate the effects of pre-harvest UV-B application on polyphenolic and pigment contents, antioxidant capacity, and the visual quality of basil stored leaves. We also evaluated the applicability of the non-invasive Dualex® for monitoring the accumulation of leaf epidermal phenolics (Flav Index). After exposing plants to white light (control) and to supplemental UV-B radiation for 4 d, the leaves were harvested and stored for 7d (TS7). The UV-B leaves showed both a higher phenolic content and antioxidant capacity than the controls at TS7. In addition, the correlations between the Flav Index and phenolic content demonstrated that Dualex® can reliably assess the content of epidermal phenolics, thus confirming its promising utilization as a non-destructive method for monitoring the phytochemical quality of O. basilicum leaves. In conclusion, a pre-harvesting UV-B application may be a tool for enhancing the content of polyphenols and the antioxidant potential of basil stored leaves without detrimental effects on their visual quality. These results are important considering the nutraceutical value of this plant and its wide commercial distribution.

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Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (Cucumis sativusL.)

Cited by 37 publications

References 71 publications

Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus

Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus

Effects of solar UV-A and UV-B radiation on gene expression and phenolic accumulation in Betula pendula leaves

Short-Term Pre-Harvest UV-B Supplement Enhances the Polyphenol Content and Antioxidant Capacity of Ocimum basilicum Leaves during Storage

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