Modulation of collagen production in cultured fibroblasts by a low-frequency, pulsed magnetic field

Murray, J.; Farndale, Richard W.

doi:10.1016/0304-4165(85)90255-7

Cited by 95 publications

(40 citation statements)

References 25 publications

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“…Collagen synthesis and deposition are important for wound healing and development of wound strength (Falanga 2005). Various studies have shown that collagen production could be promoted by some mechanical stimuli, such as ultrasound (Olerud et al 1990), pulsating electromagnetic field (Murray and Farndale 1985) and mechanical pressure or stretch force (Chan et al 2010). In this study, we observed that the enhanced wound breaking strength correlated well with the increased hydroxyproline content in both ESWT groups.…”

Section: Discussionsupporting

confidence: 53%

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

Yang

Luo

Yan

et al. 2011

Tohoku J. Exp. Med.

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Impaired wound healing in surgical patients with diabetes increases the incidence of infection, prolongs hospitalization, and even increases the rate of mortality. Low-energy extracorporeal shock wave treatment (ESWT) was reported to accelerate chronic wound healing by promoting revascularization and tissue regeneration; however, it is not known if ESWT could also improve healing of acute surgical incisional wounds in diabetes. In this study, using a rat model of diabetes, we investigated the effect of low-energy ESWT on collagen content in wound tissues and its efficacy in incisional wound healing. A single dorsal incisional wound was inflicted in streptozotocin-induced diabetic rats, and they received ESWT at different time post-wounding. Rats were sacrificed on days 7 and 14 post-wounding. Wound breaking strength, hydroxyproline content, histological characteristics and the expression of transforming growth factor beta 1 (TGF-β1) were analyzed. As a result, the wound breaking strength was significantly enhanced and the hydroxyproline content in wound tissues was increased at each time point examined. The number of fibroblasts was significantly increased, and the new collagen fibers were more abundant at the wound site after ESWT. Furthermore, the expression of TGF-β1 was up-regulated after ESWT on day 7 post-wounding. These results suggest that low-energy ESWT can increase collagen content, enhance wound breaking strength and improve the healing of incisional wound in diabetic rats. The increased collagen content may be attributed, at least in part, to the up-regulation of TGF-β1 expression in wound tissues.

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Section: Discussionsupporting

confidence: 53%

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

Yang

Luo

Yan

et al. 2011

Tohoku J. Exp. Med.

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“…PEMF appeared to have no effect on the interaction between the cytokine and synovial fibroblasts, since it did not alter the response of the fibroblasts to that signal in terms of prostaglandin or collagenase production. These data also confirm our previous observation (29) that PEMF has no effect on either production or latency of collagenase in cultured tendon fibroblasts. Cruess et al (9) onstrated in a rat model of disuse osteoporosis that the application in vivo of PEMF similar to those reported here caused a significant reduction in the high levels of collagenase associated with the osteoporotic bone fragments cultured in vitro; after PEMF treatment, collagenase levels fell to those of normal bone.…”

Section: Discussionsupporting

confidence: 94%

“…These apparently contradictory findings may reflect differences between whole tissue responses (and their inherent complexity) and those of isolated cells. We have recently demonstrated (29) that chronic exposure of isolated chick tendon fibroblasts to PEMF reduces cAMP levels and, we believe as a consequence, diminishes the intracellular breakdown of newly formed collagen. This pathway is also thought to involve the phagolysosome system.…”

Section: Murray Et Almentioning

confidence: 97%

Degradative pathways in cultured synovial fibroblasts: Selective effects of pulsed electromagnetic fields

Murray¹,

Lacy²,

Jackson³

1988

Journal Orthopaedic Research

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A cell culture model for studying the cytokine-mediated degradation of connective tissue was exposed to clinically applied, low-frequency pulsed electromagnetic fields (PEMF), and levels of collagenolytic activity, two lysosomal hydrolases, and prostaglandin E2 were measured. PEMFs reduced the release of two lysosomal enzymes by cultured rabbit synovial fibroblasts but did not affect their response to mononuclear-cell-conditioned medium. PEMF did not alter levels of cytokine activity produced by a mixed mononuclear cell population, nor did they affect the cytokine-mediated release of collagenase or prostaglandin E2 by synovial fibroblasts. The relevance of these findings to the clinical application of PEMF to soft- and hard-tissue injuries is discussed.

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“…When the same pulsed electromagnetic field employed in this study was used, collagen synthesis was found to be stimulated approximately 70% in fibroblasts as measured by 3H-proliiie incorporation Murray and Farndale, 1985). Thus far endothelial cell extracellular matrix synthesis has not been monitored in the presence of pulsed electromagnetic fields.…”

Section: Discussionmentioning

confidence: 98%

Endothelial cell response to pulsed electromagnetic fields: Stimulation of growth rate and angiogenesis in vitro

Yen-Patton

Patton

Beer

et al. 1988

Journal Cellular Physiology

153

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The effects of pulsed electromagnetic fields on the repopulation rate of denuded regions of endothelial cell monolayers and on endothelial cell reorganization into complex vessellike structures was monitored in vitro by using human umbilical vein and bovine aortic endothelial cells. A small (20-40%) but statistically significant enhancement in growth rate of partially denuded endothelial cell monolayers as determined by tritiated thymidine incorporation was observed in the presence of pulsed electromagnetic fields. Morphologically, endothelial cells entering the denuded regions were observed to be elongated, often connecting end to end to form a mycelial or "sprouting" pattern when exposed to pulsed electromagnetic fields. This was in contrast to cells outside of the field which had a more cuboidal morphology. Complete disruption of the endothelial cell monolayer by passaging the cells with EDTA-trypsin resulted in reorganization of some of the cells into three-dimensional vessellike structures after as little as 5-8 hours in the presence of the pulsed electromagnetic field. This reorganization occurred in the presence of heparin, endothelial cell growth factor, and a competent fibronectin matrix. Vascularization for comparable cultures outside of the field did not occur during the time-course of the experiments. Discrete stages of neovascularization were observed in the presence of the field that were qualitatively similar to stages of angiogenesis observed in vivo.

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Modulation of collagen production in cultured fibroblasts by a low-frequency, pulsed magnetic field

Cited by 95 publications

References 25 publications

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

Degradative pathways in cultured synovial fibroblasts: Selective effects of pulsed electromagnetic fields

Endothelial cell response to pulsed electromagnetic fields: Stimulation of growth rate and angiogenesis in vitro

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