Modulation of cell adhesion by modification of titanium surfaces with covalently attached self‐assembled monolayers

Sukenik, Chaim N.; Balachander, Natarajan; Culp, Lloyd A.; Lewandowska, Kristine; Merritt, K.

doi:10.1002/jbm.820241004

Cited by 87 publications

(38 citation statements)

References 41 publications

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“…Since then many studies in neuronal patterning have been performed in a variety of labs, using various methods, including surface modification by silane chemistry 113 137 Ti, 140 polystyrene, 130,131 Teflon (fluorinated ethylene propylene), [132][133][134][141][142][143][144] and biodegradable polymers. 145 These studies mainly yielded insights into the adhesion and guidance of neuronal growth by insoluble factors and could prove valuable for selective neuronal immobilization on future microsensors.…”

Section: Vb Neuronal Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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Section: Vb Neuronal Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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“…[31][32][33] Silane-modified surfaces have been widely utilized as model surfaces for biological interactions, including protein adsorption [34][35][36] and cellular and bacterial adhesion. [37][38][39] However, previous reports of cellular responses to silanated surfaces generally have been restricted to short-term studies of endothelial cells or fibroblast cell lines and have not yet addressed many of the significant cell-biomaterial interactions that occur throughout the duration of the inflammatory stage. Therefore, our investigation focuses on the dynamic interactions between silane-modified glass and freshly isolated human peripheral blood monocytes as an in vitro model for the monocyte-macrophage- Figure 1.…”

Section: Introductionmentioning

confidence: 98%

Human monocyte/macrophage adhesion, macrophage motility, and IL-4-induced foreign body giant cell formation on silane-modified surfacesin vitro

Jenney

DeFife

Colton

et al. 1998

J. Biomed. Mater. Res.

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A cytokine-based, in vitro model of foreign body giant cell (FBGC) formation was utilized to examine the effect of biomaterial surface chemistry on the adhesion, motility, and fusion of monocytes and macrophages. Human monocytes were cultured for 10 days on 14 different silane-modified glass surfaces, during which time the cells assumed the macrophage phenotype. The adhesion of monocytes and macrophages during the culture period decreased by an average of approximately 50%, with the majority of cell loss observed during days 1-3. Most important, the adhesion of monocytes and macrophages was surface independent except for two surfaces containing terminal methyl groups, which decreased adhesion levels. Interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) were added to the medium to induce FBGC formation and enhance macrophage adhesion, respectively. Surprisingly, GM-CSF decreased long-term monocyte/macrophage adhesion. IL-4-induced FBGC density was strongly influenced by the surface carbon content, as determined by X-ray photoelectron spectroscopy (XPS). In contrast, contact angle and surface energy displayed no correlation with FBGC formation. The motility of adherent macrophages, as measured by time-lapse confocal microscopy, was not affected significantly by differences in surface chemistry or the addition of cytokines. The surface dependence of FBGC formation is hypothesized to be the result of varying levels of silane-derived surface carbon.

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“…The density and mobility of recognition centres are therefore important design parameters during development of biosensing interfaces. The impact of surface chemistry on protein adsorption also has been demonstrated on several occasions using various types of SAMs, not just on gold but on Si, Ti and glass as well [7,[10][11][12][13]. Prime et al, for example, investigated the protein resistance of flexible long-chain oligo(ethylene) oxide modified SAMs [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…In the case of longer versus shorter alkyl chains it is believed that thermodynamics play a major role for the phase behavior [16,17]. Bain et al studied a series of mixed SAMs from HS -(CH 2 ) 11 -CH 3 /HS -(CH 2 ) n -CH 3 (n= 15,21) adsorbed from ethanol and isooctane [18]. Using contact angle measurements, ellipsometry, and XPS they concluded that macroscopic islands do not form in these monolayers.…”

Section: Introductionmentioning

confidence: 99%

Order/disorder gradients of n-alkanethiols on gold

Lestelius

Engquist

Tengvall

et al. 1999

Colloids and Surfaces B: Biointerfaces

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This paper explores the interfacial properties of one-dimensional molecular gradients of alkanethiols (HS -(CH 2 ) n -X) on gold. The kinetics and thermodynamics of monolayer formation are important issues for these types of mixed molecular assemblies. The influence of chain length difference on the contact angles with hexadecane (HD), q a and q r , and the hysteresis, has been studied by employing alkanethiols HS -(CH 2 ) n -CH 3 , with n=9, 11, 13, 15 and 17, in the preparation of the self-assembled monolayers (SAM) gradients. The contact angles with hexadecane, at the very extreme ends of the gradients, show characteristic values of a highly ordered CH 3 -like assembly: q a = 45 -50°. In the middle of the gradients q a drops noticeably and exhibits values representative for CH 2 -like polymethylenes, q a =20-30°, indicating a substantial disordering of the protruding chains of the longer component in the gradient assembly. As expected, the exposure of CH 2 -groups to the probing liquid increases with increasing differential chain length of the two n-alkanethiol used, in this case eight methylene units. However, the contact angles always display a non-zero value which means that even at a chain length difference of eight methylene units there is a substantial exposure of methyl (CH 3 ) groups to the probing liquid. With infrared reflection-absorption spectroscopy (IRAS) we have monitored the structural behavior of the polymethylene chains along the gradient. We find complementary evidence for disordered chains in the gradient region, and the IRAS results correlate well with the contact angle measurements.

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Modulation of cell adhesion by modification of titanium surfaces with covalently attached self‐assembled monolayers

Cited by 87 publications

References 41 publications

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Human monocyte/macrophage adhesion, macrophage motility, and IL-4-induced foreign body giant cell formation on silane-modified surfacesin vitro

Order/disorder gradients of n-alkanethiols on gold

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