Modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from Bacteroides gingivalis

Millar, Stephen J.; Goldstein, Elaine G.; Levine, Martin; Hausmann, Ernest

doi:10.1128/iai.51.1.302-306.1986

Cited by 81 publications

(59 citation statements)

References 21 publications

(16 reference statements)

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“…In periodontitis, periodontal tissues are continuously challenged by periodontopathogens, such as P. gingivalis. It was shown that P. gingivalis could mediate the destruction of periodontal tissue and could be involved in alveolar bone resorption in various ways (40)(41)(42)(43)(44). In addition, the products of P. gingivalis were reported to have the ability to induce and activate host MMPs (19)(20)(21)(22)(23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of green tea polyphenol (–)‐epigallocatechin gallate on the expression of matrix metalloproteinase‐9 and on the formation of osteoclasts

Yun

Pang

Kim

et al. 2004

J of Periodontal Research

140

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Section: Discussionmentioning

confidence: 99%

Inhibitory effects of green tea polyphenol (–)‐epigallocatechin gallate on the expression of matrix metalloproteinase‐9 and on the formation of osteoclasts

Yun

Pang

Kim

et al. 2004

J of Periodontal Research

140

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“…gingivalis did not decrease the ALP activity in MC3T3-E 1 cells. Accordingly, the tion in vitro mediated by prostaglandin E 2 and interleukin-1␤ (Nair et al, 1983;Millar et al, 1986; Bom-van Noorloos et extracts of Porph. gingivalis are not the inhibitory agent of bone formation.…”

Section: Discussionmentioning

confidence: 99%

Extracts of Prevotella intermedia and Actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro

et al. 1997

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In order to study the effects of sonicated extracts from Prevotella intermedia, Actinobacillus actinomycetemcomit‐ans, Porphyromonas gingivalis, and other oral‐related bacteria, as well as Escherichia coli on bone formation, clone MC3T3‐E1 cells, which have retained osteoblastic activity, were cultured with various bacterial extracts. The addition of the sonicated extracts from Prevotella intermedia and Actinobacillus actinomycetemcomitans decreased the alkaline phosphatase activity in a dose‐dependent fashion over the concentration range of 1– 1000 ng ml‐1 compared with the control. By contrast, the sonicated extracts from other oral bacteria including Porphyromonas gingivalis, Capnocytophaga ochracea, Streptococcus milleri and Streptococcus sanguis, and Escherichia coli did not decrease the alkaline phosphatase activity even in the presence of 100 ng ml‐1 protein. The addition of Prevotella intermedia and Actinobacillus actinomycetemcomitans extracts that had been treated with heat and trypsin to the cell cultures also inhibited alkaline phosphatase activity in the cells, suggesting that inhibitory factors are not proteinaceouS. Polymyxin B did not change the alkaline phosphatase activity in the cells treated with the extracts from Prevotella intermedia and Actinobacillus actinomycetemcomitans, suggesting that the inhibitory activity of the extracts is not lipopolysaccha‐ride. The inhibitory effect of both extracts was observed in the molecular mass over 290 kDa eluted from Sephadex G‐200 column. The inhibitory substances of Prevotella intermedia were partially purified and showed broad band with estimated molecular weight of 170–190 kDa by sodium dodecyl sulfate‐polyacrylamide gel electro‐phoresis. These results indicate that Prevotella intermedia and Actinobacillus actinomycetemcomitans may play an important role in inhibiting bone formation as well as in stimulating bone resorption.

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“…Humoral and cellular immune components are thought to pass through tissue interfaces which allows for relatively large molecular weight products to can gain entry to the systemic circulation and be made available to serve as nonspecific or antigenic stimulators of immune effector cells. Preliminary studies have shown that LPS derived from BG can induce bone resorption in vitro and may be important in this regard relative to periapical and periodontal bone loss (20). Interleukin 1 (IL-1) is a small polypeptide monokine known to induce collagen production, osseous remodeling.…”

mentioning

confidence: 99%

Cytotoxic and immunostimulatory effects of Bacteroides cell products

Fotos

Lewis

Gerencser

et al. 1990

J Oral Pathology Medicine

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The etiologic role of Bacteroides in both periodontal and periapical infections has been well documented, with current interest focusing on the specific pathogenic mechanisms involved. The effects of cell fractions derived from Bacteroides gingivalis (BG), Bacteroides intermedius (BI), and Bacteroides asaccharolyticus (BA) have been studied in vitro through: an assessment of the direct cytotoxic effects on human gingival fibroblasts using a tetrazolium dye reduction assay, an evaluation of murine lymphocyte stimulation and interleukin-1 release, and the induction of human lymphocyte-mediated cytotoxicity. Both BG and BI stimulated interleukin-1 release (P less than 0.001), while BA, a nonoral organism, was not significantly active in this respect. Only BG sonicates were able to induce lymphocyte-mediated cytotoxicity (P less than 0.005). All three Bacteroides species demonstrated direct cytotoxic effects on cultured gingival fibroblasts, and these effects were related to the relative protein content and endotoxin activity of the sonicate preparations for each organism. These data show that BG and BI possess factors which may enhance their virulence through activities not shared with BA.

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Modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from Bacteroides gingivalis

Cited by 81 publications

References 21 publications

Inhibitory effects of green tea polyphenol (–)‐epigallocatechin gallate on the expression of matrix metalloproteinase‐9 and on the formation of osteoclasts

Inhibitory effects of green tea polyphenol (–)‐epigallocatechin gallate on the expression of matrix metalloproteinase‐9 and on the formation of osteoclasts

Extracts of Prevotella intermedia and Actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro

Cytotoxic and immunostimulatory effects of Bacteroides cell products

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