Inhibitory effects of green tea polyphenol (–)‐epigallocatechin gallate on the expression of matrix metalloproteinase‐9 and on the formation of osteoclasts

Yun, Jin-Mun; Pang, Eun-Kyoung; Kim, Chang Sung; Yoo, Yun‐Jung; Cho, Kyoo-Sung; Chai, Jung‐Kiu; Kim, Chong Kwan; Choi, Seong‐Ho

doi:10.1111/j.1600-0765.2004.00743.x

Cited by 139 publications

(114 citation statements)

References 58 publications

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“…These data suggest that the preparation might signifi cantly attenuate tumor progression by blocking MMP-9 synthesis. The main polyphenol from green tea, EGCG, likewise the polyphenols tested, was able to decrease MMP-9 expression in 95-D lung carcinoma cells at the concentration of 40 μmol/L [Yang et al, 2005], whereas in periodontal diseases it was effective even at the concentration of only 20 μmol/L [Yun et al, 2004].…”

Section: Discussionmentioning

confidence: 99%

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Szewczyk¹,

Lewandowska²,

Owczarek³

et al. 2014

Pol. J. Food Nutr. Sci.

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Key words: evening primrose, matrix metalloproteinases, cancer Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid --linolenic acid (GLA) -but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the infl uence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography.The results revealed that the examined polyphenol extract had little infl uence on Caco-2 proliferation, but effectively in a time-and dose-dependent manner inhibited MMP-1, MMP-7, MMP-9 and MMP-14 mRNA synthesis induced by TNF- and TPA. Additionally, zymographic analysis revealed that after 24 h, the polyphenol extract at a concentration of 50 mol/L GAE caused a 10-fold reduction in MMP-9 synthesis. Moreover, this extract might be a potent inhibitor of MMP activity. The results showed that polyphenol extract from evening primrose inhibited PBMC-derived MMP-2 and MMP-9 enzymatic activity in dose-dependent manner. The obtained results indicate that the polyphenol extract from evening primrose seeds could be an inhibitor of proteases involved in tumor progression and metastasis.

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Section: Discussionmentioning

confidence: 99%

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Szewczyk¹,

Lewandowska²,

Owczarek³

et al. 2014

Pol. J. Food Nutr. Sci.

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“…The microbiological techniques used in this study are widely evident and referenced in the literature 22 . However, one important limitation of this type of study is the high sensitivity for detecting aerobic microorganisms, but low sensitivity for detecting anaerobic microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Antoniazzi

Trojahn

Casarin³

et al. 2016

Rev. odontol. UNESP

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ResumoObjetivo: Comparar a eficácia antimicrobiana de clorexidina 0,12% (CHX) e solução de chá verde (CV) (Camellia sinensis) em biofilmes formados em diferentes tensões de oxigênio em um modelo in situ. Método: Vinte e cinco estudantes de odontologia foram elegíveis. Um dispositivo com espécimes de esmalte (EE) padronizados, voltado para o palato e para vestibular foram inseridos nos voluntários por 7 dias. Durante os primeiros quatro dias nenhum agente foi aplicado. A partir do quinto dia, ambos os agentes foram aplicados nos EEs no grupo teste e nenhum agente foi aplicado no grupo EE controle. Após 7 dias, os fragmentos EEs foram removidos dos dispositivos, sonicados, plaqueados em ágar, e incubado durante 24 h a 37 °C para determinar unidades formadoras de colonias (UFCs). Resultado: CHX teve significativamente melhor eficácia em comparação com CV nos EEs para vestibular (1.330 vs. 2.170 UFC/mL) e EEs voltados para o palatal (2.250 vs. 2.520 UFC/mL). Comparações intragrupos mostraram maior eficácia em EEs vestibulares do que as EEs voltadas para o palato (1.330 vs. 2.250 UFC/mL para CHX e 2170 vs, 2520 UFC/mL para CV) em ambas as soluções. Análise dos controles, mostrou a formação de biofilme significativamente maior em comparação com EEs palatinas e bucais. Conclusão: CHX tem uma eficácia maior do que o chá verde em biofilmes formados por 4 dias. A eficácia dos agentes foi reduzida para os biofilmes em ambiente com baixa tensão de oxigênio. A tensão de oxigênio parece influenciar na eficácia dos agentes testados.Descritores: Camellia sinensis; clorexidina; placa dentária. AbstractObjective: To compare the antimicrobial efficacy of a 0.12% chlorhexidine (CHX) and herbal green tea (Camellia sinensis) solution on established biofilms formed at different oxygen tensions in an in situ model. Method: Twenty-five dental students were eligible for the study. In situ devices with standardized enamel specimens (ES) facing the palatal and buccal sides were inserted in the mouths of volunteers for a 7 day period. No agent was applied during the first four days. From the fifth day onward, both agents were applied to the test ES group and no agent was applied to the control ES group. After 7 days the ES fragments were removed from the devices, sonicated, plated on agar, and incubated for 24 h at 37 °C to determine and quantify the colony forming units (CFUs). Result: CHX had significantly higher efficacy compared to green tea on the buccal (1330 vs. 2170 CFU/µL) and palatal (2250 vs. 2520 CFU/µL) ES. In addition, intragroup comparisons showed significantly higher efficacy in buccal ES over palatal ES (1330 vs. 2250 CFU/µL for CHX and 2170 vs, 2520 CFU/µL for CV) for both solutions. Analysis of the ES controls showed significantly higher biofilm formation in palatal ES compared to buccal ES. Conclusion: CHX has higher efficacy than green tea on 4-day biofilms. The efficacy of both agents was reduced for biofilms grown in a low oxygen tension environment. Therefore, the oxygen tension environment seems to influence the eff...

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“…Seu pH fica entre 7,4 a 7,8. O armazenamento em água de torneira pode ser tão destrutivo para o ligamento periodontal, quanto deixar o dente seco, levando a anquilose e a reabsorção por substituição 26,28,29 .…”

Section: áGua De Torneiraunclassified

“…A saliva contém substâncias potencialmente prejudiciais, tais como enzimas, bactérias e seus subprodutos 26 . A saliva, teve uma performance insatisfatória, em termos de preservação do ligamento em dentes controlados radiograficamente.…”

Section: Salivaunclassified

Meios De Armazenamento Para Dentes Avulsionados - Uma Revisão De Literatura

Flôres¹,

Flores

Diesel

et al. 2016

Saúde (Sta. Maria)

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RESUMOO objetivo deste estudo foi encontrar, através de uma revisão de literatura, o meio mais adequado para o armazenamento de dentes avulsionados. Esse meio deve preservar a vitalidade, aderência e a capacidade clonogênica das células do ligamento periodontal. Os meios selecionados para essa revisão de literatura foram: solução salina balanceada de Hank (HBSS), própolis, água de coco, leite, chá verde, água de torneira, clara de ovo, saliva, Gatorade, amora, ViaSpan, chá verde, água de coco, extrato de amora, própolis e clara de ovo apresentaram bons resultados nos estudos selecionados, porém necessitam mais estudos que comprovem sua eficácia. HBSS e ViaSpan tiveram os melhores resultados, apresentando grande potencial de manter as células do ligamento periodontal viáveis após a avulsão. O leite demonstrou resultados satisfatórios, boas propriedades, baixo custo e fácil disponibilidade à população, sendo este o meio ideal para armazenamento de dentes avulsionados, quando HBSS e ViaSpan forem indisponíveis. Descritores ABSTRACTThe aim of this study was to find, through a literature review, the most appropriate means for storage of avulsed teeth. Such means shall preserve the vitality, adherence and clonogenic capacity of cells of the periodontal ligament. The selected means for this literature review were: Hank's balanced salt solution (HBSS), propolis, coconut water, milk, green tea, tap water, egg white, saliva, Gatorade, blackberry, ViaSpan. green tea, coconut water, mulberry extract, propolis and egg white showed good results in selected studies, but need more studies to prove its effectiveness. HBSS and ViaSpan had the best results, presenting great potential to maintain the cells in viable periodontal ligament after avulsion. The milk was satisfactory, good properties, low cost and easy availability to the population, which is the ideal medium for storage of avulsed teeth when HBSS and ViaSpan are unavailable.

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Inhibitory effects of green tea polyphenol (–)‐epigallocatechin gallate on the expression of matrix metalloproteinase‐9 and on the formation of osteoclasts

Abstract: These findings suggest that EGCG may prevent the alveolar bone resorption that occurs in periodontal diseases by inhibiting the expression of MMP-9 in osteoblasts and the formation of osteoclasts.

Cited by 139 publications

References 58 publications

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Meios De Armazenamento Para Dentes Avulsionados - Uma Revisão De Literatura

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