Modulation of body fat and serum leptin levels by dietary conjugated linoleic acid in Sprague-Dawley rats fed various fat-level diets

Yamasaki, Masao; Ikeda, Atsushi; Oji, Mariko; Tanaka, Yoko; Hirao, Akira S.; Kasai, Michio; Iwata, Toshio; Tachibana, Hirofumi; Yamada, Koji

doi:10.1016/s0899-9007(02)00842-0

Cited by 87 publications

(62 citation statements)

References 26 publications

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“…[7][8][9][10] The antiobesity effects of CLA have been supported by studies in animals. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] Most of these studies used synthetically prepared CLA, a mixture of different isomers. Commercially prepared CLA supplements usually contain two major isomers, c9t11 and trans-10, cis-12 (t10c12), in equal amounts.…”

Section: Introductionmentioning

confidence: 99%

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Conjugated linoleic acid and obesity control: efficacy and mechanisms

2004

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Obesity is associated with high blood cholesterol and high risk for developing diabetes and cardiovascular disease. Therefore, management of body weight and obesity are increasingly considered as an important approach to maintaining healthy cholesterol profiles and reducing cardiovascular risk. The present review addresses the effects of conjugated linoleic acid (CLA) on fat deposition, body weight and composition, safety, as well as mechanisms involved in animals and humans. Animal studies have shown promising effects of CLA on body weight and fat deposition. The majority of the animal studies have been conducted using CLA mixtures that contained approximately equal amounts of trans-10, cis-12 (t10c12) and cis-9, trans-11 (c9t11) isomers. Results of a few studies in mice fed CLA mixtures with different ratios of c9t11 and t10c12 isomers have indicated that the t10c12 isomer CLA may be the active form of CLA affecting weight gain and fat deposition. Inductions of leptin reduction and insulin resistance are the adverse effects of CLA observed in only mice. In pigs, the effects of CLA on weight gain and fat deposition are inconsistent, and no adverse effects of CLA have been reported. A number of human studies suggest that CLA supplementation has no effect on body weight and insulin sensitivity. Although it is suggested that the t10c12 CLA is the antiadipogenic isomer of CLA in humans, the effects of CLA on fat deposition are marginal and more equivocal as compared to results observed in animal studies. Mechanisms through which CLA reduces body weight and fat deposition remain to be fully understood. Proposed antiobesity mechanisms of CLA include decreased energy/food intake and increased energy expenditure, decreased preadipocyte differentiation and proliferation, decreased lipogenesis, and increased lipolysis and fat oxidation. In summary, CLA reduces weight gain and fat deposition in rodents, while produces less significant and inconsistent effects on body weight and composition in pigs and humans. New studies are required to examine isomer-specific effects and mechanisms of CLA in animals and humans using purified individual CLA isomers.

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Section: Introductionmentioning

confidence: 99%

“…CLA reduces fat deposition Dietary CLA decreases adiposity in different animal models (Table 2), including mice, [10][11][12][13]15,20,23 rats, 21,22,24 hamsters 14 and pigs. 16,32,35,36,39 It has been reported that diet supplementation with CLA at a level as low as 0.5% reduced fat deposition in both male and female ICR mice.…”

Section: Introductionmentioning

confidence: 99%

Conjugated linoleic acid and obesity control: efficacy and mechanisms

2004

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“…It has been reported that t10, c12 CLA is the most potent isomer in terms of potential to prevent cell proliferation and induce apoptosis in cancer cells (Cho et al, 2005(Cho et al, , 2006Kim et al, 2002a; Lee et al, 2006b; Ochoa et al, 2004). t10, c12 CLA is also associated with decreased body fat and increased lean body mass in various animal species, such as mice (Park et al, 1997), chickens (Cherian et al, 2005), rats (Yamasaki et al, 2003), hamsters (Navarro et al, 2006), pigs (Ostrowska et al, 1999) and, to some extent, humans (Blankson et al, 2000;Mougios et al, 2001; Risérus et al, 2001;Smedman & Vessby, 2001;Thom et al, 2001). Commensal Bifidobacterium and Lactobacillus species from the mammalian gastrointestinal Abbreviations: CLA, conjugated linoleic acid; FAME, fatty acid methyl ester; PAI, Propionibacterium acnes isomerase.…”

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confidence: 99%

Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

Rosberg-Cody

Stanton

O’Mahony³

et al. 2011

Microbiology

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We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P,0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat. INTRODUCTIONEvidence is emerging to support the concept that the enteric microbiota can exert profound effects on human health and disease, involving complex host-bacteria interactions that are as yet poorly understood. The gut microbiota is important to the host with regard to metabolic functions, providing nutrients and conferring an ability to resist bacterial infections (Marchesi & Shanahan, 2007;Wilks, 2007), as well as playing a dominant role in the education of the intestinal mucosal immune responses. Furthermore, the enteric microbiota has been shown to exert effects on disease processes outside the gut, including an impact on obesity and non-alcoholic fatty liver disease (Dumas et al., 2006;Marchesi & Shanahan, 2007).Conjugated linoleic acid (CLA) is a beneficial bacterial metabolite formed via microbial isomerization of linoleic acid. CLA is a collective term describing different isomers of linoleic acid with conjugated double bonds, the cis-9, trans-11 (c9, t11) CLA isomer being the most abundant form and the t10, c12 CLA isomer accounting for~1 % of total milk fat CLA (Jensen, 2002). It has been reported that t10, c12 CLA is the most potent isomer in terms of potential to prevent cell proliferation and induce apoptosis in cancer cells (Cho et al., 2005(Cho et al., , 2006Kim et al., 2002a; Lee et al., 2006b; Ochoa et al., 2004). t10, c12 CLA is also associated with decreased body fat and increased lean body mass in various animal...

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“…Nesse sentido, é esperado que o efeito redutor de gordura corporal do CLA tenha relação com os níveis de leptina 124 . De fato, pesquisadores objetivando estudar essa relação demonstram que o consumo de CLA reduz os níveis sanguíneos de leptina em ratos OLETF 119 e ratos Sprague-Dawley 125 . Esses efeitos, contudo, não foram observados por Corino et al 71 e Medina et al 126 , cujos trabalhos foram realizados com humanos e coelhos, respectivamente.…”

Section: Cla Metabolismo De Glicose E Lipídeos E Composição Corporalunclassified

Os efeitos do ácido linoléico conjugado no metabolismo animal: avanço das pesquisas e perspectivas para o futuro

2008

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Modulation of body fat and serum leptin levels by dietary conjugated linoleic acid in Sprague-Dawley rats fed various fat-level diets

Cited by 87 publications

References 26 publications

Conjugated linoleic acid and obesity control: efficacy and mechanisms

Conjugated linoleic acid and obesity control: efficacy and mechanisms

Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

Os efeitos do ácido linoléico conjugado no metabolismo animal: avanço das pesquisas e perspectivas para o futuro

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