Modulation of biological processes in the nucleus by delivery of DNA oligonucleotides conjugated with gold nanoparticles

Kim, Dong-Wook; Kim, Jae Hong; Park, Soo‐Jin; Yeom, Ji-Hyun; Go, Hayoung; Kim, Sudeok; Han, Min Su; Lee, Kangseok; Bae, Jang–Ho

doi:10.1016/j.biomaterials.2010.11.074

Cited by 33 publications

(26 citation statements)

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“…Examples include phosphorothioate, 2-O-methoxyethyl, 2-O-methyl, locked nucleic acid, morpholina, and peptide nucleic acid. [58][59][60][61] However, it is not known if these chemical modifications can be introduced into the NB without hindering its function as a PCR primer.…”

Section: Discussionmentioning

confidence: 99%

Nanobarcoding: detecting nanoparticles in biological samples using in situ polymerase chain reaction

Eustaquio¹,

Leary²

2012

IJN

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Background: Determination of the fate of nanoparticles (NPs) in a biological system, or NP biodistribution, is critical in evaluating an NP formulation for nanomedicine. Current methods to determine NP biodistribution are greatly inadequate, due to their limited detection thresholds. Herein, proof of concept of a novel method for improved NP detection based on in situ polymerase chain reaction (ISPCR), coined "nanobarcoding," is demonstrated. Methods: Nanobarcoded superparamagnetic iron oxide nanoparticles (NB-SPIONs) were characterized by dynamic light scattering, zeta potential, and hyperspectral imaging measurements. Cellular uptake of Cy5-labeled NB-SPIONs (Cy5-NB-SPIONs) was imaged by confocal microscopy. The feasibility of the nanobarcoding method was first validated by solution-phase PCR and "pseudo"-ISPCR before implementation in the model in vitro system of HeLa human cervical adenocarcinoma cells, a cell line commonly used for ISPCR-mediated detection of human papilloma virus (HPV). Results: Dynamic light-scattering measurements showed that NB conjugation stabilized SPION size in different dispersion media compared to that of its precursor, carboxylated SPIONs (COOHSPIONs), while the zeta potential became more positive after NB conjugation. Hyperspectral imaging confirmed NB conjugation and showed that the NB completely covered the SPION surface. Solution-phase PCR and pseudo-ISPCR showed that the expected amplicons were exclusively generated from the NB-SPIONs in a dose-dependent manner. Although confocal microscopy revealed minimal cellular uptake of Cy5-NB-SPIONs at 50 nM over 24 hours in individual cells, ISPCR detected definitive NB-SPION signals inside HeLa cells over large sample areas. Conclusion: Proof of concept of the nanobarcoding method has been demonstrated in in vitro systems, but the technique needs further development before its widespread use as a standardized assay.

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Section: Discussionmentioning

confidence: 99%

Nanobarcoding: detecting nanoparticles in biological samples using in situ polymerase chain reaction

Eustaquio¹,

Leary²

2012

IJN

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“…Other metallic NPs functionalization methods take place after an incubation period and through electrostatic interactions, making use of the cationic surface of some NPs and the anionic character of the target biomolecule, for example siRNA or DNA ,. Another possible strategy is to employ thiolated siRNA fragments that favor adsorption onto the noble metal's surface .…”

Section: Synthetic Pathways For Drug Loadingmentioning

confidence: 99%

“…Cationic peptides such as TAT or PEI can be employed to introduce proteins, active principles, RNA or DNA in the tumor cell . The cationic character of the surface of the NPs of noble metals like gold favours the functionalization in their surface of DNA or RNA by electrostatic interactions ,,. Examples of these uses include magnetic NPs with DNA or gold NPs with siRNA containing the cationic peptide PEI, which, although toxic, facilitates endosome aperture and the entrance of the RNA that will silence gene expression inside the cell ,…”

Section: Action Mechanisms In Vivomentioning

confidence: 99%

Exploring Factors for the Design of Nanoparticles as Drug Delivery Vectors

et al. 2018

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To achieve optimal results when employing nanoparticles in biomedical fields, choosing the right type of nanoparticle and determining the correct procedure for drug loading are key factors. Each type of nanoparticle presents a determined set of characteristics that are, in some cases, unique. In general, their surface charge, geometry or hydrophilic character may be limiting factors, depending on what their intended application is. Once synthesized, additional factors, such as their interaction with biological systems and liberation mechanisms into the target cells, also need to be taken into account. Multiple advantages arise from the use of nanoparticles, such as the capability to solubilize hydrophobic compounds and an increased bioavailability. Those advantages justify the extensive and delicate study that should be undertaken in order to use them as drug delivery agents. One of the most important factors for the design of a drug delivery system with nanoparticles is achieving a high drug-to-nanoparticle ratio. In this Minireview, all of these key factors, both physicochemical and biological, are described, and special emphasis is placed on loading methods employed to introduce drugs into nanoparticles.

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“…Tumor sections were prepared and immunostained as previously described [29]. Polyclonal anti-BIM antibody (1:100) (Santa Cruz Biotechnology, USA) was diluted in an antibody diluent (Dako, USA).…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Gold nanoparticle–DNA aptamer composites as a universal carrier for in vivo delivery of biologically functional proteins

Ryou

Yeom

Kang

et al. 2014

Journal of Controlled Release

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Modulation of biological processes in the nucleus by delivery of DNA oligonucleotides conjugated with gold nanoparticles

Cited by 33 publications

References 50 publications

Nanobarcoding: detecting nanoparticles in biological samples using in situ polymerase chain reaction

Nanobarcoding: detecting nanoparticles in biological samples using in situ polymerase chain reaction

Exploring Factors for the Design of Nanoparticles as Drug Delivery Vectors

Gold nanoparticle–DNA aptamer composites as a universal carrier for in vivo delivery of biologically functional proteins

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