Modulation of amylose content by structure‐based modification of Os<scp>GBSS</scp>1 activity in rice (<i><scp>O</scp>ryza sativa</i> L.)

Liu, Qiaoquan; Wang, Wei; Cai, Xin-Zhong

doi:10.1111/pbi.12228

Cited by 48 publications

(50 citation statements)

References 42 publications

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“…Waxy was the most likely mutated gene responsible for relative low AC as the evidence that the levels of Wx RNA expression and the activity of the OsGBSS1 protein are positively correlated with AC (Liu et al. ). However, mutation of other starch synthesis genes and starch synthesis‐related genes might play some role in low AC without any sequence analysis of these genes in the 12 glutinous rice mutants.…”

Section: Discussionmentioning

confidence: 99%

“…These results are similar to the SNP in Wx in and Wx op genotype (Mikami et al 2008) or the point mutation found in japonica rice lines by , resulting in non-synonymous mutation in wx and influencing amylose content. Waxy was the most likely mutated gene responsible for relative low AC as the evidence that the levels of Wx RNA expression and the activity of the OsGBSS1 protein are positively correlated with AC (Liu et al 2014). However, mutation of other starch synthesis genes and starch synthesisrelated genes might play some role in low AC without any sequence analysis of these genes in the 12 glutinous rice mutants.…”

Section: Discussionmentioning

confidence: 99%

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Novel Wx alleles induced by chemical mutagens in rice, Oryza sativa L.

et al. 2017

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From mutant pools of two Taiwanese elite japonica cultivars, Tainung 67 and Taikeng 8, we identified 13 mutant lines possessing opaque endosperm with relatively low amylose contents (AC) ranging from 1.5% to 7.1%. Because of different AC, paste viscosities of these 13 mutant lines differed, as revealed by palatability and physicochemical properties. The mutated gene conferring opaque endosperm was isolated from the F2 population of one mutant line, WY1× indica cv. ‘Taichung Sen 17’, by positional cloning, revealing a G3018→A3018 substitution at exon 9 of Waxy leading to a non‐synonymous mutation from alanine to valine. Two additional alleles were identified from the other 12 mutant lines, for which single‐nucleotide substitutions G2708 → A2708 and G3029 → A3029 occurred in exons 8 and 9, leading to non‐synonymous mutations from arginine to histidine and glutamic acid to lysine, respectively. The three novel wx alleles had different effects on grain quality, specifically on eating and cooking quality, and could be applied in rice breeding programmes to develop new low AC varieties by marker‐assisted selection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Novel Wx alleles induced by chemical mutagens in rice, Oryza sativa L.

et al. 2017

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“…The NC membrane was incubated in blocking buffer adding antibodies and was detected with ECL Plus reagent. Antibodies against GBSSI, SSI, BEI, BEIIa and BEIIb were described previously [46]. …”

Section: Methodsmentioning

confidence: 99%

A Novel Mutation of OsPPDKB, Encoding Pyruvate Orthophosphate Dikinase, Affects Metabolism and Structure of Starch in the Rice Endosperm

Zhang

Zhao

Lin

et al. 2018

IJMS

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Starch, as a main energy storage substance, plays an important role in plant growth and human life. Despite the fact that several enzymes and regulators involved in starch biosynthesis have been identified, the regulating mechanism of starch synthesis is still unclear. In this study, we isolated a rice floury endosperm mutant M14 from a mutant pool induced by 60Co. Both total starch content and amylose content in M14 seeds significantly decreased, and starch thermal and pasting properties changed. Compound starch granules were defected in the floury endosperm of M14 seeds. Map-based cloning and a complementation test showed that the floury endosperm phenotype was determined by a gene of OsPPDKB, which encodes pyruvate orthophosphate dikinase (PPDK, EC 2.7.9.1). Subcellular localization analysis demonstrated that PPDK was localized in chloroplast and cytoplasm, the chOsPPDKB highly expressed in leaf and leaf sheath, and the cyOsPPDKB constitutively expressed with a high expression in developing endosperm. Moreover, the expression of starch synthesis-related genes was also obviously altered in M14 developing endosperm. The above results indicated that PPDK played an important role in starch metabolism and structure in rice endosperm.

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“…AC, as an important physicochemical property for rice eating and cooking quality, is mainly determined by GBSSI (Liu et al ., ; Sano, ; Wang et al ., ). Previous data showed that the Wx gene encoding GBSSI was down‐regulated at HT, which led to a considerable reduction in AC (Lin et al ., ; Yamakawa et al ., ; Zhang et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Suppression of OsMADS7 in rice endosperm stabilizes amylose content under high temperature stress

Zhang

Feng

et al. 2017

Plant Biotechnology Journal

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SummaryHigh temperature significantly alters the amylose content of rice, resulting in mature grains with poor eating quality. However, only few genes and/or quantitative trait loci involved in this process have been isolated and the molecular mechanisms of this effect remain unclear. Here, we describe a floral organ identity gene, OsMADS7, involved in stabilizing rice amylose content at high temperature. OsMADS7 is greatly induced by high temperature at the early filling stage. Constitutive suppression of OsMADS7 stabilizes amylose content under high temperature stress but results in low spikelet fertility. However, rice plants with both stable amylose content at high temperature and normal spikelet fertility can be obtained by specifically suppressing OsMADS7 in endosperm. GBSSI is the major enzyme responsible for amylose biosynthesis. A low filling rate and high expression of GBSSI were detected in OsMADS7 RNAi plants at high temperature, which may be correlated with stabilized amylose content in these transgenic seeds under high temperature. Thus, specific suppression of OsMADS7 in endosperm could improve the stability of rice amylose content at high temperature, and such transgenic materials may be a valuable genetic resource for breeding rice with elite thermal resilience.

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Modulation of amylose content by structure‐based modification of OsGBSS1 activity in rice (Oryza sativa L.)

Cited by 48 publications

References 42 publications

Novel Wx alleles induced by chemical mutagens in rice, Oryza sativa L.

Novel Wx alleles induced by chemical mutagens in rice, Oryza sativa L.

A Novel Mutation of OsPPDKB, Encoding Pyruvate Orthophosphate Dikinase, Affects Metabolism and Structure of Starch in the Rice Endosperm

Suppression of OsMADS7 in rice endosperm stabilizes amylose content under high temperature stress

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