Modulation of Actinomyces viscosus colonization of mouse teeth in vivo by immunization with fimbrial adhesins

Crawford, P. Cynda; Clark, William

doi:10.1128/iai.54.2.516-521.1986

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…Determination of the humoral immune response against fimbriae was included in the study because B. gingivalis fimbriae are well characterized biochemically (55,56), and are considered important for the adhesion of B. gingivalis to oral tissues (41). Furthermore, they elicit considerable immune response in human gingiva (40), and colonization of Actinomyees viscosus in mice has been reduced considerably by immunization with fimbrial adhesins (10). Consequently, B. gingivalis fimbrial antigens may be relevant alternatives to whole cells as immunogen in future immunization studies in the gnotobiotic rat model as well as in higher species, including humans.…”

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confidence: 99%

Periodontal bone level and gingival proteinase activity in gnotobiotic rats immunized with Bacteroides gingivalis

Klausen

Evans

Ramamurthy

et al. 1991

Oral Microbiology and Immunology

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Bacteroides gingivalis is associated with various forms of periodontal disease. To assess the role of the immune response in modulating B. gingivalis-associated periodontal disease, the effect of immunization of B. gingivalis-induced periodontal bone loss was evaluated in gnotobiotic rats. Male Sprague-Dawley rats immunized with various doses of whole cells or sham-immunized with incomplete Freund's adjuvant were monoinfected with B. gingivalis in carboxymethylcellulose by gavage. Two additional groups served as either sham-immunized or untreated germ-free controls. Forty-two days after infection, all rats were killed, periodontal bone level was assessed morphometrically and radiographically, and gingival proteinase (mammalian collagenase and acid cathepsin) activity was assessed biochemically. B. gingivalis was present in oral samples from all monoinfected rats, and no contaminating bacteria were detected in any oral or fecal sample. Animals immunized with B. gingivalis cells had elevated serum and saliva antibodies to whole cells and partially purified fimbriae from B. gingivalis. Infected sham-immunized rats had significantly more periodontal bone loss than noninfected controls, whereas the periodontal bone level in infected rats immunized with 10(10) B. gingivalis cells was similar to that of the noninfected controls. The activities of gingival collagenase and cathepsin B and L were high in sham-immunized infected rats and low in all other animal groups. In conclusion, it is possible to reduce B. gingivalis-induced periodontal tissue loss in gnotobiotic rats by immunization.

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confidence: 99%

Periodontal bone level and gingival proteinase activity in gnotobiotic rats immunized with Bacteroides gingivalis

Klausen

Evans

Ramamurthy

et al. 1991

Oral Microbiology and Immunology

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“…As this bacterium is part of the commensal oral flora, continuous swallowing of these bacteria could stimulate an immune response in the lower gastrointestinal tract. Thus, it can be speculated that the induction of oral tolerance to periodontopathic bacteria may contribute to the immune imbalance of the inflamed gingival (Sosroseno and Herminajeng, 1995; Gemmell et al , 2002) and the local immune response that may also be induced (Crawford and Clark, 1986; Mahanonda et al , 2002; Han et al , 2003) would prevent the adherence of these microorganisms on the tooth and mucosal surface.…”

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confidence: 99%