Modulation by Retinoic Acid of 1,25-Dihydroxyvitamin D<sub>3</sub>Effects on Alkaline Phosphatase Activity and Parathyroid Hormone Responsiveness in an Osteoblast-Like Osteosarcoma Cell Line

Grigoriadis, Agamemnon E.; Petkovich, P. Martin; Rosenthal, Esther; Heersche, J. N. M.

doi:10.1210/endo-119-2-932

Cited by 46 publications

(21 citation statements)

References 32 publications

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“…The effective concentration range of calcitriol for FBPase induction is approximately consistent with those for the inductions of lysozyme in HL-60 [21] and M-I [22] cells, of alkaline phosphatase in ROS17/2 cells [23] and of nitroblue-tetrazolium-reducing capability in U937 cells [24]. The induced enzyme activity is strongly inhibited by AMP, a specific allosteric inhibitor of FBPase, and precipitated with anti-(rat liver FBPase) serum.…”

Section: Discussionsupporting

confidence: 59%

Induction and Turnover of Fructose 1,6‐Bisphosphatase in HL‐60 Leukemia Cells by Calcitriol

Mizunuma

Tashima

1994

European Journal of Biochemistry

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Fructose 1,6-bisphosphatase mRNA and enzyme activity in HL-60 cells were rapidly and markedly induced by calcitriol (formerly known as la,25-dihydroxyvitamin D3). The activity reached 70-80 times the basal level after 96 h. The enzyme activity in the cells incubated for 96 h with calcitriol decreased immediately after its withdrawal but after a 24-h incubation the activity in the cells continued to increase slightly and then decreased slowly. Calcitriol increased the enzyme activity dose-dependently with maximal stimulation at 10 nM and half-maximal at 2.1 nM. The rate of synthesis of fructose 1,6-bisphosphatase almost paralleled the increase in inRNA level during treatment with calcitriol. When calcitriol was removed from media after incubation for either 24 h or 96 h, fructose-1;6-bisphosphatase mRNA and fructose-l,6-bisphosphatase synthesis decreased rapidly to the basal level. The enzyme was only slightly degraded in the cells incubated with calcitriol for 24 h followed by the subsequent culture without calcitriol but it was degraded with a half-life estimated to be approximately 64 h in the same cells followed by culturing with calcitriol. In the cells incubated for 96 h, the same degradation rate (i.e. half-life =64 h) was observed irrespective of the following culture with or without calcitriol. Calcitriol did not affect the degradation rate of total soluble proteins.Fructose 1,6-bisphosphatase (FBPase) is one of the ratelimiting enzymes in hepatic gluconeogenesis. The in vivo level of FBPase is under nutritional and hormonal controls. The intracellular concentration of the enzyme in the liver increases under gluconeogenic conditions including fasting, a high-protein diet and alloxan diabetes [l]. The enzyme concentration is also increased by the injection of glucagon, glucocorticoids and thyroxine [2]. El-Maghrabi et al. showed that FBPase mRNA was increased in livers from adult diabetic rats and was reduced to control levels after insulin treatment [3]. Recently, they observed the accumulation of FBPase mRNA in primary hepatocyte cultures incubated with cAMP and showed the presence of CAMP-responsive elements in the 5'-flanking region of the rat FBPase gene [4].FBPase is present in fetal liver and increases exponentially during fetal development [ 5 ] . In organ culture from fetal mouse liver, the activity of FBPase is remarkably elevated in the presence of dibutyryl cAMP [6, 71. FBPase activity, however, increases during the culture even in the absence of an inducer, although at lower rates [6]. The increase in intracellular FBPase concentration, especially in fetal development, might also be regulated by another factor (s) in addition to elevation of CAMP.

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Section: Discussionsupporting

confidence: 59%

Induction and Turnover of Fructose 1,6‐Bisphosphatase in HL‐60 Leukemia Cells by Calcitriol

Mizunuma

Tashima

1994

European Journal of Biochemistry

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“…At 2-day intervals, medium was replaced with fresh medium. Alkaline phosphatase (AP) activity was determined on days 0, 3 and 6 according to established methods (Grigoriadis et al 1986). Enzyme activity is expressed as nmol p-nitrophenol formed/min per µg protein.…”

Section: Methodsmentioning

confidence: 99%

Interaction between insulin-like growth factor-I and insulin-like growth factor-binding proteins in TC-1 stromal cells

Grellier

Féliers

Yee

et al. 1996

Journal of Endocrinology

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IGF-I and -II play an important role in regulating bone formation. Bone marrow stromal cells, particularly those with osteoblast-like features, may act in concert with osteoblasts to increase IGF-I and -II levels in the bone microenvironment. Local bioavailability of IGFs, however, is modulated by IGF binding proteins (IGFBPs). We have previously demonstrated that murine TC-1 stromal cells constitutively secrete IGF-I and IGFBPs. In the present study, we determined the phenotype of these cells and used them as a model to explore the effect of IGFBPs on IGF-I-induced mitogenesis. The effect of IGF-I on IGFBPs expressed by TC-1 was also determined. When grown under conditions that promote osteogenic differentiation, TC-1 cells showed high alkaline phosphatase activity and mRNA levels, weakly expressed osteocalcin mRNA, and formed mineralized bone-like nodules. TC-1 cells expressed IGF-I and IGF-II mRNAs, while other stromal phenotypes preferentially expressed IGF-I. IGF-I stimulated TC-1 DNA synthesis in a dose-dependent manner and this effect was inhibited by recombinant IGFBP-1 and -4. Since IGF-I may regulate IGFBP production, the effect of IGF-I on IGFBPs expressed by TC-1 cells was determined. IGF-I increased the abundance of IGFBP-3, -4 and -5 in TC-1 conditioned medium; this correlated with induction of IGFBP-3 mRNA, but not with that of IGFBP-4 or -5 mRNAs. The findings demonstrate that most stromal cells express IGF-I which may act in an autocrine and/or paracrine fashion. The local effects of IGF-I, however, may be blocked by IGFBP-1 or -4. IGF-I regulates the relative abundance of IGFBPs in stromal cells which, in turn, may influence IGF-I-mediated effects on bone remodeling.

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“…At different time points cells were rinsed with PBS, sonicated, collected with a rubber policeman, and centrifuged to remove cell debris. Alkaline phosphatase activity was determined by the addition of p-nitrophenyl phosphate in 0.1 M glycine, 1 mM MgCl 2 , pH 10.5, at 37 • C for 20 min [29,30]. The product, p-nitrophenol, was measured as absorbance at 405 nm; p-nitrophenol produced was determined from standard curve for each assay and normalized to 10,000 cells.…”

Section: Alkaline Phosphatase Activitymentioning

confidence: 99%

Comparison of Fetal and Adult Marrow Stromal Cells in Osteogenesis with and without Glucocorticoids

Chang

Blair

Zhao

et al. 2006

Connective Tissue Research

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To better understand the potential use of fetal marrow stromal cells (MSCs) in bone tissue engineering, we compared the ability of these cells with those of adult MSCs with respect to osteoblasts differentiation in the presence or absence of glucocorticoids. Cells were grown for 3-4 weeks in basal medium or supplemented with 100 nM dexamethasone (DEX, a synthetic glucocorticoid analog) or with 50 microM L-ascorbate and 10 mM glycerol-2-phosphate (AS+GP) or with AS+GP+DEX. At various time points in culture, the following parameters were compared between fetal and adult MSCs: cell morphology, cell proliferation, alkaline phosphatase activity, calcium (45Ca) uptake, von Kossa staining, and glucocorticoids receptor expression were analyzed. Compared with adult MSCs, fetal cells showed a less dramatic change to cuboidal morphology in DEX-containing media. Fetal MSCs in all media conditions showed higher proliferation rates and lower alkaline phosphatase activities (p < 0.001) than adult cells. Both fetal and adult MSCs responded similarly in DEX-containing media with respect to suppressing cell proliferation, stimulating alkaline phosphatase activity, and consistently accumulating calcium (usually higher in fetal cells) with subsequent formation of mineralized matrix when compared with cells cultured in AS+GP. Our findings further implicate the requirement of glucocorticoids in osteogenesis. In conclusion, compared with adult MSCs, fetal cells showed greater ability in sustaining cell proliferation and calcium uptake suggesting that they may be useful for bone tissue repair.

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Modulation by Retinoic Acid of 1,25-Dihydroxyvitamin D₃Effects on Alkaline Phosphatase Activity and Parathyroid Hormone Responsiveness in an Osteoblast-Like Osteosarcoma Cell Line

Cited by 46 publications

References 32 publications

Induction and Turnover of Fructose 1,6‐Bisphosphatase in HL‐60 Leukemia Cells by Calcitriol

Induction and Turnover of Fructose 1,6‐Bisphosphatase in HL‐60 Leukemia Cells by Calcitriol

Interaction between insulin-like growth factor-I and insulin-like growth factor-binding proteins in TC-1 stromal cells

Comparison of Fetal and Adult Marrow Stromal Cells in Osteogenesis with and without Glucocorticoids

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Modulation by Retinoic Acid of 1,25-Dihydroxyvitamin D3Effects on Alkaline Phosphatase Activity and Parathyroid Hormone Responsiveness in an Osteoblast-Like Osteosarcoma Cell Line

Cited by 46 publications

References 32 publications

Induction and Turnover of Fructose 1,6‐Bisphosphatase in HL‐60 Leukemia Cells by Calcitriol

Induction and Turnover of Fructose 1,6‐Bisphosphatase in HL‐60 Leukemia Cells by Calcitriol

Interaction between insulin-like growth factor-I and insulin-like growth factor-binding proteins in TC-1 stromal cells

Comparison of Fetal and Adult Marrow Stromal Cells in Osteogenesis with and without Glucocorticoids

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Modulation by Retinoic Acid of 1,25-Dihydroxyvitamin D₃Effects on Alkaline Phosphatase Activity and Parathyroid Hormone Responsiveness in an Osteoblast-Like Osteosarcoma Cell Line