1986
DOI: 10.1210/endo-119-2-932
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Modulation by Retinoic Acid of 1,25-Dihydroxyvitamin D3Effects on Alkaline Phosphatase Activity and Parathyroid Hormone Responsiveness in an Osteoblast-Like Osteosarcoma Cell Line

Abstract: Based on the finding that retinoic acid (RA) increases 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] receptor number in ROS 17/2 cells, we investigated the effects of RA on the ability of 1,25-(OH)2D3 to regulate alkaline phosphatase activity and PTH-responsive adenylate cyclase in these cells. A maximally effective dose of 1,25-(OH)2D3 (10(-8) M) caused a 75-80% increase in alkaline phosphatase activity and an approximately 70-75% attenuation of the cAMP response to PTH, while RA (10(-6) M) decreased alkaline phosp… Show more

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Cited by 46 publications
(21 citation statements)
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“…The effective concentration range of calcitriol for FBPase induction is approximately consistent with those for the inductions of lysozyme in HL-60 [21] and M-I [22] cells, of alkaline phosphatase in ROS17/2 cells [23] and of nitroblue-tetrazolium-reducing capability in U937 cells [24]. The induced enzyme activity is strongly inhibited by AMP, a specific allosteric inhibitor of FBPase, and precipitated with anti-(rat liver FBPase) serum.…”
Section: Discussionsupporting
confidence: 59%
“…The effective concentration range of calcitriol for FBPase induction is approximately consistent with those for the inductions of lysozyme in HL-60 [21] and M-I [22] cells, of alkaline phosphatase in ROS17/2 cells [23] and of nitroblue-tetrazolium-reducing capability in U937 cells [24]. The induced enzyme activity is strongly inhibited by AMP, a specific allosteric inhibitor of FBPase, and precipitated with anti-(rat liver FBPase) serum.…”
Section: Discussionsupporting
confidence: 59%
“…At 2-day intervals, medium was replaced with fresh medium. Alkaline phosphatase (AP) activity was determined on days 0, 3 and 6 according to established methods (Grigoriadis et al 1986). Enzyme activity is expressed as nmol p-nitrophenol formed/min per µg protein.…”
Section: Methodsmentioning
confidence: 99%
“…At different time points cells were rinsed with PBS, sonicated, collected with a rubber policeman, and centrifuged to remove cell debris. Alkaline phosphatase activity was determined by the addition of p-nitrophenyl phosphate in 0.1 M glycine, 1 mM MgCl 2 , pH 10.5, at 37 • C for 20 min [29,30]. The product, p-nitrophenol, was measured as absorbance at 405 nm; p-nitrophenol produced was determined from standard curve for each assay and normalized to 10,000 cells.…”
Section: Alkaline Phosphatase Activitymentioning
confidence: 99%