Modulating Role of Bitter Leaf on Spermatogenic and Steroidogenesis Functions of the Rat Testis

Saalu, L.C.; Akunna, G.G.; Ajayi, J.O.

doi:10.3923/ajbmb.2013.314.321

Cited by 16 publications

(14 citation statements)

References 8 publications

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“…In the present investigation the observed increased the motile spermatozoa and decreased immotile sperm in groups A and B. Similar results were observed with rats treated with bitter leaves [ 19 ] and Telfairia occidentalis leaves extract [ 20 ]. They suggested that increase in motility is due to increase of glucose metabolism resulting in production of pyruvate that is essential for the activity and survival of sperm cells.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Aqueous Leaf Extract ofCardiospermum halicacabum(L.) on Fertility of Male Rats

Peiris

Dhanushka²,

Jayathilake

2015

BioMed Research International

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Treatment with 100 mg/kg and 200 mg/kg body weight of aqueous leaf extract (ALE) of Cardiospermum halicacabum for 30 days produced a significant dose dependent increase in the sperm counts and sperm motility in both caput and cauda regions. Further, significant increase in serum testosterone level was evident at all applied doses. However, no significant changes in the weight of sex organs were observed. Aqueous leaf extract also increased the number of females impregnated, number of implantations, and number of viable fetuses while decreasing the total number of resorption sites in the pregnant females. However, the total cholesterol level in the serum remained unchanged and there were no records on renotoxicity; nevertheless ALE exhibited a hepatoprotective effect. It was concluded that aqueous leaf extract of Cardiospermum halicacabum enhanced sperm concentration, motility, and testosterone, leading to positive results in fertility.

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Section: Discussionsupporting

confidence: 91%

Evaluation of Aqueous Leaf Extract ofCardiospermum halicacabum(L.) on Fertility of Male Rats

Peiris

Dhanushka²,

Jayathilake

2015

BioMed Research International

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“…In our findings, there were elevations in serum testosterone levels, follicle stimulating hormone and luteinizing hormone of the experimental groups treated with Solanum nigrum, which showed the positive effects of the Solanum nigrum extract in Sprague-Dawley rats. It has been reported that treatment with antioxidants enhances steroidogenesis by improving the primary effects of the leydig cells, endocrine function along with increased circulating testosterone secretions and stimulation of spermatogenesis ( Saalu et al, 2013 ; Prasad & Rajalakshmi, 1989 ). Hematological parameters showed that the hemoglobin (Hb) count increased significantly in the 100mg/kg and 500mg/kg body weight groups but there was significant decrease in Hb count of the group that received 300mg/kg per body weight.…”

Section: Discussionmentioning

confidence: 99%

“…Blood sample was collected throught cardiac puncture for hormonal assay. The hormone assay (testosterone, follicule stimulating hormone and lutenizing hormone) was carried out using the immunoassay method as discribed by Saalu et al (2013) and Yakubu et al . (2008) .…”

Section: Methodsmentioning

confidence: 99%

Histomorphology, Sperm Quality and Hormonal Profile in Adult Male Sprague-Dawley Rats following administration of aqueous crude Extract of Solanum nigrum by gastric gavage

Adelakun

Ogunlade

Olawuyi

et al. 2018

JBRA Assisted Reproduction

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ObjectiveThe present study focused on histomorphology, sperm quality, hormonal profile and hematological parameters in adult male Sprague-Dawley rats following the administration of aqueous crude extract of Solanum nigrum by gastric gavage.MethodsFourty healthy male adult (12-14 weeks old) Sprague-Dawley rats weighing 200-220g were randomly divided into four groups (A,B,C and D) of ten (n=10) rats each. Group A which served as control were given distilled water 2ml/kg b.wt each, daily for 28 days. Group B, C and D rats were administered 100, 300 and 500mg/kg b.wt each daily respectively for 28 days. The extract was saved with LD50 >5000mg/Kg. Sperm counts, percentage motility, morphology and percentage live sperm, hormonal profile and hematological parameter were quantified; testis, epididymal and general body weights were measured using a weighing scale. The extract was administered once daily for six days within a week via oral gavage. After the last administration, all rats were sacrificed by cervical dislocation, the testis were harvested and fixed in Bouin‘s fluid for histology processing.ResultsOur results revealed an increase in sperm counts, percentage of motility, morphology and percentage of live sperm, blood level of follicle stimulating hormone, Luteinizing hormone and testosterone, hematological parameters, testis, epididymal and general body weights across the groups in a dose-depentant manner. The testis histoarchtecture showed normal cellular composition in their germinal epithelium, with sperm cells in the lumen and a normal interstitium.ConclusionThis experiment revealed that aqueous extract of Solanum nigrum bears profertility properties which may be beneficial to those who consume it.

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“…Increased serum hormone level suggests the existence of a modulating effect of Senecio biafrae extract in rats. It has been shown that treatment with antioxidants improves steroidogenesis by enhancing the primary effect of the endocrine function of Leydig cells along with increased circulatory testosterone and stimulation of spermatogenesis ( Saalu et al ., 2013 ). Reductions in testosterone, FSH, and LH by tetracycline might result from tetracycline reaching the blood-testis barrier and gaining access to the germ cells in the seminiferous tubules, as previously described in the literature ( Dixon & Lee, 1973 ).…”

Section: Discussionmentioning

confidence: 99%

Senecio biafrae defeated Tetracycline-Induced Testicular Toxicity in Adult Male Sprague Dawley Rats

Adelakun

Omotoso

Aniah

et al. 2018

JBRA Assisted Reproduction

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ObjectiveThe current study focused on the pro-fertility potential of Senecio biafrae (Sb) extract and vitamin C in Male Sprague Dawley (SD) rats with tetracycline-induced infertility.MethodsA total of 36 male and 36 female adult SD rats were used for this investigation. The male rats randomly assigned to Group A (controls) were given normal saline 2ml/kg. Rats in Groups B, C, D, E, and F were respectively administered [30 mg/kg of body weight (bwt) of tetracycline], [30 mg/kg bwt of tetracycline + 50 mg/kg of vitamin C], [30 mg/kg bwt of tetracycline + 500 mg/kg bwt of Sb], [30 mg/kg bwt of tetracycline + 50 mg/kg of vitamin C + 500 mg/kg bwt of Sb], and [30 mg/kg bwt of tetracycline reversal] daily for 28 days via gastric gavage. Tested parameters included sperm parameters, hormonal profile, histology, and fertility test.ResultsSignificant (p<0.05) increases were seen in sperm quality, hormone profile, organ and body weights of the groups treated with vitamin C, Sb, and tetracycline. There was derangement in sperm quality, hormone profile, and organ and body weight of the animals in group B. Histoarchtecture of the testes showed normal cellular composition in the germinal epithelium with sperm cells in the lumen and normal interstitium in groups A, C, D, and E. Group F showed abnormal spermatogenesis and poor association of spermatogenic cells, however there was depletion in the seminiferous epithelium in the group treated with tetracycline.ConclusionSenecio biafrae defeated the deleterious effects of tetracycline on the male reproductive system of rats treated with the drug.

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Modulating Role of Bitter Leaf on Spermatogenic and Steroidogenesis Functions of the Rat Testis

Cited by 16 publications

References 8 publications

Evaluation of Aqueous Leaf Extract ofCardiospermum halicacabum(L.) on Fertility of Male Rats

Evaluation of Aqueous Leaf Extract ofCardiospermum halicacabum(L.) on Fertility of Male Rats

Histomorphology, Sperm Quality and Hormonal Profile in Adult Male Sprague-Dawley Rats following administration of aqueous crude Extract of Solanum nigrum by gastric gavage

Senecio biafrae defeated Tetracycline-Induced Testicular Toxicity in Adult Male Sprague Dawley Rats

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