Senecio biafrae defeated Tetracycline-Induced Testicular Toxicity in Adult Male Sprague Dawley Rats

Abstract

ObjectiveThe current study focused on the pro-fertility potential of Senecio biafrae (Sb) extract and vitamin C in Male Sprague Dawley (SD) rats with tetracycline-induced infertility.MethodsA total of 36 male and 36 female adult SD rats were used for this investigation. The male rats randomly assigned to Group A (controls) were given normal saline 2ml/kg. Rats in Groups B, C, D, E, and F were respectively administered [30 mg/kg of body weight (bwt) of tetracycline], [30 mg/kg bwt of tetracycline + 50 mg/kg of vitamin C], [30 mg/kg bwt of tetracycline + 500 mg/kg bwt of Sb], [30 mg/kg bwt of tetracycline + 50 mg/kg of vitamin C + 500 mg/kg bwt of Sb], and [30 mg/kg bwt of tetracycline reversal] daily for 28 days via gastric gavage. Tested parameters included sperm parameters, hormonal profile, histology, and fertility test.ResultsSignificant (p<0.05) increases were seen in sperm quality, hormone profile, organ and body weights of the groups treated with vitamin C, Sb, and tetracycline. There was derangement in sperm quality, hormone profile, and organ and body weight of the animals in group B. Histoarchtecture of the testes showed normal cellular composition in the germinal epithelium with sperm cells in the lumen and normal interstitium in groups A, C, D, and E. Group F showed abnormal spermatogenesis and poor association of spermatogenic cells, however there was depletion in the seminiferous epithelium in the group treated with tetracycline.ConclusionSenecio biafrae defeated the deleterious effects of tetracycline on the male reproductive system of rats treated with the drug.

d‐ribose‐l‐cysteine abrogates testicular maladaptive responses induced by polychlorinated bisphenol intoxication in rats via activation of the mTOR signaling pathway mediating inhibition of apoptosis, inflammation, and oxidonitrergic flux

“…The induction of inflammation and oxidative stress by PCBs has been well established. [ 15,17,41,42 ] More so, the therapeutic indication of DRLC supplementation on reproductive potential has been widely documented; [ 17,21–25 ] however, the current study seems to be the first to investigate the effects of DRLC on testicular maladaptive responses.…”

“…[ 18–22 ] Also, the potential of repropharmacological activities of DRLC for treatment of repro‐toxicant‐related repro‐pathologies has gained increasing interest. [ 18,21–25 ]…”

Therapeutic effects of aqueous extract of bioactive active component of Ageratum conyzoides on the ovarian-uterine and hypophysis-gonadal axis in rat with polycystic ovary syndrome: Histomorphometric evaluation and biochemical assessment

“…Metestrus (the third stage) is made up of a variety of cell types, with leukocytes predominating with a few nucleated and/or cornifield squamous epithelial cells thrown in for good measure. Diestrus (the fourth stage) is primarily made up of leukocytes [ 40 , 41 ].…”

Natural Products as Protective Agents for Male Fertility

“…Some drugs can also induce reproductive toxicity in males, such as the antibiotic tetracycline or cisplatin. Vitamin C co-administration with these drugs restored histopathological changes [69,70], sperm parameters (counts, viability, motility, and morphology) [69][70][71] and increased the weight of the testis and accessory sex organs [70,71], FSH, LH [69,70], testosterone [69][70][71], SOD, GSH, GST, and glucose-6-phosphate dehydrogenase (G6PD) levels [71] and decreased MDA levels [70,71].…”