Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions

Chaudhary, Amrita; Bag, Swarnendu; Mandal, Mousumi; Karri, Sri Phani Krishna; Barui, Ananya; Rajput, Monika; Banerjee, P.; Sheet, Debdoot; Chatterjee, Jyotirmoy

doi:10.1016/j.jep.2015.03.017

Cited by 11 publications

(9 citation statements)

References 40 publications

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“…Wound healing potential of chlorogenic acid is accredited to its ability to increase cellular proliferation and its antioxidant potential accelerating the process (Chen et al, 2013). Earlier studies have also reported that honey which exhibits presence of chlorogenic acid also illustrated wound healing potential through free radical scavenging activity promoting cell proliferation in the acquisition of concurrence approaching rapid wound healing (Chaudhary et al, 2015). Furthermore, tannins act by chelating free radicals, facilitating wound contraction, accelerating capillary vessel and fibroblast formation and by instigating keratinocyte proliferation thus hastening the process of wound healing (Prasad et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Joshi

Pandey

et al. 2016

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 99%

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Joshi

Pandey

et al. 2016

Journal of Ethnopharmacology

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“…Polyphenolics are naturally present in honey which reduces the ROS and assist wound healing especially for the diabetic one 37 , 38 . The Ayurvedic texts cited the goodness of Honey (Madhu) because of its sweetness, astringent, healing and anti-inflammatory potentials 39 , 40 . But very few studies have been tried to decipher the molecular mechanism behind the significance of this ancient therapeutic agent in wound healing specially for collagen modification and angiogenesis 38 , 41 .…”

Section: Discussionmentioning

confidence: 99%

Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis

Chaudhary

Bag

Banerjee³

et al. 2020

Journal of Traditional and Complementary Medicine

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Diabetic patients are frequently afflicted with impaired wound healing where linear progression of molecular and cellular events compromised. Despite of meaningful progress in diabetic treatment, management of diabetic chronic wounds is still challenging. Jamun ( Syzygium cumini ) honey may be a promising candidate for diabetic wound healing and need to explore in detail. So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing in in vitro wound (primary fibroblasts) model and in in vivo of diabetic mice (Streptozotocin induced) model. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration under honey interventions via scratch/migration assay, immuno-cytochemistry and western blot. We applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound re-epithelialization, sub-epithelial connective tissue modification and angiogenesis via histo-pathological and immuno-histochemical analysis. JH (0.1% v/v) dilution has notably improved wound closure, migration with concomitant α-SMA expressions in vitro . Topical application of JH in diabetic mice model showed significant (*p ≤ 0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. It also modulated vital angiogenic markers ( viz HIF-1α, VEGF, VEGF R–II) significantly (*p ≤ 0.05). All these observations depicted that JH promotes sequential stages of wound healing in diabetic mice model. The results of the present study established Jamun honey as good as Manuka honey considering wound closure, re-epithelialization, collagen deposition and pro-angiogenic potential.

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“…The authors conclude that presented evidence shows that presence of honey in alginate matrix improves molecular events associated with re-epithelization process (Barui et al, 2013). Chaudhary et al (2015) used honey of Indian origin, without specifying floral origin, on HaCaT cells and observed that a dilution of 0.1% of honey increased expression of p63, indicative of cell proliferation, which was associated with a wound closure more rapid, together with a cytoplasmic expression of E-cadherin and β-catenin suggesting migration of cells during wound closure.…”

Section: Effects Of Honey At the Molecular Level In The Process Of Rementioning

confidence: 88%

Molecular Mechanisms in the Process of Re-epithelization in Wound Healing and the Action of Honey in Keratinocytes

2020

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The treatment of chronic wounds has become a public health issue in recent years mainly due to comorbidities associated with an older population and bacterial resistance. Honey has emerged as an alternative treatment for chronic wounds but lack of knowledge of its mechanism of actionin the treated tissue and low quality of evidence in clinical triads has distanced the medical community from honey as a possible treatment. One of the main processes that is altered in chronic wounds is re-epithelialization mediated by keratinocytes, where proliferation and migration processes are altered. Markers of proliferation, migration and activation of keratinocytes, such as adhesion molecules, growth factors, membrane receptors, signal translating proteins, transcription factors, microRNAs, among others are deregulated in this process. In general, honeys from different floral origins have a positive effect on markers of proliferation and migration in keratinocytes. In conclusion there are still few studies that focus on the molecular action of honey in keratinocytes and fail to report details on the honey used not allowing to achieve the same results.

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Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions

Cited by 11 publications

References 40 publications

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis

Molecular Mechanisms in the Process of Re-epithelization in Wound Healing and the Action of Honey in Keratinocytes

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