Modulating Expanded Polytetrafluoroethylene Vascular Graft Host Response via Citric Acid‐Based Biodegradable Elastomers

Yang, Jian; Motlagh, Delara; Allen, Josephine B.; Webb, Antonio R.; Kibbe, Melina R.; Aalami, Oliver; Kapadia, Muneera R.; Carroll, Timothy J.

doi:10.1002/adma.200600230

Cited by 90 publications

(77 citation statements)

References 34 publications

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“…The minimal generation and release of immunoreactive cytokines (TNF-a and IL-1b) from THP-1 cells upon incubation with POC, ePTFE, and PLGA supports the in vitro hemocompatibility of these materials and confirms in vivo biocompatibility data in pigs. 6 …”

Section: In Vitro Assessment Of Inflammatory Potentialmentioning

confidence: 99%

“…4 Specifically, poly(1,8-octanediol citrate) (POC), has recently been proposed for use as a biphasic scaffold for tissue engineering blood vessels and to improve the hemocompatibility of ePTFE vascular grafts. 5,6 However, the hemocompatibility of POC has not been fully investigated. Hemocompatibility characteristics will be especially important if an in vivo tissue engineering approach is pursued since the remodeling graft may have direct contact with flowing blood.…”

Section: Introductionmentioning

confidence: 99%

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Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering

Motlagh

Allen

Hoshi

et al. 2007

J Biomedical Materials Res

127

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One of the ongoing challenges in tissue engineering is the synthesis of a hemocompatible vascular graft. Specifically, the material used in the construct should have antithrombogenic properties and support the growth of vascular cells. Our laboratory has designed a novel biodegradable, elastomeric copolymer, poly(1,8-octanediol citrate) (POC), with mechanical and degradation properties suitable for vascular tissue engineering. The hemocompatibility of POC in vitro and its ability to support the attachment and differentiation of human aortic endothelial cell (HAEC) was assessed. The thrombogenicity and inflammatory potential of POC were assessed relative to poly(l-lactide-co-glycolide) and expanded poly(tetrafluoroethylene), as they have been used in FDA-approved devices for blood contact. Specifically, platelet aggregation and activation, protein adsorption, plasma clotting, and hemolysis were investigated. To assess the inflammatory potential of POC, the release of IL-1beta and TNF-alpha from THP-1 cells was measured. The cell compatibility of POC was assessed by confirming HAEC differentiation and attachment under flow conditions. POC exhibited decreased platelet adhesion and clotting relative to control materials. Hemolysis was negligible and protein adsorption was comparable to reference materials. IL-1beta and TNF-alpha release from THP-1 cells was comparable among all materials tested, suggesting minimal inflammatory potential. POC supported HAEC differentiation and attachment without any premodification of the surface. The results described herein are encouraging and suggest that POC is hemocompatible and an adequate candidate biomaterial for in vivo vascular tissue engineering.

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Section: In Vitro Assessment Of Inflammatory Potentialmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering

Motlagh

Allen

Hoshi

et al. 2007

J Biomedical Materials Res

127

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“…For example, POC supports the attachment, proliferation, and differentiation of a variety of vascular cells including human aortic smooth muscle and endothelial cells (EC), porcine endothelial cells isolated and differentiated in vitro from circulating progenitor cells in peripheral blood, and porcine smooth muscle and endothelial cells. 17,[33][34][35][36] In addition, when compared to ePTFE vascular grafts, POC was not thrombogenic and did not cause inflammation. 17,[33][34][35][36] However, before POC scaffolds can be used for in vitro cell culture and development studies it is important to understand the mechanical and degradation behavior of POC tubular scaffolds.…”

Section: Discussionmentioning

confidence: 94%

In Vitro Characterization of a Compliant Biodegradable Scaffold with a Novel Bioreactor System

et al. 2007

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The influence of scaffold compliance on blood vessel tissue engineering remains unclear and compliance mismatch issues are important to an in vivo tissue-engineering approach. We have designed and constructed a modular bioreactor system that is capable of imparting pulsatile fluid flow while simultaneously measuring vessel distension with fluid pressure changes in real time. The setup uses a pneumatic PID control system to generate variable fluid pressure profiles via LabVIEW and an LED micrometer to monitor vessel distension to an accuracy of +/-2 microm. The bioreactor was used to measure the compliance of elastomeric poly(1,8-octanediol citrate) (POC) scaffolds over physiologically relevant pressure ranges. The compliance of POC scaffolds could be adjusted by changing polymerization conditions resulting in scaffolds with compliance values that ranged from 3.8 +/- 0.2 to 15.6 +/- 4.6%/mmHg x 10(-2), depending on the distension pressures applied. Furthermore, scaffolds that were incubated in phosphate-buffered saline for 4 weeks exhibited a linear increase in compliance (2.6 +/- 0.9 to 7.7 +/- 1.2%/mmHg x 10(-2)) and were able to withstand normal physiological blood pressure without bursting. The ability to tailor scaffold compliance and easily measure vessel compliance in real time in vitro will improve our understanding of the role of scaffold compliance on vascular cell processes.

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“…The peaks at 3.388-3.764 ppm contributed the proton signal of -OCH 2 CH 2 -units from diol [26]. The multiple peaks around 7.005-7.527 ppm were attributed indole ring from indole-3-acetic acid [27], which has aromatic moieties present in polymeric product. This polymeric hydrogel was observed -NH peak at 10.724-10.970 ppm appeared from indole-3-acetic acid (Fig.…”

Section: Ft-irmentioning

confidence: 98%

Antioxidant Properties of Indole-3-Acetic Acid based Biopolymeric Hydrogels

Chitra¹,

Franklin²,

Sudarsan³

et al. 2017

Asian J. Chem.

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Modulating Expanded Polytetrafluoroethylene Vascular Graft Host Response via Citric Acid‐Based Biodegradable Elastomers

Cited by 90 publications

References 34 publications

Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering

Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering

In Vitro Characterization of a Compliant Biodegradable Scaffold with a Novel Bioreactor System

Antioxidant Properties of Indole-3-Acetic Acid based Biopolymeric Hydrogels

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