Modulating activity of fullerol C60(OH)22 on doxorubicin-induced cytotoxicity

Bogdanovic, Gordana; Kojić, Vesna; Đorđević, Aleksandar; Čanadanović-Brunet, Jasna; Vojinovic-Miloradov, Mirjana; Baltic, Vladimir

doi:10.1016/j.tiv.2004.02.010

Cited by 124 publications

(132 citation statements)

References 49 publications

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“…Although administration of GSH itself did not have an effect on embryonic susceptibility, uptake of GSH into cells is thought to be limited requiring ATP for transport (Wang et al, 1999). However, another study using embryonic zebrafish revealed that GSH co-exposure with tetrahydrofuran-C 60 (THF-C 60 ) increased survival when compared to THF-C 60 alone (Bogdanovic et al, 2004). In this case, it may be that solubilization of C 60 with THF changed its physicochemical properties such that GSH interacted directly with THF-C 60 in the exposure solution and reduced C 60 uptake.…”

Section: Discussionmentioning

confidence: 99%

“…Certain surface modifications (e.g. hydroxylation) have been shown to impart cytoprotective activity by eliminating reactive oxygen species (ROS) and antagonizing the effects of the oxidative stress-dependent cytotoxicity (Dugan et al, 2001;Bogdanovic et al, 2004;Isakovic et al, 2006). In fact, carboxylated-C 60 has been patented as a method to increase a metazoan's lifespan presumably through these same mechanisms (Dugan et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish

Usenko

Harper

Tanguay

2008

Toxicology and Applied Pharmacology

201

113

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Due to its unique physicochemical and optical properties, C 60 has raised interest in commercialization for a variety of products. While several reports have determined this nanomaterial to act as a powerful antioxidant, many other studies have demonstrated a strong oxidative potential through photoactivation. To directly address the oxidative potential of C 60 , the effects of light and chemical supplementation and depletion of glutathione (GSH) on C 60 -induced toxicity were evaluated. Embryonic zebrafish were used as a model organism to examine the potential of C 60 to elicit oxidative stress responses. Reduced light during C 60 exposure significantly decreased mortality and the incidence of fin malformations and pericardial edema at 200 and 300 ppb C 60 . Embryos co-exposed to the glutathione precursor, N-acetylcysteine (NAC), also showed reduced mortality and pericardial edema; however, fin malformations were not reduced. Conversely, co-exposure to the GSH synthesis inhibitors, butathionine sulfoximine (BSO) and diethyl maleate (DEM), increased the sensitivity of zebrafish to C 60 exposure. Co-exposure of C 60 or its hydroxylated derivative, C 60 (OH) 24 , with H 2 O 2 resulted in increased mortality along the concentration gradient of H 2 O 2 for both materials. Microarrays were used to examine the effects of C 60 on the global gene expression at two time points, 36 and 48 hours post fertilization (hpf). At both life stages there were alterations in the expression of several key stress response genes including glutathione-S-transferase, glutamate cysteine ligase, ferritin, α-tocopherol transport protein and heat shock protein 70. These results support the hypothesis that C 60 induces oxidative stress in this model system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish

Usenko

Harper

Tanguay

2008

Toxicology and Applied Pharmacology

201

113

View full text Add to dashboard Cite

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“…They have also exhibited antitumor and antiviral activity, including inhibition of HIV protease (Bogdanovic et al, 2004;Friedman et al, 1993;Nakamura and Isobe, 2003;Schinazi et al, 1993). Although the water-soluble fullerenes are not genotoxic (Zakharenko et al, 1997) they are retained in the body for long periods, raising concerns about chronic toxic effects (Yamago et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Phototoxicity and cytotoxicity of fullerol in human lens epithelial cells

Roberts

Wielgus

Boyes

et al. 2008

Toxicology and Applied Pharmacology

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The water-soluble, hydroxylated fullerene [fullerol, nano-C 60 (OH) [22][23][24][25][26] ] has several clinical applications including use as a drug carrier to bypass the blood ocular barriers. We have assessed fullerol's potential ocular toxicity by measuring its cytotoxicity and phototoxicity induced by UVA and visible light in vitro with human lens epithelial cells (HLE B-3). Accumulation of nano-C 60 (OH) [22][23][24][25][26] in the cells was confirmed spectrophotometrically at 405 nm and cell viability estimated using MTS and LDH assays. Fullerol was cytotoxic to HLE B-3 cells maintained in the dark at concentrations higher than 20 µM. Exposure to either UVA or visible light in the presence of >5 µM fullerol induced phototoxic damage. When cells were pretreated with non-toxic antioxidants: 20 µM lutein, 1 mM N-acetyl cysteine, or 1 mM L-ascorbic acid prior to irradiation, only the singlet oxygen quencher lutein significantly protected against fullerol photodamage. Apoptosis was observed in lens cells treated with fullerol whether or not the cells were irradiated, in the order UVA > visible light > dark. Dynamic light scattering (DLS) showed that in the presence of the endogenous lens protein α-crystallin, large aggregates of fullerol were reduced. In conclusion, fullerol is both cytotoxic and phototoxic to human lens epithelial cells. Although the acute toxicity of water soluble nano-C 60 (OH) 22-26 is low, these compounds are retained in the body for long periods, raising concern for their chronic toxic effect. Before fullerols are used to deliver drugs to the eye, they should be tested for photo-and cytotoxicity in vivo.

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“…The method has been extended and improved by several authors [7][8][9][10]. Doxorubicin (DOX, trade name Adriamycin) is an antitumor drug commonly used as single and in combination with other chemotherapeutic agents, for treatment of wide range of human malignancies [11,12].…”

Section: Introductionmentioning

confidence: 99%

Application of Artificial Neural Network for Modeling and Prediction of MTT Assay on Human Lung Epithelial Cancer Cell Lines

AA¹

2015

J Biosens Bioelectron

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Modulating activity of fullerol C60(OH)22 on doxorubicin-induced cytotoxicity

Cited by 124 publications

References 49 publications

Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish

Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish

Phototoxicity and cytotoxicity of fullerol in human lens epithelial cells

Application of Artificial Neural Network for Modeling and Prediction of MTT Assay on Human Lung Epithelial Cancer Cell Lines

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