Modulated Responses to Restraint Stress and Inflammation in Metallothionein-Null Mice

Suzuki, Shinya; Yamamoto, Masato; Sato, Masao

doi:10.1248/jhs.55.554

Cited by 2 publications

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References 29 publications

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“…MT is known as a cysteine-rich, low molecular weight and metal-binding protein (11) and has several interesting biological effects including detoxification of heavy metals, regulation of the homeostasis of essential metals, and antioxidant effects against reactive oxygen species (12)(13)(14)(15). We previously reported that food-induced dyslipidemia in mice could be accelerated by repeated fasting, but could be suppressed by repeated restraint, and that the DNA damage in cells of various organs was increased by the repeated stress, especially in MTnull mice (9).…”

Section: Originalmentioning

confidence: 99%

Preventive effects of metallothionein against DNA and lipid metabolic damages in dyslipidemic mice under repeated mild stress

et al. 2013

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The effects of repeated mild stress on DNA and lipid metabolic damages in multiple organs of dyslipidemic mice, and the preventive role of metallothionein (MT) were investigated. Female adult wild-type and MT-null mice fed high-fat diet (HFD) or standard diet (STD) were repeatedly subjected to fasting or restraint for three weeks. The liver, pancreas, spleen, bone marrow and serum samples were taken for evaluating DNA damage, MT, glutathione (GSH), corticosterone, carnitine and adiponectin. Body weights of restraint groups were reduced with the intensity of stress increased, even if the energy intakes were higher than those of STD group. Hepatic GSH levels were reduced in HFD control group and were further reduced in stress groups, especially in restraint groups, while the hepatic MT and serum corticosterone levels were increased in concert with the intensity of stress. Cellular DNA damages were generally increased by the restraint stress, especially in MT-null mice. Hepatic carnitine levels of MT-null mice were markedly lower than those of wild-type mice. The data suggest that MT plays a preventive role by acting as an antioxidant in corporation with GSH decreased by repeated stress and that MT may be an essential factor for inducing carnitine under the stress.

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