Modifying Fish Gelatin Electrospun Membranes for Biomedical Applications: Cross-Linking and Swelling Behavior

Padrão, Jorge; Silva, João Pedro; Rodrigues, L. R.; Dourado, Fernando; Lanceros‐Méndez, S.; Sencadas, Vítor

doi:10.1080/1539445x.2013.873466

Cited by 15 publications

(9 citation statements)

References 29 publications

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“…In recent years, research related to fish gelatin extraction and its application in biomedical engineering has increased due to its advantages over mammalian gelatin. First, economical production due to use of discarded byproducts of routine fish processing, unlike mammalian gelatin, the cost of which is influenced by that of raw materials [ 7 ]; and second, fewer personal or religious restrictions (e.g., vegetarianism, Judaism, Islam and Hinduism), who may be reluctant to use mammalian-origin biomaterials [ 7 , 24 – 27 ].…”

Section: Introductionmentioning

confidence: 99%

Cold Water Fish Gelatin Methacryloyl Hydrogel for Tissue Engineering Application

et al. 2016

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Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been used in various biomedical fields. Thus far, however, GelMA is mostly obtained from mammalian sources, which are associated with a risk of transmission of diseases, such as mad cow disease, as well as certain religious restrictions. In this study, we synthesized GelMA using fish-derived gelatin by a conventional GelMA synthesis method, and evaluated its physical properties and cell responses. The lower melting point of fish gelatin compared to porcine gelatin allowed larger-scale synthesis of GelMA and enabled hydrogel fabrication at room temperature. The properties (mechanical strength, water swelling degree and degradation rate) of fish GelMA differed from those of porcine GelMA, and could be tuned to suit diverse applications. Cells adhered, proliferated, and formed networks with surrounding cells on fish GelMA, and maintained high initial cell viability. These data suggest that fish GelMA could be utilized in a variety of biomedical fields as a substitute for mammalian-derived materials.

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Section: Introductionmentioning

confidence: 99%

Cold Water Fish Gelatin Methacryloyl Hydrogel for Tissue Engineering Application

et al. 2016

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“…GL has been also found to provide hemostasis and promote cell adhesion and proliferation during wound healing [24]. However, poor mechanical properties and low thermal stability, limits the biomedical application of this material which can be improved by cross-linking [25][26][27] and/or combining with other more stable polymers [7,21,28]. Research efforts for further improvement of GL nanofibrous matrices have been mainly targeting functionalization with pharmaceutical [29,30] and bioactive agents [30][31][32][33][34][35] as well as drug release modulation.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured scaffold with biomimetic and antibacterial properties for wound healing produced by ‘green electrospinning’

Tonda‐Turo

Ruini

Ceresa

et al. 2018

Colloids and Surfaces B: Biointerfaces

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The ECM-mimicking structure of GL-nanofibres was not affected by the presence of the antibacterial agents, which were homogeneously distributed within the mats as shown by SEM and EDS. The antibacterial properties of the developed matrices were confirmed using 4 strains (S. aureus, S. epidermidis, P. aeruginosa and E. coli) while the biocompatibility of the developed substrates and their ability to induce cell growth was assessed using Neonatal Normal Human Dermal Fibroblasts (NHDF-Neo).

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“…Statistical differences were found between uncross and crosslinked samples only for 0 and 1 % wt CPx. It was reported that the use of GA as a crosslinking agent for gelatin, did not changed significantly the sample fiber diameter [41,42]. Furthermore, the moisture content for the CPx-loaded formulations ( figure S2) was comparable to the drug free ones.…”

Section: Characterization Of Gel-cpx Particlesmentioning

confidence: 78%

Development and optimization of ciprofloxacin-loaded gelatin microparticles by single-step spray-drying technique

et al. 2018

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Polymeric particles are a versatile class of local or systemic drug delivery systems, used to improve drugs pharmacokinetics and pharmacodynamics along with patient compliance. Herein, we report a rapid, scalable, and optimized method to encapsulate ciprofloxacin (CP x ), a poor water soluble antimicrobial agent, in gelatin microparticles by single step processing via spray-drying of an aqueous solution. The developed particles show mainly a wrinkle morphology with a unimodal distribution, with mean diameters ranging between 2 and 4 μm, depending on the processing conditions. The encapsulation of 1, 2 and 5 wt% CPx narrows the size distribution (1-3 μm). In vitro release experiments showed that up to 80% of encapsulated drug is released during the first 6 h, and the release kinetics was best fitted with the Korsmeyer-Peppas model, explained by the superimposition of drug diffusion and polymer chain relaxation. The minimal inhibitory concentrations against S. aureus and E. coli, obtained from pure and encapsulated ciprofloxacin, demonstrated that the spray-drying process does not inhibit the drug's bioactivity or the process feasibility. Thus, spray-drying of protein-drug particle systems is an advantageous method to produce microparticles with potential to lung delivery systems. Smith, M. L. & Sencadas, V. (2018). Development and optimization of ciprofloxacin-loaded gelatin microparticles by single-step spray-drying technique. Powder Technology, 330 201-209. Abstract:Polymeric particles are a versatile class of local or systemic drug delivery systems, used to improve drugs pharmacokinetics and pharmacodynamics along with patient compliance. Herein, we report a rapid, scalable, and optimized method to encapsulate ciprofloxacin (CPx), a poor water soluble antimicrobial agent, in gelatin microparticles by single step processing via spray-drying of an aqueous solution. The developed particles show mainly a wrinkle morphology with a unimodal distribution, with mean diameters ranging between 2 and 4 , depending on the processing conditions. The encapsulation of 1, 2 and 5 wt% CPx narrows the size distribution (1-3 m). In vitro release experiments showed that up to 80% of encapsulated drug is released during the first 6 hours, and the release kinetics was best fitted with the Korsmeyer-Peppas model, explained by the superimposition of drug diffusion and polymer chain relaxation. The minimal inhibitory concentrations against S. aureus and E. coli, obtained from pure and encapsulated ciprofloxacin, demonstrated that the spraydrying process does not inhibit the drug`s bioactivity or the process feasibility. Thus, spray-drying of protein-drug particle systems is an advantageous method to produce microparticles with potential to lung delivery systems.

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Modifying Fish Gelatin Electrospun Membranes for Biomedical Applications: Cross-Linking and Swelling Behavior

Cited by 15 publications

References 29 publications

Cold Water Fish Gelatin Methacryloyl Hydrogel for Tissue Engineering Application

Cold Water Fish Gelatin Methacryloyl Hydrogel for Tissue Engineering Application

Nanostructured scaffold with biomimetic and antibacterial properties for wound healing produced by ‘green electrospinning’

Development and optimization of ciprofloxacin-loaded gelatin microparticles by single-step spray-drying technique

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