Cold Water Fish Gelatin Methacryloyl Hydrogel for Tissue Engineering Application

Abstract

Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been used in various biomedical fields. Thus far, however, GelMA is mostly obtained from mammalian sources, which are associated with a risk of transmission of diseases, such as mad cow disease, as well as certain religious restrictions. In this study, we synthesized GelMA using fish-derived gelatin by a conventional GelMA synthesis method, and evaluated its physical properties and cell responses. The lower melting point of fish gelatin compared to porcine gelatin allowed larger-scale synthesis of GelMA and enabled hydrogel fabrication at room temperature. The properties (mechanical strength, water swelling degree and degradation rate) of fish GelMA differed from those of porcine GelMA, and could be tuned to suit diverse applications. Cells adhered, proliferated, and formed networks with surrounding cells on fish GelMA, and maintained high initial cell viability. These data suggest that fish GelMA could be utilized in a variety of biomedical fields as a substitute for mammalian-derived materials.

The effect of alginate-gelatin encapsulation on the maturation of human myelomonocytic cell line U937

“…A greater attachment property and an increased proliferation rate were observed after the culture of bone marrow fibroblasts and mesenchymal stem cells on films coated with gelatin (Prasertsung et al, ). In light of higher viability values from our study and the previous experiments, it seems logical to state that cell morphological adaptation/rearrangement and growth dynamics are enhanced by an improved cell–material interaction and forming network after exposure to scaffolds enriched with gelatin (Haas, Banerji, & Culp, ; Yoon et al, ).…”

Electro‐Assisted Bioprinting of Low‐Concentration GelMA Microdroplets

“…The degradation profile of the microspheres is shown in Figure H. GelMA samples immersed in 2 U mL −1 collagenase II DPBS solution were almost completely degraded within 24 h, which indicated that the 5% (w/v) GelMA had the ability of responding to a biological environment and reflected its in vivo degradation profile to a certain extent (Figure S3, Supporting Information).…”

“…Different degree of methacrylation will lead to different crosslinking bonds. In our research, the GelMA reagent we chose in this paper is in a low degree of methacrylation . Thus, the elastic modulus of 5% (w/v) GelMA is low and it is more suitable for the cellular spreading.…”

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

“…As seen, FGSr was noted to degrade approximately 30% at a faster rate as compared to FG at week 1. As mentioned above, FG was noted to have fewer amino acids as compared to mammalian gelatin thus resulting in lower strength and higher swelling properties which leads to higher degradation [41]. In the FGSr scaffold, we speculate that the reason for the faster degradation rate may be in the light-cured FG matrix because the addition of SrCS powder affects the curing process and changes the ionic bonding between the FG matrix that accelerated the degradation rate.…”