The effect of alginate-gelatin encapsulation on the maturation of human myelomonocytic cell line U937

Nemati, Sorour; Sardroud, Hamed Alizadeh; Khoshfetrat, Ali Baradar; Khaksar, Majid; Ahmadi, Mahdi; Amini, Hassan; Saberianpour, Shirin; Delkhosh, Aref; Movassaghpour, Ali Akbar; Rahbarghazi‬, Reza

doi:10.1002/term.2765

Cited by 16 publications

(10 citation statements)

References 34 publications

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“…Cells were incubated for 30 min at different temperatures (4 and 37 • C) after been mixed within the bioink, consisting of A1.8 G3. For releasing the embedded cells, 0.1 M sodium citrate (pH = 7.4) was used as a chelating agent at 37 • C until the gel was dissolved, as previously described (Nemati et al, 2019). In the control group (C), cell viability was measured without the 30 min incubation step and mixture with bioink.…”

Section: Cell Viabilitymentioning

confidence: 99%

A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation

Ioannidis

Danalatos

Tsaniras

et al. 2020

Front. Bioeng. Biotechnol.

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Advances in 3D bioprinting have allowed the use of stem cells along with biomaterials and growth factors toward novel tissue engineering approaches. However, the cost of these systems along with their consumables is currently extremely high, limiting their applicability. To address this, we converted a 3D printer into an open source 3D bioprinter and produced a customized bioink based on accessible alginate/gelatin precursors, leading to a cost-effective solution. The bioprinter's resolution, including line width, spreading ratio and extrusion uniformity measurements, along with the rheological properties of the bioinks were analyzed, revealing high bioprinting accuracy within the printability window. Following the bioprinting process, cell survival and proliferation were validated on HeLa Kyoto and HEK293T cell lines. In addition, we isolated and 3D bioprinted postnatal neural stem cell progenitors derived from the mouse subventricular zone as well as mesenchymal stem cells derived from mouse bone marrow. Our results suggest that our low-cost 3D bioprinter can support cell proliferation and differentiation of two different types of primary stem cell populations, indicating that it can be used as a reliable tool for developing efficient research models for stem cell research and tissue engineering.

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Section: Cell Viabilitymentioning

confidence: 99%

A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation

Ioannidis

Danalatos

Tsaniras

et al. 2020

Front. Bioeng. Biotechnol.

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“…The U937 cells grown in suspension are characterized by a round shape, short microvilli, and a large beam-shaped nucleus (Pagliara et al, 2005;Tenuzzo et al, 2008). These monocytic cells bear the potential of differentiating into macrophages or dendritic cells; in response to a number of stimuli adopt the morphology and physiological characteristic of macrophages and thus represent a precursor of mononuclear phagocyte system (Kigerl et al, 2009;Italiani and Boraschi, 2014;Nemati et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Reactive Oxygen Species Imaging in U937 Cells

et al. 2020

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The U937 cell culture is a pro-monocytic, human histiocytic lymphoma cell line. These monocytes can differentiate into either macrophages or dendritic cells (antigenpresenting cells) depending on the initiators. The U937 cells activated in the presence of phorbol 12-myristate 13-acetate (PMA) change their morphology into macrophagelike cells creating pseudopodia and adhering generously. Macrophages are known to produce reactive oxygen species (ROS) mostly during phagocytosis of foreign particles, an important non-specific immune response. Recently, we have focused on the role of hydroxyl radical (HO •) and provide evidence on its importance for differentiation in U937 cells. Based on electron paramagnetic resonance (EPR) spectroscopy combined with confocal laser scanning microscopy (CLSM), formation of HO • was confirmed within the cells undergoing differentiation and/or apoptosis during the PMA treatment. This study aims to increase our knowledge of ROS metabolism in model cell lines used in human research.

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“…To overcome these limitations, protective microenvironments with proper biochemical and mechanical cues are essential to dictate specific cellular behavior and function [ 28 , 29 ]. In recent years, tissue engineering, a branch of interdisciplinary science, can help researchers and clinicians with accelerated tissue healing using de novo technological approaches consisting of specific cell populations, growth factors, and scaffolds [ 30 , 31 ]. In this article, recent data associated with the application of varied scaffold types for the regeneration of injured muscle tissue was investigated.…”

Section: Introductionmentioning

confidence: 99%

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

et al. 2023

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Muscular diseases and injuries are challenging issues in human medicine, resulting in physical disability. The advent of tissue engineering approaches has paved the way for the restoration and regeneration of injured muscle tissues along with available conventional therapies. Despite recent advances in the fabrication, synthesis, and application of hydrogels in terms of muscle tissue, there is a long way to find appropriate hydrogel types in patients with congenital and/or acquired musculoskeletal injuries. Regarding specific muscular tissue microenvironments, the applied hydrogels should provide a suitable platform for the activation of endogenous reparative mechanisms and concurrently deliver transplanting cells and therapeutics into the injured sites. Here, we aimed to highlight recent advances in muscle tissue engineering with a focus on recent strategies related to the regulation of vascularization and immune system response at the site of injury.

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The effect of alginate-gelatin encapsulation on the maturation of human myelomonocytic cell line U937

Cited by 16 publications

References 34 publications

A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation

A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation

Reactive Oxygen Species Imaging in U937 Cells

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

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