Modified Technique of Autologous Noncultured Epidermal Cell Transplantation for Repigmenting Vitiligo

Geel, Nanny van; Ongenae, Katia; Mil, Martine De; Naeyaert, Jean

doi:10.1097/00042728-200110000-00006

Cited by 9 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Noncultured melanocyte transplantation for the management of stable vitiligo is a recent addition to the therapeutic armamentarium for vitiligo and was first used successfully by Gauthier and Surleve‐Bazeille 6 . Since then, a number of independent researchers have used it with variable results 8–14 . Oral supplementation of Vitamin B12 has been shown to facilitate repigmentation in vitiligo by increasing pteridine metabolism, by increasing tyrosine concentration and downregulating plasma homocysteine levels 15 .…”

Section: Discussionmentioning

confidence: 99%

“…Van Geel and colleagues 10 obtained better results with a modified procedure in which hyaluronic acid was added to the noncultured melanocyte suspension to increase the viscosity of and prevent run‐off of the suspension from the dermabraded recipient site. They had failed to achieve successful repigmentation before employing modifications in the procedure, one of which was to add hyaluronic acid 9 …”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Autologous Noncultured Melanocyte Transplantation for Stable Vitiligo: Can Suspending Autologous Melanocytes in the Patients' Own Serum Improve Repigmentation and Patient Satisfaction?

Sahni¹,

Parsad²,

Kanwar³

et al. 2011

Dermatologic Surgery

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Autologous Noncultured Melanocyte Transplantation for Stable Vitiligo: Can Suspending Autologous Melanocytes in the Patients' Own Serum Improve Repigmentation and Patient Satisfaction?

Sahni¹,

Parsad²,

Kanwar³

et al. 2011

Dermatologic Surgery

View full text Add to dashboard Cite

show abstract

“…In this case the patient lived at considerable distance and natural sunshine was used by the patient. Previous work has shown that both autologous cultured and noncultured cell preparations are able to repigment vitiliginous areas 8,9 . The ease and speed of processing the donor site material and application to the recipient site of this new automated kit procedure would suggest its utility and warrant further investigation in larger studies.…”

Section: Discussionmentioning

confidence: 98%

An Automated Autologous Cell Transplantation Method for the Treatment of Hypopigmented Scarring

Goodman

2008

Dermatol Surg

View full text Add to dashboard Cite

The patient was a 35-year-old female who had a Jessner's augmented trichloroacetic acid chemical peel in July/August 1995. The procedure was followed by delayed wound healing with advent of keloidal and hypertrophic scar formation on the forehead and upper lip. Treatment at that time consisted of minocycline, intralesional corticosteroids, silicon sheeting, and the flashlamp-pumped dye laser. She also started on oral corticosteroids and colchicine and over the course of the next month the scarring stabilized.Despite these therapies, the patient still had significant residual scarring. This was a combination of atrophic and hypertrophic scars with broad areas of leukoderma on the forehead and upper lip and on both cheeks but more so on the right side.Further treatment by way of medication topically or orally was ceasing to make any change to her clinical condition. Thus, in 1996 she had the first of several laser-resurfacing procedures over the next decade. Each laser resurfacing decreased the hypertrophic scarring underneath her lip and her forehead but at no time did the hypopigmentation left over from these scars and in other areas diminish. During that time she also had silicon sheeting applied after the laser resurfacing had healed.In 2005 a commercially available single-use, standalone autologous cell harvesting device (ReCell, C3, Clinical Cell Culture, Cambridge, UK) was considered because of consistent areas of hypopigmentation, mainly on her upper lip and her forehead and her cheeks (Figure 1). MethodThe entire face including the recipient areas were treated with erbium laser resurfacing at 8 J/cm 2 per pulse with a 5-mm spot size. A double overlapping pass was performed to the entire face giving a total fluence in any given site of approximately 48 J. At an estimated 4 mm of ablation per joule of energy, this would approximate to ablate somewhere between 180 and 200 mm of tissue. This is sufficient to ablate the epidermis in all areas resurfaced.The right posterior neck donor site was prepared using 2% plain lidocaine. A one-piece razor bladetype instrument (DermaBlade, Personna Medical, Verona, VA) was used to shave a thin, split-thickness skin graft. This was placed in a well containing 10 mL of reconstituted trypsin. After about 20 minutes of trypsin digestion in the ReCell system, the skin graft was salvaged; the epidermis was stripped from the dermis with a scalpel blade and forceps and neutralized with 1.5 mL of the sodium lactate solution; and the resultant cell suspension was aspirated into a 5-mL syringe, gently expelled through a sieve, and then reaspirated into the 5-mL syringe. The suspension was then drizzled on the areas of the resurfaced skin only in areas of previous hypopigmentation. This included the forehead, the right

show abstract

“…It was concluded that it is a simple, safe, and effective surgical therapy. Van Geel et al (193) applied a melanocyte/keratinocyte suspension to vitiligo skin dermabraded using the CO 2 laser and found responses varying from 85% to 100%. Table III.…”

Section: Non-cultured Keratinocyte/melanocyte 'Grafting'mentioning

confidence: 99%

Vitiligo treatment options: An evolving scenario

Sehgal¹,

Srivastava²

2006

Journal of Dermatological Treatment

View full text Add to dashboard Cite

Sequential displays of several treatment options for vitiligo have been scanned from the literature, and are presented here. A few of the medical modalities have been resigned to history, whereas others have become customary in clinical practice. There has also been a recent surge of interest in the surgical treatment of this disease. Accordingly, this has been appraised and summarized. Special attention has been given to prevalent medical modalities so that they may be effectively utilized by those currently in practice.

show abstract

Modified Technique of Autologous Noncultured Epidermal Cell Transplantation for Repigmenting Vitiligo

Cited by 9 publications

References 9 publications

Autologous Noncultured Melanocyte Transplantation for Stable Vitiligo: Can Suspending Autologous Melanocytes in the Patients' Own Serum Improve Repigmentation and Patient Satisfaction?

Autologous Noncultured Melanocyte Transplantation for Stable Vitiligo: Can Suspending Autologous Melanocytes in the Patients' Own Serum Improve Repigmentation and Patient Satisfaction?

An Automated Autologous Cell Transplantation Method for the Treatment of Hypopigmented Scarring

Vitiligo treatment options: An evolving scenario

Contact Info

Product

Resources

About