Modified rapid urease test for <i>Helicobacter pylori</i> detection in relation to an immunohistochemical stain

Tokunaga, Yukihiko; Shirahase, Hiroyuki; Yamamoto, Etsuji; Inao, Ryou; Hamaguchi, Sugihiro; Kanaji, Kenji; Kitaoka, Akihiro; Yagi, Toshikazu; Tokuka, Atsuo; Ohsumi, Kiyoshi

doi:10.1046/j.1440-1746.2000.02213.x

Cited by 17 publications

(17 citation statements)

References 26 publications

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“…22 In the present study, there was a significant correlation between the severity of gastritis and the presence of H. pylori infection on histology. Similar results were seen by Tokunaga et al 23 In the present study, a total of six cases (10.0%) showed the presence of lymphoid follicles in addition to chronic inflammation. However, the presence of lymphoid follicles did not correlate significantly with the presence of H. pylori as only 3 out of these 6 patients were positive for H. pylori.…”

Section: Discussionsupporting

confidence: 92%

Correlation of serology with morphological changes in gastric biopsy of H. pylori infection

Rajan¹,

Ganguli

Pathak

et al. 2017

Int J Res Med Sci

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Background: Helicobacter pylori is involved in many gastrodeudonal complications and many diagnostic tests are available for its identification. The present study was done with the objective to evaluate the morphological changes induced by H. pylori in the gastric mucosa and to correlate them with the severity of the infection.Methods: This study was conducted in a tertiary care hospital from July 2013 to June 2014. 60 patients with symptoms of dyspepsia and requiring an upper gastrointestinal endoscopy were included in the study. Upper gastrointestinal endoscopy was performed on all patients. Hematoxylin and Eosin staining (H and E), modified Giemsa staining were performed on tissue sections and examined microscopically for gastritis and presence and absence of H. pylori.Results: Out of 60 patients, 33 were male and 27 were females. Serology by immunochromatography technique was positive in 41 patients. Serology was found to have a sensitivity and specificity of 90.90% and 59.25% respectively. H. pylori was positive in 28 cases on H and E. With a sensitivity and specificity of 84.84% and 100% respectively. H. pylori was positive in 33 cases on modified Giemsa with a sensitivity and specificity of 100%.Conclusions: Simultaneous morphologic and serological detection of H. pylori helps in its complete distribution and identification of its precancerous morphological nature.

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Section: Discussionsupporting

confidence: 92%

Correlation of serology with morphological changes in gastric biopsy of H. pylori infection

Rajan¹,

Ganguli

Pathak

et al. 2017

Int J Res Med Sci

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“…[39] found Giemsa to be positive in 78% patients and IHC in 89% and recommended Giemsa staining as a routine detection method. Similar results were obtained by Tokunaga et al[22]…”

Section: Discussionsupporting

confidence: 92%

“…The rapid urease test was found to be of less value in diagnosing H. pylori infection in our study with a sensitivity of 74.5% which is comparable to the findings of Tokunaga et al .,[22] Malik et al .,[23] and Ceken et al . [24] On the contrary, Calvet et al .…”

Section: Discussionsupporting

confidence: 89%

Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Shukla

Agarwal

Pujani

et al. 2012

Saudi J Gastroenterol

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Background/Aim:Helicobacter pylori is implicated in various gastroduodenal diseases and many tests are available for its detection. The present study attempted to document the morphological changes in the gastric mucosa induced by H. pylori colonization and correlate them with the severity of the infection. The study also compared various diagnostic tests and evaluated the different staining methods used for H. pylori detection, especially immunohistochemical identification.Patients and Methods:One hundred and two patients with dyspepsia were included. Enzyme-linked immunosorbent assay (ELISA) for H. pylori-specific immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) was used. Rapid urease test was performed on endoscopic biopsy and it was stained with hematoxylin and eosin (H and E), modified Giemsa, and immunohistochemical stains.Results:A significant correlation was found between the density of H. pylori and severity of gastritis. A significant correlation was observed between serology (especially when used in combination, IgG and IgA) and status of H. pylori. Immunohistochemical staining enhanced the diagnostic yield of H. pylori detection.Conclusions:Immunohistochemistry (IHC) should be used judiciously, whereas simple and economical tests like modified Giemsa should be used routinely for the detection of H. pylori. Combined ELISA (IgG and IgA) should be preferred over single ELISA. Simultaneous morphological and serological detection of H. pylori is preferable as H. pylori may not be detected on morphology alone due to its patchy distribution in the stomach.

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“…A limit to the urease test is the bacterial load necessary to obtain a sufficient sensitivity. A semiquantitative evaluation of the bacteria present by histology clearly showed that false-negative urease tests corresponded to the lowest histological scores for H. pylori (38,522,597). It seems that at least 10 5 bacteria are necessary for a valid result.…”

Section: Urease Testsmentioning

confidence: 99%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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SUMMARY The discovery of Helicobacter pylori in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases. It is now well accepted that the most common stomach disease, peptic ulcer disease, is an infectious disease, and all consensus conferences agree that the causative agent, H. pylori, must be treated with antibiotics. Furthermore, the concept emerged that this bacterium could be the trigger of various malignant diseases of the stomach, and it is now a model for chronic bacterial infections causing cancer. Most of the many different techniques involved in diagnosis of H. pylori infection are performed in clinical microbiology laboratories. The aim of this article is to review the current status of these methods and their application, highlighting the important progress which has been made in the past decade. Both invasive and noninvasive techniques will be reviewed.

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Modified rapid urease test for Helicobacter pylori detection in relation to an immunohistochemical stain

Cited by 17 publications

References 26 publications

Correlation of serology with morphological changes in gastric biopsy of H. pylori infection

Correlation of serology with morphological changes in gastric biopsy of H. pylori infection

Correlation of serology with morphological changes in gastric biopsy in Helicobacter pylori infection and evaluation of immunohistochemistry for H. pylori identification

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

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