<i>Helicobacter pylori</i>Detection and Antimicrobial Susceptibility Testing

Mégraud, Françis; Lehours, Philippe

doi:10.1128/cmr.00033-06

Cited by 618 publications

(676 citation statements)

References 562 publications

Supporting

Mentioning

618

Contrasting

Unclassified

Order By: Relevance

“…Among the various existing stains, one of the most recommended because of its simplicity and low cost is Giemsa staining (52,115,169,175) . Histological diagnosis and the urease test may show up to 95% sensitivity depending on the quality of material and expertise of the examiner (137,197) . Antisecretory drugs (H 2 receptor antagonists of histamine and proton pump inhibitors) and/or previous use of bismuth or antibiotics, by reducing the bacterial load, affect the accuracy of the urease test, so, whenever possible, an interval of two and 4 weeks respectively between suspending the drugs and the test is recommended (83,132) .…”

Section: Statementmentioning

confidence: 99%

See 1 more Smart Citation

3rd BRAZILIAN CONSENSUS ON Helicobacter pylori

Coelho

Maguinilk

Zaterka³

et al. 2013

Arq. Gastroenterol.

View full text Add to dashboard Cite

Significant progress has been obtained since the Second Brazilian Consensus Conference on Helicobacter pylori Infection held in 2004, in São Paulo, SP, Brazil, and justify a third meeting to establish updated guidelines on the current management of H. pylori infection. The Third Brazilian Consensus Conference on H pylori Infection was organized by the Brazilian Nucleus for the Study of Helicobacter, a Department of the Brazilian Federation of Gastroenterology and took place on April 12-15, 2011, in Bento Gonçalves, RS, Brazil. Thirty-one delegates coming from the five Brazilian regions and one international guest, including gastroenterologists, pathologists, epidemiologists, and pediatricians undertook the meeting. The participants were allocated in one of the five main topics of the meeting: H pylori, functional dyspepsia and diagnosis; H pylori and gastric cancer; H pylori and other associated disorders; H pylori treatment and retreatment; and, epidemiology of H pylori infection in Brazil. The results of each subgroup were submitted to a final consensus voting to all participants. Relevant data were presented, and the quality of evidence, strength of recommendation, and level of consensus were graded. Seventy per cent and more votes were considered as acceptance for the final statement. This article presents the main recommendations and conclusions to guide Brazilian doctors involved in the management of H pylori infection.

show abstract

Section: Statementmentioning

confidence: 99%

“…All serological tests must be locally validated (137,186) . In Brazil, validation studies have been performed in Campinas and Belo Horizonte (146,169) .…”

Section: Statementmentioning

confidence: 99%

3rd BRAZILIAN CONSENSUS ON Helicobacter pylori

Coelho

Maguinilk

Zaterka³

et al. 2013

Arq. Gastroenterol.

View full text Add to dashboard Cite

show abstract

“…5 Besides diagnosing active infection, culture also offers the possibility of performing resistance testing for a variety of antimicrobial agents. 1 Nevertheless, the sensitivity of biopsy-based tests and culture is significantly influenced by the density, viability and distribution of bacteria in the gastric mucosa. 6 In certain circumstances, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Advantages of non-invasive tests are mainly their non-invasiveness and their independency of bacterial colonization density and distribution variability of the pathogen in the gastric mucosa. 1 H. pylori can be detected noninvasively by serology, 2 stool antigen tests and by urea breath testing. 3,4 Gastroscopy, nevertheless, is often indicated in the management of H. pylori induced gastroduodenal disease.…”

Section: Introductionmentioning

confidence: 99%

Polymerase chain reaction versus culture in the diagnosis of Helicobacter pylori infection

Johannessen

Bergh

Jianu

et al. 2013

Gastroenterol Insights

View full text Add to dashboard Cite

In the management of Helicobacter pyloriinduced gastroduodenal disease, a pilot study at our hospital (St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway) revealed that culture often seemed to fail compared to the polymerase chain reaction (PCR). A more thorough evaluation was therefore undertaken. We included 201 patients referred to upper gastrointestinal endoscopy in the period [2002][2003][2004]. Serology, biopsy rapid urease test, culture and PCR were performed. Conventional PCR was performed using the ureC, vacA and cagA genes, and real-time PCR for ureC. A diagnostic standard was defined on the basis of all four tests, and all four tests were then compared to this standard. One hundred eleven patients were deemed H. pylori-positive by the defined diagnostic standard, and 90 were labeled negative. Compared to this standard, culture showed a sensitivity of 87.4%, which was significantly lower than PCR at 99.1% (P<0.001). Culture showed a perfect specificity of 100%, which was significantly better than PCR at 97.8%. ureC was the gene with the best sensitivity (94.6% in conventional PCR, 97.3% in real-time PCR). vacA sensitivity was 87.4%, which is significantly lower than ureC (P<0.001). cagA was present in 37.8% of our H. pylori-positive patients. By real-time PCR a significantly lower cycle threshold was observed in antral biopsies than in corpal biopsies, indicating a higher H. pylori DNA template concentration in antral biopsies. PCR-testing for H. pylori is faster and significantly more sensitive than culture. Culture on the other hand was significantly more specific than PCR in our hand.

show abstract

“…The key role of Helicobacter pylori (H pylori) in the pathogenesis of chronic gastritis, peptic ulcers, gastric cancer and mucosaassociated lymphoid tissue lymphoma is well recognized [1,2] . The treatment guidelines recommended for H pylori are a standard triple therapy consisting of clarithromycin and amoxicillin or metronidazole in combination with a proton pump inhibitor (PPI) [3] .…”

Section: Introductionmentioning

confidence: 99%

Transfer and distribution of amoxicillin in the rat gastric mucosa and gastric juice and the effects of rabeprazole

Zheng

Bao

et al. 2010

Acta Pharmacol Sin

View full text Add to dashboard Cite

Aim: To investigate the distribution of amoxicillin in the gastric juice and gastric mucosa of rats and to investigate the effects of proton pump inhibitor rabeprazole on amoxicillin concentrations in various compartments. Methods: One hundred and sixty anesthetized rats were divided into five groups, and given intravenously different doses of amoxicillin or amoxicillin and rabeprazole. The pH value and volume of gastric juice was aspirated were measured and separated gastric mucosa was homogenized. The concentrations of amoxicillin in the plasma, gastric juice and gastric mucosa were measured by high performance liquid chromatography (HPLC). Results: The maximum concentrations of amoxicillin in gastric juice and gastric mucosa were significantly lower than those in plasma (P<0.001). Concentrations in the glandular stomach mucosa were higher than those in the forestomach mucosa. Rabeprazole did not significantly change the pharmacokinetic parameters of amoxicillin in the plasma and did not alter gastric antibiotic clearance or the gastric transfer fraction of amoxicillin in gastric juice. However, rabeprazole did increase the amoxicillin concentration and pH value in gastric juice and reduced the volume of the gastric juice. Conclusion: Amoxicillin could penetrate the gastric mucosa and achieve therapeutic concentrations at the target site after transfer from the blood to the stomach. Rabeprazole increased the amoxicillin concentration in gastric juice by decreasing the gastric juice volume but did not affect its concentration in blood or gastric mucosa.

show abstract

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

Cited by 618 publications

References 562 publications

3rd BRAZILIAN CONSENSUS ON Helicobacter pylori

3rd BRAZILIAN CONSENSUS ON Helicobacter pylori

Polymerase chain reaction versus culture in the diagnosis of Helicobacter pylori infection

Transfer and distribution of amoxicillin in the rat gastric mucosa and gastric juice and the effects of rabeprazole

Contact Info

Product

Resources

About