Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe₃O₄@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity

Abstract

BackgroundNoble metal nanoparticles, due to their good physicochemical properties, have been exploited in biological applications. Among these metals, nanosilver has attracted great attention because of its optical properties and broad-spectrum antimicrobial activities with no drug tolerance.PurposeThe present study has attempted to conduct chemical synthesis of Fe3O4@PEG-Ag core/shell nanocomposites in aqueous solutions through co-precipitation of Fe3+ and Fe2+ ions, encapsulating the iron oxide core by poly (ethylene-glycol) (PEG) improve its hydrophilicity and biocompatibility, and immobilizing silver ions by application of NaBH4 as a reducing agent.Patients and methodsThe synthesized structures were characterized by Fourier-transform infrared (FT-IR), field emission scanning electron microscopy, energy-dispersive X-ray spectrum, wavelength-dispersive X-ray, vibrating sample magnetometer, inductively coupled plasma-mass spectrometry and transmission electron microscopy methods. Antimicrobial activity of the nanostructures against Staphylococcus aureus, Escherichia coli and Candida albicans was evaluated by broth microdilution based on the methods suggested by Clinical Laboratory Standard Institute. Furthermore, the nanocomposite was tested for possible anti-parasitic effects against Leishmania major promastigotes by MTT assay. Also, its impacts on bacterial cell morphology were defined using atomic force microscopy. Moreover, toxicity of the nanostructure related to animal cell line was determined based on MTT assay.ResultsIn general, the synthesized core/shell nanostructure can demonstrate noticeable activity against the evaluated representative microorganisms while its toxicity against animal cell line is not considerable.ConclusionThis nanostructure can be applied as a smart drug delivery system with the help of an external magnetic field or it can be used as a powerful antibiotic agent along with other antibiotics that can form a shell on its structure.

The Synthesis Methodology of PEGylated Fe3O4@Ag Nanoparticles Supported by Their Physicochemical Evaluation

“…The formation of the additional coating on the surface of the particles increase their functionality via the possibility of the attachment of active substances to such modified surfaces and, importantly, reduces the agglomeration of these particles which spontaneously tend to form clusters (i.e., they behave like small magnets). The correct course of PEGylation process may be also confirmed, comparing the results of the investigations performed with data in the literature, where the same FT-IR spectra of PEG coated particles have been presented [ 53 ]. Thus, the synthesis methodology presented here enables us to conclude that the chosen research direction, as well as the results obtained, are consistent with the scientific trend concerning the formation of core–shell nanoparticles presented by other researchers.…”

Application of luteinizing hormone-releasing hormone–ferrosoferric oxide nanoparticles in targeted imaging of breast tumors

“…After the addition of 450 mL of water, the iron concentration of the samples was determined by atomic emission spectrometry as previously described. 14 The measured iron concentrations were normalized to the cell number of the samples.…”

Nano-Based Drug Delivery or Targeting to Eradicate Bacteria for Infection Mitigation: A Review of Recent Advances

“…The nanocomposite was more effective in eradicating Gram-negative bacteria with an inhibition zone of 34-39 mm than Gram-positive bacteria (30-32 mm). Zomorodian et al (2018) attempted to encapsulate magnetic Fe 3 O 4 by PEG for improving hydrophilicity and biocompatibility. Ag as the antimicrobial agent was coated onto the nanoparticulate shell.…”