Modified light spectral conditions prior to cryopreservation alter growth characteristics and cryopreservation success of potato (Solanum tuberosum L.) shoot tips in vitro

Edesi, Jaanika; Pirttilä, Anna Maria; Häggman, Hely

doi:10.1007/s11240-016-1119-x

Cited by 18 publications

(10 citation statements)

References 37 publications

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“…Yoon et al [ 45 ] reported that several potato cultivars grown under high light intensity (140 nmol m −2 s −1 ) prior to cryopreservation resulted in significantly higher post -cryopreservation recovery. Edesi et al [ 119 ] showed that blue light promoted growth potential, photomorphogenesis, and subsequent survival after cryopreservation of potato clones, while Mølmann et al [ 120 ] showed that the red light produced strong inhibition of sprout elongation even at low irradiances 10–100 nmol m −2 s −1 in the same species. Nonetheless, the effect of modified light conditions on cryopreservation efficiency is still not well studied.…”

Section: Cryopreservation Methodsmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

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Section: Cryopreservation Methodsmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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“…Light is a crucial environmental factor that influences the in vitro growth and development of potato plantlets [3] , because they are strongly affected by the light spectrum [4] . Light spectral quality prior to cryopreservation can influence potato growth characteristics in vitro, and the success of shoot tip cryopreservation and subsequent survival [5] . It was reported that potato plantlets grown in vitro under red light were weak and slim with small leaves while those under blue light were short and sturdy with well-developed leaves [6] .…”

Section: Introductionmentioning

confidence: 99%

Effects of red and blue LEDs on in vitro growth and microtuberization of potato single-node cuttings

2018

Front. Agr. Sci. Eng.

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The objectives of this study were to investigate the effects of red and blue LEDs on in vitro growth and microtuberization of potato (Solanum tuberosum) singlenode cuttings. Explants were incubated under 6 light treatments: 100% red LEDs (R), 75% red LEDs + 25% blue LEDs (3RB), 50% red LEDs + 50% blue LEDs (RB), 25% red LEDs + 75% blue LEDs (R3B), 100% blue LEDs (B) and white LEDs (W). Most of the growth and physiological parameters were significantly higher in 3RB than W. Enhancement of leaf area and chlorophyll concentrations were obtained in B. Leaf stomata were elliptical with the lowest density in 3RB. However, those in W were round in shape, and those with the smallest size and the highest density were observed in R. Most of the characteristics of microtuberization were also improved in 3RB. The combined spectra of red and blue LEDs increased the number of large microtubers. The fresh weight of individual microtubers in R and W were increased, but not their number. These results suggest that, of the treatments assessed, 3RB is optimal for the in vitro growth of potato plantlets and the combination of red and blue LEDs is beneficial for microtuberization.

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“…The recovery of cryopreserved tissue is a critical part of the cryopreservation process and it depends on, among other factors, the subsequent culture conditions (Walters et al, 2008;Edesi et al, 2017). Media enriched with plant growth regulators, such as kinetin and indoleacetic acid, were used in the growth and recovery phase for Araucaria hunsteinii embryos and a high rate of recovery was obtained (Pritchard & Prendergast, 1986).…”

Section: Discussionmentioning

confidence: 99%

Viability of embryonic axes of Araucaria angustifolia after freezing using two cryopreservation methods

Frizzo

Quoirin

2018

Pesq. flor. bras.

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Araucaria angustifolia (Bertol.) O. Kuntze é uma das espécies nativas do Brasil com maior importância na Região Sul. No entanto, suas sementes são recalcitrantes, constituindo um obstáculo para a sua conservação em longo prazo, sendo a criopreservação uma alternativa viável para o armazenamento de seu germoplasma. Os eixos embrionários (EE) excisados das sementes de araucária foram encapsulados, desidratados e submetidos a dois métodos de criopreservação: o resfriamento rápido, congelando em nitrogênio líquido (NL) durante 2 h, e o pré-resfriamento a - 40 °C, seguido do congelamento em nitrogênio líquido durante 2 h. Posteriormente, os EE foram descongelados rapidamente e avaliados quanto à integridade do DNA pelo teste bioquímico do tetrazólio, germinação in vitro e ocorrência de oxidação. Tanto o DNA dos EE que não foram criopreservados quanto o daqueles que foram criopreservados mantiveram sua integridade. Os resultados do teste de tetrazólio indicaram que a maioria dos EE desidratados pelo congelamento rápido permaneceu viável. Após 15 dias de cultivo in vitro, os EE não germinaram e apresentaram sinais de oxidação. Os métodos de encapsulamento-desidratação seguidos de congelamento rápido são promissores para a criopreservação de EE de araucária, como demonstrado pelo teste de tetrazólio e da manutenção da integridade total do DNA.

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Modified light spectral conditions prior to cryopreservation alter growth characteristics and cryopreservation success of potato (Solanum tuberosum L.) shoot tips in vitro

Cited by 18 publications

References 37 publications

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Effects of red and blue LEDs on in vitro growth and microtuberization of potato single-node cuttings

Viability of embryonic axes of Araucaria angustifolia after freezing using two cryopreservation methods

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