Modified Incompatibility of Buckwheat Following Irradiation

Sharma, Kamal Dev; Boyes, J. W.

doi:10.1139/b61-108

Cited by 43 publications

(23 citation statements)

References 6 publications

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“…This trait is mainly controlled by a single genomic region, the so called self-incompatibility complex or S gene, where short-styled plant is heterozygote (Ss) and long-styled plant is recessive homozygote (ss) (Sharma and Boyes, 1961). Since limited genetic heterogeniety among BACs is desirable while constructing contigs from overlapping clones, a breeding experiment was conducted as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Construction of a BAC library for buckwheat genome research -An application to positional cloning of agriculturally valuable traits-

et al. 2008

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We have constructed a BAC library for common buckwheat Fagopyrum esculentum Moench. The library includes 142,005 clones with an average insert size of ~76 kb, equivalent to approximately a 7~8-fold coverage of the genome. Polymerase chain reaction based screening of the library with AGAMOUS and FLORICAULA/ LEAFY primers, has identified 7 and 9 BACs, respectively, which are consistent with the genome coverage. This library represents the first large insert genomic library for F. esculentum and it can be served as a genetic resource facilitating agricultural, pharmacological, physiological, and evolutionary studies of the species. To demonstrate the utilization of the library for characterizing agriculturally valuable traits, we developed a sequence tagged site marker tightly linked to the dwarf E locus as well as to the self-incompatibility complex locus and screened the library to initiate positional cloning of the causative genes.

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Section: Methodsmentioning

confidence: 99%

Construction of a BAC library for buckwheat genome research -An application to positional cloning of agriculturally valuable traits-

et al. 2008

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“…Common buckwheat (Fagopyrum esculentum Moench) is a typical species showing outcrossing and self-incompatiblity belonging to the family Polygonaceae Sharma and Boyes, 1961). Common buckwheat has been widely distributed and a cultivated crop of considerable importance in many countries around the world, in Asia, America and Europe, although the cultivation of this crop has not increased in recent years (Alekseeva, 1986;Kump and Javornik, 1996).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Diversity and Population Structure of Buckwheat (Fagopyrum esculentum Moench) Landraces of Korea Using SSR Markers

Song

Lee

Yoon

et al. 2011

Korean Journal of Plant Resources

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-Buckwheat (Fagopyrum esculentum Moench), one of the minor crops grown in Korea belonging to the Polygonaceae family, is an annual crop widely cultivated in Asia, Europe, and America and has a character of outcrossing and self-incompatibility. The objective of this study was to analyze the genetic variability, phylogenetic relationships and population structure of buckwheat landraces of Korea using SSR markers. Ten microsatellite markers have been detected from a total of 79 alleles among the 179 buckwheat accessions were collected from Korea. The number of allele per marker locus (NA) ranged from 2 (GB-FE-001, GB-FE-043 and GB-FE-055) to 31 (GB-FE-035) with an average of 7.9 alleles. GB-FE-035 was the most polymorphic with the highest PIC value 0.93. Major allele frequencies (MAF) for the 10 polymorphic loci varied from 0.12 to 0.97 with a mean allele frequency of 0.57. The expected heterozygosity (HE) values ranged from 0.05 to 0.94 with an average of 0.53. The observed heterozygosity (HO) ranged from 0.06 to 0.92 with an average of 0.42. The overall polymorphic information contents (PIC) values ranged from 0.05 to 0.93 with an average of 0.48. The landrace accessions of buckwheat used in the present study were not distinctly grouped according to geographic distribution. The study concludes that the results revealed genetic differentiation was low according to the geographic region because of outcrossing and self-incompatibility. We reported that our analyses on the genetic diversity of common buckwheat cultivars of Korea were performed by using of microsatellite markers.

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“…The genus Fagopyrum is thought to comprise at most 19 species of different breeding systems such as heterostylous self-incompatible, homostylous self-compatible, and heterostylous self-compatible (Sharma and Boys, 1961;Adachi et al, 1982;Ohnishi, 1993Ohnishi, , 1995Ohnishi, , 1998Ohsako and Ohnishi, 1998). Common buckwheat ( F. esculentum ) is a cultivated heterostylous self-incompatible species that comprises two mating types, pin and thrum.…”

Section: Introductionmentioning

confidence: 99%

Topological incongruence between nuclear and chloroplast DNA trees suggesting hybridization in the urophyllum group of the genus Fagopyrum (Polygonaceae)

2003

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We performed phylogenetic analyses of Fagopyrum species in the urophyllum group based on nucleotide sequences of two nuclear genes, FLORICAULA/LEAFY ( FLO/LFY ) and AGAMOUS ( AG ), and three segments of chloroplast DNA (cpDNA), rbc L-acc D, trn K intron, and trn C-rpo B spacer. The FLO/LFY and AG sequences turned out to be phylogenetically more informative at the intrageneric level than the cpDNA sequences. Congruence among these gene trees, inferred by a maximum-likelihood (ML) method, demonstrated that topologies were partially incongruent between the nuclear and chloroplast DNA phylogenies. The nuclear DNA sequence data supported a monophyletic relation of F. statice , F. gilesii , and F. jinshaense , whereas the former two species formed another monophyletic relation with the F. capillatum -F. gracilipes -F. gracilipedoides -F. rubifolium clade excluding F. jinshaense in the synthetic cpDNA phylogeny. In addition, two divergent sequences of FLO/LFY were found in F. rubifolium (tetraploid). One of these was sister to F. gracilipedoides and another was sister to F. statice , and a monophyletic relation of these two genes was rejected by a bootstrap analysis. These results suggest that hybridization may have occurred during diversification of Fagopyrum species in the urophyllum group, and that F. rubifolium is possibly allotetraploid species.

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Modified Incompatibility of Buckwheat Following Irradiation

Cited by 43 publications

References 6 publications

Construction of a BAC library for buckwheat genome research -An application to positional cloning of agriculturally valuable traits-

Construction of a BAC library for buckwheat genome research -An application to positional cloning of agriculturally valuable traits-

Analysis of Genetic Diversity and Population Structure of Buckwheat (Fagopyrum esculentum Moench) Landraces of Korea Using SSR Markers

Topological incongruence between nuclear and chloroplast DNA trees suggesting hybridization in the urophyllum group of the genus Fagopyrum (Polygonaceae)

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