Modified in vivo behavior of liposomes containing synthetic glycolipids

Wu, Margaret; Robbins, James C.; Bugianesi, Rossana; Ponpipom, Mitree M.; Shen, T. Y.

doi:10.1016/0304-4165(81)90342-1

Cited by 101 publications

(24 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the dynamic flow of the plasmid DNA will be different when a large volume and high injection rate are used. In mice with body weight of 18-20 g, an injection volume of 1.6 ml per mouse is almost equivalent to the total blood volume of the animal (blood volume is estimated to be 7.3% of the body weight for mice 25 ) and the injected DNA solution is likely to accumulate in the inferior vena cava when the injection rate exceeds the cardiac output. As a result, a high hydrostatic pressure develops in the inferior vena cava.…”

Section: Discussionmentioning

confidence: 99%

Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA

1999

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA

1999

View full text Add to dashboard Cite

“…Lipid suspenmouse equals 7.3% of total body weight. 23 The blood consion was homogenized for 2-3 min using a Tissue Tearor tamination in each organ was corrected using a pre-(Biospec Products, Racine, WI, USA) at the maximal viously described method. 24 Results were expressed as a speed (20 000 r.p.m.).…”

Section: Tissue Distribution Preparation Of Dna-lipid Complexesmentioning

confidence: 99%

Factors controlling the efficiency of cationic lipid-mediated transfection in vivo via intravenous administration

et al. 1997

View full text Add to dashboard Cite

The factors controlling the transfection efficiency of cationic transfection efficiency was dependent on both DOTMA to lipid carrier systems following intravenous administration DNA, and DOTMA to Tween 80 ratios. Among the organs are poorly understood. Using N-[1-(2,3-dioleoyloxy)propyl]-examined, the lung appeared to be more transfectable than N,N,N-trimethylammonium chloride (DOTMA) combined other organs. A better transfection activity was obtained with Tween 80 as a carrier system and cDNA of luciferase with higher DOTMA to DNA and DOTMA to Tween 80 or ␤-galactosidase gene as a reporter, we investigated the ratios. Time-response curve shows that gene expression importance of DOTMA to DNA ratio and the ratio of was transient with a maximal level between 10 and 24 h DOTMA to Tween 80 in the lipid formulation in determining after injection. Results from tissue distribution studies with the site and level of transgene expression following intra-125 I-labeled plasmid DNA and Southern analysis suggest venous administration. The data show that all of the that the transient expression is the result of the loss of internal organs, including lung, liver, spleen, heart and kidtransgene from the transfected cells. These results suggest neys, expressed the transgene upon systemic administhat cationic lipid-based delivery systems can be efficient tration into animals with 25 g of plasmid DNA when comfor gene delivery if the composition of the DNA-lipid complexed with DOTMA-Tween 80 lipid formulation. The plexes is properly controlled.

show abstract

“…The radioactivity in the blood was estimated with the assumption that the total blood in mouse equals to 7.3% of total body weight. 33 The blood contamination in each organ was corrected using correction factors established from tissue distribution studies of 51 Cr-labeled red blood cells in mouse. 34 …”

Section: Transfection Of Mouse Lung In Vitromentioning

confidence: 99%

Enhanced gene expression in mouse lung by prolonging the retention time of intravenously injected plasmid DNA

Song

Liu

1998

Gene Ther

View full text Add to dashboard Cite

The effect of retention time of plasmid DNA in mouse lung cholesterol-based cationic liposomes to retain the plasmid on the level of transgene expression after intravenous DNA in the lung. The level and patterns of gene expression administration was examined. Using CMV driven obtained appeared similar to those seen in animals transexpression system with luciferase gene as a reporter and fected by DNA-liposome complexes. These results sugpreinjection of free cationic liposomes into the animal as gest that prolonging the exposure time of DNA to the target means of manipulating the retention time of plasmid DNA, cells in vivo may be an important strategy in achieving a we demonstrated that naked plasmid DNA is effective in high level of gene expression. Our data also introduce transfecting cells in the lung by intravenous administration.a possibility that the function of cationic liposomes in An increase in DNA retention time in the lung results in a lipoplex-mediated transfection in vivo is to extend the higher level of gene expression. Liposomes composed of interaction time of DNA with the cells. cationic lipids with alkyl chains exhibited better activity than

show abstract

Modified in vivo behavior of liposomes containing synthetic glycolipids

Cited by 101 publications

References 18 publications

Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA

Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA

Factors controlling the efficiency of cationic lipid-mediated transfection in vivo via intravenous administration

Enhanced gene expression in mouse lung by prolonging the retention time of intravenously injected plasmid DNA

Contact Info

Product

Resources

About