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The effect of retention time of plasmid DNA in mouse lung cholesterol-based cationic liposomes to retain the plasmid on the level of transgene expression after intravenous DNA in the lung. The level and patterns of gene expression administration was examined. Using CMV driven obtained appeared similar to those seen in animals transexpression system with luciferase gene as a reporter and fected by DNA-liposome complexes. These results sugpreinjection of free cationic liposomes into the animal as gest that prolonging the exposure time of DNA to the target means of manipulating the retention time of plasmid DNA, cells in vivo may be an important strategy in achieving a we demonstrated that naked plasmid DNA is effective in high level of gene expression. Our data also introduce transfecting cells in the lung by intravenous administration.a possibility that the function of cationic liposomes in An increase in DNA retention time in the lung results in a lipoplex-mediated transfection in vivo is to extend the higher level of gene expression. Liposomes composed of interaction time of DNA with the cells. cationic lipids with alkyl chains exhibited better activity than

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Background: Adipose-derived stem cell therapy has shown much promise in realm of wound healing, but research into its use speci cally for burn wounds is limited. In this study, we hypothesize that adipose derived stem cells will improve the quality and rate of burn wound healing in a mouse model. Methods:Twenty athymic nude mice were subjected to nonfatal full thickness burns, using a 1cm diameter brass stamp heated to 70°C and applied to the paraspinal region for 10 seconds. On post-burn day 1, animals were randomized to receive a one time sub-eschar injection of either ADSCs suspended in sterile PBS (6.8x10 6 cells per injection; treatment group) or sterile PBS only (control group). Wounds were photographed three times weekly to measure the rate of wound healing. Mice from each group were sacri ced on post burn days 4,7,14,21. Wound tissues were evaluated histologically with H +E, Mason Trichrome, Picroserius, and CD31 staining to evaluate wound healing. Molecular studies measured markers of in ammation, adipogenesis and collagen formation.