Modified Ellman procedure for assay of cholinesterases in crude enzymatic preparations

Gorun, Victoria; Proinov, Ioan; Băltescu, Valeria; Balaban, Gabriela; Bârzu, Octavian

doi:10.1016/0003-2697(78)90350-0

Cited by 209 publications

(89 citation statements)

References 3 publications

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“…The procedure used for the determination of AchE activity in the heart was a modification of the method of Ellman et al (1961) as described by Gorun et al (1978). The principle of the method is the measurement of the thiocholine produced as acetylthiocholine is hydrolyzed.…”

Section: Determination Of Enzyme Activitiesmentioning

confidence: 99%

The effect of bisphenol A on some oxidative stress parameters and acetylcholinesterase activity in the heart of male albino rats

Khadrawy²,

2013

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Bisphenol A (BPA) is an endocrine disrupting chemical used on a wide range in industry. Several studies reported that BPA may cause cardiovascular disorders in humans and animals. The present study aims to investigate the effect of BPA on the heart of adult male rats. The rats received a daily oral administration of BPA (25 mg/kg for 6 weeks and 10 mg/kg for 6 and 10 weeks). It was found that BPA at the two studied doses induced a significant increase in malondialdehyde, and a significant decrease in catalase after 6 weeks. Moreover, a significant decrease in reduced glutathione and acetylcholinesterase (AchE) activity was observed after treatment with the two doses of BPA throughout the studied time intervals. The two doses (25 and 10 mg/kg) resulted in a significant decrease in nitric oxide (NO) levels after 6 and 10 weeks, respectively. A significant increase in body weight gain occurred in all animals after BPA treatment. These results suggest that BPA has cardiotoxic effects which are mediated by the oxidative stress resulting from the overproduction of free radicals, the deficiency of NO and the inhibition of AchE leading to cholinergic activation. The obesity promoting effect of BPA may also participate in the observed cardiovascular disturbances.

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Section: Determination Of Enzyme Activitiesmentioning

confidence: 99%

The effect of bisphenol A on some oxidative stress parameters and acetylcholinesterase activity in the heart of male albino rats

Khadrawy²,

2013

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“…The plate could not be dried absolutely otherwise the enzyme could be inactivated. However, ethanol should be removed because it might inhibit acetylcholinesterase activity [5]. The TLC plate was put in a closed vessel containing a little water for humidity whereas plate should not contact with water directly.…”

Section: Modified Tlc Bioautographic Assaymentioning

confidence: 99%

“…Ellman's method is a classical method in detecting acetylcholinesterase inhibitors [4,5]. However, the results will be uncertain when testing colored samples or hydrophobic samples.…”

Section: Introductionmentioning

confidence: 99%

Modified TLC bioautographic method for screening acetylcholinesterase inhibitors from plant extracts

Yang

Zhang

Duan

et al. 2009

J of Separation Science

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The present work describes modifications to an existing TLC bioautographic method for detecting acetylcholinesterase inhibitors from plant extracts. The basic principle of the method is that the enzyme converts 1-naphthyl acetate into naphthol which reacts with Fast Blue B salt to make a purple-colored background on the TLC plates. Inhibitors of acetylcholinesterases produced white spots on the background. Our modifications involve changes in the concentration of the enzyme, the reagents, and the time of the reaction. With these changes, the consumption of the enzyme was reduced by 85% and the detection limits were decreased remarkably.

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“…The blood in non-heparinized tubes was centrifuged at 3000 rpm for 20 min for other biochemical parameters. Cholinesterase activity was determined according to Ellman Method (28) as modified by Gorun et al (29) . The liver parameters [Alanine aminotrasferase (ALT), Aspartate aminotrasferase (AST), alkaline phosphates (ALP), total protein and albumin], kidney parameters (urea and creatinine), lipid profile (cholesterol, triglycerides) and antioxidant parameters [Catalase (CAT) and Glutathione-S-Transfrase (GST)] were determined by using local kits (from Biodiagonostic Company in Egypt).…”

Section: Toxicological Studiesmentioning

confidence: 99%

Fate of 14C-ethyl Profenofos in Soybean Seeds and Oils, Residue Removal, Bioavailability, Toxicity and Protective Action of Cinnamon Extract towards Experimental Animals

2011

Egypt. J. Chem.

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Modified Ellman procedure for assay of cholinesterases in crude enzymatic preparations

Cited by 209 publications

References 3 publications

The effect of bisphenol A on some oxidative stress parameters and acetylcholinesterase activity in the heart of male albino rats

The effect of bisphenol A on some oxidative stress parameters and acetylcholinesterase activity in the heart of male albino rats

Modified TLC bioautographic method for screening acetylcholinesterase inhibitors from plant extracts

Fate of 14C-ethyl Profenofos in Soybean Seeds and Oils, Residue Removal, Bioavailability, Toxicity and Protective Action of Cinnamon Extract towards Experimental Animals

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