Modified CTAB Method for High Quality Genomic DNA Extraction from Medicinal Plants

Attitalla, Idress Hamad

doi:10.3923/pjbs.2011.998.999

Cited by 48 publications

(20 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Powder was transferred into a 50 mL tube containing 10 mL sterile water and centrifuged at 200×g for 20 min, 500×g for 20 min and 16, 500×g for 15 min at 4°C. Total genomic DNA was extracted from the precipitate using the CTAB method with slight modifications [39].…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Temporal and spatial pattern of endophytic fungi diversity of Camellia sinensis (cv. Shu Cha Zao)

Cui

et al. 2020

BMC Microbiol

View full text Add to dashboard Cite

Background: The experimental materials were a 60-year-old tea tree (Camellia sinensis cv. Shu Cha Zao; SCZ) (the mother plant) and 1-year-old and 20-year-old plants of SCZ that originated as mother plant cuttings. The aim of this study was to use high-throughput sequencing to study the spatial and dynamic distribution of endophytic fungi in different leaf niches (upper leaves, middle leaves, lower leaves) and rhizosphere soil on tea plants of different ages in the same garden. Results: Ascomycota (83.77%), Basidiomycota (11.71%), and Zygomycota (3.45%) were the dominant fungal phyla in all samples. Cladosporium (12.73%), Zymoseptoria (9.18%), and Strelitziana (13.11%) were the dominant genera in the leaf. Alpha diversity analysis revealed that endophytic communities in leaves differed from those in rhizosphere soil and different leaf niches had similar fungal diversity. Shannon's indices and NMDS analysis indicated significant differences in fungal diversity and composition among the SCZ trees of different ages (p ≤ 0.01). The abundance of Cladosporium and Zymoseptoria decreased with increasing SCZ age, whereas the abundance of Strelitziana increased. Conclusions: The results illustrate variation in endophytic fungi among different niches on tea plants of different ages. The distribution of endophytic fungi in leaves of C. sinensis shows spatiotemporal variation.

show abstract

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Temporal and spatial pattern of endophytic fungi diversity of Camellia sinensis (cv. Shu Cha Zao)

Cui

et al. 2020

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…Effective CTAB-based protocols have been described to obtain DNA from soybean, https://doi.org/10.17221/24/2021-CJFS maize (Demeke and Jenkins 2010), various cereal grains, oilseed rape (Park et al 2010;Li et al 2013), many of the fruits used to produce infusions (e.g. apple, pear, apricot and raspberry) (Lodhi et al 1994), medicinal and aromatic plants and even from herbarium samples (Cota--Sánchez et al 2006;Attitalla 2011). As an alternative, a number of commercial DNA isolation kits have been developed which rely on either anion exchange chromatography or silica-gel membrane (Gaikwad 2002).…”

mentioning

confidence: 99%

Comparison of methods to extract PCR-amplifiable DNA from fruit, herbal and black teas

Čermáková¹,

Zdeňková²,

Demnerová³

et al. 2021

Czech J. Food Sci.

View full text Add to dashboard Cite

The success of polymerase chain reaction (PCR) assay depends on template deoxyribonucleic acid (DNA) being sufficient with respect to both quantity and quality. Some biological materials contain compounds which inhibit the functioning of DNA polymerase and thus need to be removed as part of the DNA extraction procedure. The aim of the present experiments was to optimise the process of DNA isolation from various types of black, fruit and herbal teas. A comparison was made between two cetyltrimethylammonium bromide (CTAB)-based protocols and two commercially available DNA purification kits. The yield and integrity of the extracted DNA were monitored both spectrophotometrically and using agarose gel electrophoresis. The presence/absence of inhibitors in the DNA preparations was checked by running quantitative real-time PCRs. The optimal protocol was deemed to be the CTAB method described in ISO 21571:2005, so this method is recommended for the routine sample analysis of tea products.

show abstract

“…To develop an efficient and rapid DNA extraction protocol, four different methods for DNA extraction from EBN were evaluated. Total genomic DNA from the EBN samples was extracted using the CTAB method, SDS method, a buccal swab DNA extraction kit (kit method) and the guanidine isothiocyanate method ( Attitalla, 2011 ; Kampmann et al, 2016 ; Green and Sambrook, 2018 ; Xia et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR

Fan

Zhong

et al. 2021

Front. Genet.

View full text Add to dashboard Cite

Edible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic information of EBN, a key part of molecular biology, has yet to be reported in Thailand. It is necessary to explore the genetic information of EBN in Thailand based on a quick and simple method to help protect the rights and interests of consumers. This research aimed to systematically evaluate different methods of extracting EBN DNA to improve the efficiency of the analysis of cytochrome b (Cytb) and NADH dehydrogenase subunit 2 (ND2) gene sequences, the establishment of phylogenetic trees, and the genetic information of EBN in Thailand. Additionally, we aimed to develop a quick and simple method for identifying EBN from different species based on the genetic information and amplification-refractory mutation system PCR (ARMS-PCR). By comparing the four methods [cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), kit and guanidinium isothiocyanate methods] for EBN extraction, we found that the guanidinium isothiocyanate method was the optimal extraction method. Phylogenetic trees generated on the basis of Cytb and ND2 gene analyses showed that 26 samples of house EBN and 4 samples of cave EBN came from Aerodramus fuciphagus and Aerodramus maximus, respectively. In addition, to distinguish different samples from different species of Apodiformes, we designed 4 polymerase chain reaction (PCR) amplification primers based on the ND2 gene sequences of A. fuciphagus and A. maximus. The ARMS-PCR results showed band lengths for A. fuciphagus EBN of 533, 402, and 201 bp, while those for A. maximus EBN were 463, 317, and 201 bp. Collectively, the results showed that ARMS-PCR is a fast and simple method for the genetic identification of EBN based on designing specific original identification primers.

show abstract

Modified CTAB Method for High Quality Genomic DNA Extraction from Medicinal Plants

Cited by 48 publications

References 11 publications

Temporal and spatial pattern of endophytic fungi diversity of Camellia sinensis (cv. Shu Cha Zao)

Temporal and spatial pattern of endophytic fungi diversity of Camellia sinensis (cv. Shu Cha Zao)

Comparison of methods to extract PCR-amplifiable DNA from fruit, herbal and black teas

Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR

Contact Info

Product

Resources

About