Modified cell-electrospinning for 3D myogenesis of C2C12s in aligned fibrin microfiber bundles

Guo, Yanheng; Gilbert-Honick, Jordana; Somers, Sarah M.; Mao, Hai Quan; Grayson, Warren L.

doi:10.1016/j.bbrc.2019.06.082

Cited by 53 publications

(52 citation statements)

References 17 publications

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“…Therefore, imaging is considered the most efficient preliminary tool to assess cell alignment and myotube formation. A number markers can be used, but the most common myogenic marker for IF is myosin heavy chain (MHC) [ 63 , 68 , 70 , 76 , 83 , 85 , 88 , 89 , 97 , 101 ]. Once myotubes are labeled with MHC, myotubes can be imaged, and fusion index (percent differentiation) can be calculated by dividing the number of nuclei (stained with a nuclear stain) colocated in MHC + myotubes by the total number of nuclei in the image field.…”

Section: Methods For Assessing Skeletal Muscle Regenerationmentioning

confidence: 99%

“…Other myogenic markers that are commonly used to visualize and assess myotube formation, include desmin [ 82 , 103 ] and α-sarcomeric actin [ 82 , 85 , 88 , 89 , 101 ]. In addition, a cytoskeletal actin stain can be utilized to assess overall biomaterial-guided cell alignment [ 50 , 70 , 72 , 76 , 84 , 87 , 98 ].…”

Section: Methods For Assessing Skeletal Muscle Regenerationmentioning

confidence: 99%

“…In a similar fashion, Guo et al. developed a cell electrospinning system that encapsulated cell aggregates into a fibrin/PEO copolymer to form highly aligned cell-laden microfiber bundles [ 76 ]. The authors used a wet electrospinning rotating collection bath filled with thrombin and CaCl 2 to crosslink the cell-laden polymer fibers in situ .…”

Section: Biomaterials Therapiesmentioning

confidence: 99%

See 2 more Smart Citations

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

Smoak

Mikos

2020

Materials Today Bio

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Repair of injured skeletal muscle is a sophisticated process that uses immune, muscle, perivascular, and neural cells. In acute injury, the robust endogenous repair process can facilitate complete regeneration with little to no functional deficit. However, in severe injury, the damage is beyond the capacity for self-repair, often resulting in structural and functional deficits. Aside from the insufficiencies in muscle function, the aesthetic deficits can impact quality of life. Current clinical treatments are significantly limited in their capacity to structurally and functionally repair the damaged skeletal muscle. Therefore, alternative approaches are needed. Biomaterial therapies for skeletal muscle engineering have leveraged natural materials with sophisticated scaffold fabrication techniques to guide cell infiltration, alignment, and differentiation. Advances in biomaterials paired with a standardized and rigorous assessment of resulting tissue formation have greatly advanced the field of skeletal muscle engineering in the last several years. Herein, we discuss the current trends in biomaterials-based therapies for skeletal muscle regeneration and present the obstacles still to be overcome before clinical translation is possible. With millions of people affected by muscle trauma each year, the development of a therapy that can repair the structural and functional deficits after severe muscle injury is pivotal.

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Section: Methods For Assessing Skeletal Muscle Regenerationmentioning

confidence: 99%

Section: Methods For Assessing Skeletal Muscle Regenerationmentioning

confidence: 99%

Section: Biomaterials Therapiesmentioning

confidence: 99%

See 1 more Smart Citation

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

Smoak

Mikos

2020

Materials Today Bio

View full text Add to dashboard Cite

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“…Myogenesis was supported as well as an increase in the number of myotube‐associated nuclei, myotube length, and myotube diameter after one week. [ 53 ] This strategy may be useful for stem cells.…”

Section: Topographymentioning

confidence: 99%

The Effect of Physical Cues of Biomaterial Scaffolds on Stem Cell Behavior

Wang

Hashemi

Stratton

et al. 2020

Adv Healthcare Materials

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Stem cells have been sought as a promising cell source in the tissue engineering field due to their proliferative capacity as well as differentiation potential. Biomaterials have been utilized to facilitate the delivery of stem cells in order to improve their engraftment and long-term viability upon implantation. Biomaterials also have been developed as scaffolds to promote stem cell induced tissue regeneration. This review focuses on the latter where the biomaterial scaffold is designed to provide physical cues to stem cells in order to promote their behavior for tissue formation. Recent work that explores the effect of scaffold physical properties, topography, mechanical properties and electrical properties, is discussed. Although still being elucidated, the biological mechanisms, including cell shape, focal adhesion distribution, and nuclear shape, are presented. This review also discusses emerging areas and challenges in clinical translation.

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“…Therefore, they usually present good cellular attachment, improve cellular behavior, and avoid immunological reactions, although in some cases, these properties are limited due to batch variability within production and purification processes. The most common natural polymers used in biomedical applications include polysaccharides (e.g., alginate [5][6][7], hyaluronic acid [3,8], and chitosan [9,10]), proteins (e.g., collagen [11], silk [12,13], gelatin [14][15][16], and fibrin [17]), and bacterial polyesters (e.g., bacterial cellulose [18]). However, the poor mechanical strength of natural polymers frequently makes the manipulation and biofabrication process difficult.…”

Section: Introductionmentioning

confidence: 99%

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

2021

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Natural polymers have been widely used for biomedical applications in recent decades. They offer the advantages of resembling the extracellular matrix of native tissues and retaining biochemical cues and properties necessary to enhance their biocompatibility, so they usually improve the cellular attachment and behavior and avoid immunological reactions. Moreover, they offer a rapid degradability through natural enzymatic or chemical processes. However, natural polymers present poor mechanical strength, which frequently makes the manipulation processes difficult. Recent advances in biofabrication, 3D printing, microfluidics, and cell-electrospinning allow the manufacturing of complex natural polymer matrixes with biophysical and structural properties similar to those of the extracellular matrix. In addition, these techniques offer the possibility of incorporating different cell lines into the fabrication process, a revolutionary strategy broadly explored in recent years to produce cell-laden scaffolds that can better mimic the properties of functional tissues. In this review, the use of 3D printing, microfluidics, and electrospinning approaches has been extensively investigated for the biofabrication of naturally derived polymer scaffolds with encapsulated cells intended for biomedical applications (e.g., cell therapies, bone and dental grafts, cardiovascular or musculoskeletal tissue regeneration, and wound healing).

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Modified cell-electrospinning for 3D myogenesis of C2C12s in aligned fibrin microfiber bundles

Cited by 53 publications

References 17 publications

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

The Effect of Physical Cues of Biomaterial Scaffolds on Stem Cell Behavior

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

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