The Effect of Physical Cues of Biomaterial Scaffolds on Stem Cell Behavior

Wang, Shuo; Hashemi, Sharareh; Stratton, Scott; Arinzeh, Treena Livingston

doi:10.1002/adhm.202001244

Cited by 51 publications

(35 citation statements)

References 222 publications

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“…Besides mechanical characteristics, another variable that can influence cell response is the scaffold’s topography ( Wang et al, 2021 ). In this case we observed that the rugged surface of the PLA discs seemed to influence how the expanded CD133 + cells organized themselves on the disc, causing them to follow the orientation of the PLA filaments.…”

Section: Discussionmentioning

confidence: 99%

“…The strategy of combining biomaterial scaffolds with cells can be directed to various tissue injuries depending on the cell types applied. One of the most explored cell types in tissue engineering is the mesenchymal stromal cell (MSC), due to its differentiation potential, various isolation sources, low immunogenicity and high immunomodulatory potential ( Li et al, 2019 ; Cun and Hosta-Rigau, 2020 ; Wang et al, 2021 ). Along with MSCs, endothelial cells and their progenitors, such as the expanded CD133 + cell, are also crucial to tissue engineering due to their angiogenic potential ( Bongiovanni et al, 2014 ) and paracrine activity ( Angulski et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

Biagini

Senegaglia

Pereira

et al. 2021

Front. Bioeng. Biotechnol.

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Tissue engineering is a branch of regenerative medicine, which comprises the combination of biomaterials, cells and other bioactive molecules to regenerate tissues. Biomaterial scaffolds act as substrate and as physical support for cells and they can also reproduce the extracellular matrix cues. Although tissue engineering applications in cellular therapy tend to focus on the use of specialized cells from particular tissues or stem cells, little attention has been paid to endothelial progenitors, an important cell type in tissue regeneration. We combined 3D printed poly(lactic acid) scaffolds comprising two different pore sizes with human adipose-derived stromal cells (hASCs) and expanded CD133+ cells to evaluate how these two cell types respond to the different architectures. hASCs represent an ideal source of cells for tissue engineering applications due to their low immunogenicity, paracrine activity and ability to differentiate. Expanded CD133+ cells were isolated from umbilical cord blood and represent a source of endothelial-like cells with angiogenic potential. Fluorescence microscopy and scanning electron microscopy showed that both cell types were able to adhere to the scaffolds and maintain their characteristic morphologies. The porous PLA scaffolds stimulated cell cycle progression of hASCs but led to an arrest in the G1 phase and reduced proliferation of expanded CD133+ cells. Also, while hASCs maintained their undifferentiated profile after 7 days of culture on the scaffolds, expanded CD133+ cells presented a reduction of the von Willebrand factor (vWF), which affected the cells’ angiogenic potential. We did not observe changes in cell behavior for any of the parameters analyzed between the scaffolds with different pore sizes, but the 3D environment created by the scaffolds had different effects on the cell types tested. Unlike the extensively used mesenchymal stem cell types, the 3D PLA scaffolds led to opposite behaviors of the expanded CD133+ cells in terms of cytotoxicity, proliferation and immunophenotype. The results obtained reinforce the importance of studying how different cell types respond to 3D culture systems when considering the scaffold approach for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

Biagini

Senegaglia

Pereira

et al. 2021

Front. Bioeng. Biotechnol.

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“…They provide mechanical support for the proliferating cells and enable them to create newly formed 3D tissue by directing cell growth. For in vivo application, the material used for the scaffolding has to meet several requirements, most importantly biocompatibility and biodegradability 1 – 3 . The importance of biodegradation is underlined with the fact that this process enables tissue remodeling.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Characterization of Poly(Lactic Acid)/ Poly(Hydroxybutyrate)/ Thermoplastic Starch Blends for Tissue Engineering Application

et al. 2021

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Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.

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“…One of the most sought after effects in regenerative medicine is the ability to direct stem cells (SCs) differentiation into specific lineages, a process known to be strongly influenced by the cells' three-dimensional environment [276,277]. Cell (or even organoid [278]) encapsulation in various hydrogel formulations is the dominant approach to study the differentiation of induced pluripotent (iPSCs), MSCs or embryonic SCs (ESCs).…”

Section: Future Trends I: Cellularized Materials In Tissue Engineeringmentioning

confidence: 99%

Colonization versus encapsulation in cell-laden materials design: porosity and process biocompatibility determine cellularization pathways

Parisi

Qin

Fernandes

2021

Phil. Trans. R. Soc. A.

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Seeding materials with living cells has been—and still is—one of the most promising approaches to reproduce the complexity and the functionality of living matter. The strategies to associate living cells with materials are limited to cell encapsulation and colonization, however, the requirements for these two approaches have been seldom discussed systematically. Here we propose a simple two-dimensional map based on materials' pore size and the cytocompatibility of their fabrication process to draw, for the first time, a guide to building cellularized materials. We believe this approach may serve as a straightforward guideline to design new, more relevant materials, able to seize the complexity and the function of biological materials. This article is part of the theme issue ‘Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)’.

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The Effect of Physical Cues of Biomaterial Scaffolds on Stem Cell Behavior

Cited by 51 publications

References 222 publications

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

In Vitro Characterization of Poly(Lactic Acid)/ Poly(Hydroxybutyrate)/ Thermoplastic Starch Blends for Tissue Engineering Application

Colonization versus encapsulation in cell-laden materials design: porosity and process biocompatibility determine cellularization pathways

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