Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells

Abstract

Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer-Emmett-Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett-Joyner-Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film. Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95-53% after 24h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.

Bioluminescent nanopaper for rapid screening of toxic substances

“…xylinum has showed up as one of those emerging materials for cell/tissue engineering of stem cells [24,31], tumor [25] and cartilage [32][33]. As we first report here, bacterial nanocellulose has exhibited its outstanding biocompatibility with marine bacteria A. fischeri, which realized normal proliferation of bacteria on an abundant, low-cost substrate with stable genetic expression.…”

Assessment of the bactericidal effect of green synthesized silver nanoparticles against a panel of infectious microorganisms

“…In order to adequately assess the potential of the synthesised nanoparticles as antimicrobials it is essential to also assess their cytotoxicity against human cells. Thus, we also evaluated the cytotoxicity of our synthesised nanoparticles against a human colon adenocarcinoma cell line (HT-29) (Cacicedo et al, 2016), a human breast cancer cell line (MCF-7) (Nune et al, 2009) and a non-tumorigenic breast epithelial cell line (MCF-12A) (Vorster et al, 2012).…”

Strontium delivery systems based on bacterial cellulose and hydroxyapatite for guided bone regeneration

“…The sample without Ca, BC/SrAp, shows a surface enrichment of Sr and according to NMR results part of Sr is forming Sr-phosphate without HA structure. Bhatnagar (1967), Cacicedo et al (2016), Rehman and Bonfield (1997), Yukhnevich (1973) Wavenumber ( Adsorption studies with the hybrid BC/CaHA/Sr…”