MODIFICATIONS OF RECEPTOR CONCENTRATIONS FOR ADRENALINE, STEROID HORMONES, PROSTAGLANDIN F2αAND GONADOTROPINS IN HYPOPHYSIS AND OVARY OF DAIRY COWS WITH OVARIAN CYSTS

Odore, R.; Badino, P.; Donn, Anthony; Vigo, D.; Biolatti, B.; Girardi, C.

doi:10.1006/phrs.1998.0442

Cited by 22 publications

(18 citation statements)

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“…Hormonal binding assays suggest that the amounts of FSHR and LHCGR in granulosa cells of cysts are lower than those in normal antral follicles (Kawate et al 1990). However, studies using the same binding assay, but with a different sample preparation, have shown that LHCGR and FSHR concentrations in the follicular cysts are similar to those observed in control follicles (Odore et al 1999). Calder et al (2001) compared LHCGR and FSHR mRNA expression by in situ hybridization in ovaries from cows with dominant and non-dominant ovarian follicular cysts and in healthy dominant follicles and found that LHCGR mRNA expression was higher in granulosa cells of dominant follicular cysts than in dominant follicles, without differences in theca cells, and without differences in FSHR mRNA expression.…”

Section: Expression Of Gonadotropin Receptorsmentioning

confidence: 92%

“…The distribution pattern and expression levels of gonadotropin receptors in healthy ovaries have been evaluated by several methods, including conventional and real-time RT-PCR, northern blot assays, and in situ hybridization (Ireland & Roche 1983, Xu et al 1995, Bodensteiner et al 1996, Rajapaksha et al 1996, Bao et al 1997, Evans & Fortune 1997, Bao & Garverick 1998, Soumano et al 1998, Odore et al 1999, Calder et al 2001, Manikkam et al 2001, Robert et al 2003, Braw-Tal & Roth 2005, Luo & Wiltbank 2006, Mihm et al 2006, Nogueira et al 2007, Nimz et al 2009). In recent studies conducted in our laboratory, we analyzed the relative expression levels of LHCGR and FSHR in follicular cysts of cows with COD and antral follicles of healthy animals by real-time RT-PCR and, in agreement with that found by other authors, we found that mRNA expression of FSHR in antral follicles was exclusively localized to granulosa cells and it decreased as follicle size increased, and that LHCGR mRNA was detected in theca cells of antral follicles of all sizes and in the granulosa cells of large antral follicles, being theca cells of medium antral follicles, the ones that showed the highest expression (Marelli et al 2014).…”

Section: Expression Of Gonadotropin Receptorsmentioning

confidence: 99%

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Molecular aspects of bovine cystic ovarian disease pathogenesis

Ortega¹,

Marelli²,

Rey³

et al. 2015

Reproduction

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Cystic ovarian disease (COD) is one of the main causes of reproductive failure in cattle and causes severe economic loss to the dairy farm industry because it increases both days open in the post partum period and replacement rates due to infertility. This disease is the consequence of the failure of a mature follicle to ovulate at the time of ovulation in the estrous cycle. This review examines the evidence for the role of altered steroid and gonadotropin signaling systems and the proliferation/apoptosis balance in the ovary with cystic structures. This evidence suggests that changes in the expression of ovarian molecular components associated with these cellular mechanisms could play a fundamental role in the pathogenesis of COD. The evidence also shows that gonadotropin receptor expression in bovine cystic follicles is altered, which suggests that changes in the signaling system of gonadotropins could play a fundamental role in the pathogenesis of conditions characterized by altered ovulation, such as COD. Ovaries from animals with COD exhibit a disrupted steroid receptor pattern with modifications in the expression of coregulatory proteins. These changes in the pathways of endocrine action would trigger the changes in proliferation and apoptosis underlying the aberrant persistence of follicular cysts. Free Spanish abstract: A Spanish translation of this abstract is freely available at

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Section: Expression Of Gonadotropin Receptorsmentioning

confidence: 92%

Section: Expression Of Gonadotropin Receptorsmentioning

confidence: 99%

Molecular aspects of bovine cystic ovarian disease pathogenesis

Ortega¹,

Marelli²,

Rey³

et al. 2015

Reproduction

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“…However, this hypothesis is not supported by data from Calder et al [80] showing that ER-β mRNA expression was not altered in growing young cysts. Odore et al [79] did, however, find decreased oestrogen receptor concentrations in follicular cysts, but the oestrogen receptor type was not defined.…”

Section: Ovarian/follicular Dysfunctionmentioning

confidence: 95%

“…The LH surge initiates a complex multi-gene, multistep process in which timing is essential, finally leading to ovulation of the pre-ovulatory follicle [77]. According to Kawate et al [78], FSH and LH receptor numbers in granulosa cells of cysts are decreased when compared to normal follicles, but this is contradicted by data from Odore et al [79] and Calder et al [80]. Discrepancies between studies may be explained by differences in methodology such as demonstration of the receptor itself or its mRNA, and the division of cysts into oestrogenactive and oestrogen-inactive.…”

Section: Ovarian/follicular Dysfunctionmentioning

confidence: 99%

Aetiology and pathogenesis of cystic ovarian follicles in dairy cattle: a review

2006

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-Cystic ovarian follicles (COF) are an important ovarian dysfunction and a major cause of reproductive failure in dairy cattle. Due to the complexity of the disorder and the heterogeneity of the clinical signs, a clear definition is lacking. A follicle becomes cystic when it fails to ovulate and persists on the ovary. Despite an abundance of literature on the subject, the exact pathogenesis of COF is unclear. It is generally accepted that disruption of the hypothalamo-pituitary-gonadal axis, by endogenous and/or exogenous factors, causes cyst formation. Secretion of GnRH/LH from the hypothalamus-pituitary is aberrant, which is attributed to insensitivity of the hypothalamus-pituitary to the positive feedback effect of oestrogens. In addition, several factors can influence GnRH/LH release at the hypothalamo-pituitary level. At the ovarian level, cellular and molecular changes in the growing follicle may contribute to anovulation and cyst formation, but studying follicular changes prior to cyst formation remains extremely difficult. Differences in receptor expression between COF and dominant follicles may be an indication of the pathways involved in cyst formation. The genotypic and phenotypic link of COF with milk yield may be attributed to negative energy balance and the associated metabolic and hormonal adaptations. Altered metabolite and hormone concentrations may influence follicle growth and cyst development, both at the level of the hypothalamus-pituitary and the ovarian level.cystic ovarian follicles / pathogenesis / hypothalamus-pituitary / ovary / negative energy balance

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“…The pre-ovulatory surge of luteinizing hormone (LH) is either blocked, insufficient in magnitude or mistimed during dominant follicle maturation; anovulation and cyst formation is the result (Vanholder et al, 2006). Other pathogenic pathways related to ovarian factors have been also suggested; alterations in expression of LH, FSH receptors (Kawate et al, 1990), and estradiol receptor β (Odore et al, 1999) was noted in granulosa cells of cysts compared to normal follicles. COF is associated with great losses to dairy business, it leads to longer calving-calving intervals, delayed postpartum conception, increased number of inseminations per conception (Hooijer et al, 2001), and increased culling rate (Grohn et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Reproductive Performance of Holstein-Friesian Dairy Cows Subjected to Different Treatments for Cystic Ovarian Follicles

Ismail¹,

Waheeb²,

Hatab³

2017

AJVS

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Key words: cystic follicles, estrus, GnRH, PGF2α, Potassium iodide.Cystic ovarian follicles "COF" is a common ovarian disorder diagnosed in dairy cattle that cause significant economic losses in dairy industry. This study was designed to evaluate efficacy of different therapeutic protocols of COF without determining cyst type. The study was conducted on 104 Holstein-Friesian cystic cows that were divided randomly to the following treatments: Experiment Ι: 1) Group G, n=27, subdivided into A) n=13: treated with 20μg Buserelin-GnRH agonist and B) n=14: treated with 100μg GonadorelinGnRH agonist; 2) Group GP, n=23: received 20μg Buserelin on day 0-500μg of PGF2α on day 10; 3) Group GGP, n=27: received 2 doses of 20μg Buserelin 7 ds apart followed by 500μg PGF2α 7ds from the 2 nd Buserelin injection; 4) Group GPG (Ovsynch), n=17: received 20μg Buserelin on day 0-500μg PGF2α on day 7, and 48hrs later, 20μg Buserelin was injected; 5) Group C, n=10: no treatment received. 22 normal cycling cows were kept as a normal control-NC. Experiment ΙΙ: This part of the study was conducted on 34 cystic animals that did not respond to one of the previous treatments in experiment Ι and were divided into 2 groups: 1) Group CGP, n=19: CIDR+100μg Gonadorelin-(d 0), then 500μg of PGF2α on day 7, insert was removed on day 9; 2) Group PI, n=15: treated by oral administration of 5gm Potassium iodide dissolved in 500 ml sterile water for 7 successive days. Treated Cows were observed for 35 days from the end of treatment, females exhibited estrus were inseminated within 12 hours of detected heat. Pregnancy was confirmed by per rectum on day 50 post-insemination. Results showed that GP protocol achieved highest (P<0.05) estrus induction rate (EIR) recorded at 87%, followed by 70% for G and GGP group, and lowest (53%) obtained in GPG treated cows. Conception rate at 1 st estrus and total conception rate (3 cycles after treatment) did not differ significantly among treatment groups, while pregnancy rates (PR) were significantly different (P<0.05) with highest (65.21%) obtained in GP protocol. Other groups recorded 63%, 55.55%, and 41.17% PR for G, GGP, and GPG respectively. In experiment ΙΙ, EIR was 47.36% and 33.33% for CGP and PI respectively with no significant difference, all conceived after 3 estrous cycles by the end of the experiment. In conclusion, GnRH+ PGF2α 10ds later was the most suitable treatment of COF regardless of cyst type, GGP protocol might be a profitable alternative to Ovsynch for treatment of cystic ovaries. Persistent cases of COF could be recruited to avoid culling for other treatment options such as CIDR or Potassium iodide with equal therapeutic efficacy.Correspondence to: rwaheeb@alexu.edu.eg.

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MODIFICATIONS OF RECEPTOR CONCENTRATIONS FOR ADRENALINE, STEROID HORMONES, PROSTAGLANDIN F2αAND GONADOTROPINS IN HYPOPHYSIS AND OVARY OF DAIRY COWS WITH OVARIAN CYSTS

Cited by 22 publications

References 0 publications

Molecular aspects of bovine cystic ovarian disease pathogenesis

Molecular aspects of bovine cystic ovarian disease pathogenesis

Aetiology and pathogenesis of cystic ovarian follicles in dairy cattle: a review

Reproductive Performance of Holstein-Friesian Dairy Cows Subjected to Different Treatments for Cystic Ovarian Follicles

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