Modifications of Plasma Post-Heparin Lipolytic Activity and Tissue Lipoprotein Lipase Activity Induced in the Rat by Acute Administration of Ethanol or Propan-2-ol

Abstract

1. The oral administration of propan-2-ol [isopropanol; 100 mmol (6 g)/kg body weight] or ethanol [130 mmol (6 g)/kg body weight] to starved rats produced no change in plasma post-heparin lipase activity (PHLA) compared with that observed in 154 mmol/1 sodium chloride (saline)-treated rats. 2. An increase of adipose tissue lipoprotein lipase (LLA) and a decrease of heart LLA occurred in isopropanol-treated animals, whereas no significant changes were found in these activities after ethanol administration. 3. Since administration of isopropanol produces hyperglycaemia, observations were also made in rats receiving glucose infusion rather than saline. In these animals a rise in PHLA and adipose tissue LLA, and a fall in heart LLA, occurred. 4. It is suggested that the changes in tissue LLA produced by isopropanol are mediated by the rise in blood glucose.

Insulin‐like effect of some polyamines on lipoprotein lipase from rat adipose tissue

“…Addition of spermidine (sigma), spermine (Calbiochem) or putrescine (Merck) to the medium was done at zero time. At the end of the incubations, fat pads were quickly removed, homogenized in 0.25 M sucrose (pH 8.1) containing heparin (2 IU/ml) and the heparin eluted lipoprotein lipase activity determined using Intralipid (Vitrum) as substrate, following the experimental conditions previously described [7]. The amount of free fatty acids (FFA) released during 60 rain in each assay (carried out in duplicate) was determined according to Dole and Meinertz [8] and taken as a measure of lipoprotein lipase activity, which was expressed as micromoles of FFA released per hour and per g adipose tissue wet weight.…”