Modifications induced by atherogenic diet in the capacity of the arterial wall in rats to respond to surgical insult

Buján, Julia; Bellón, Juan M.; Sabater, Cristina; Jurado, F.; García‐Honduvilla, Natalio; Domínguez, Belén; Jorge, Eduardo

doi:10.1016/0021-9150(95)05727-7

Cited by 17 publications

(19 citation statements)

References 23 publications

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“…Smooth muscle cells are considered to be the main source of origin of osteogenic cells in calcified vascular lesions [16]. Other data indicate that the occurrence of ectopic medial calcifications is associated with adventitious cells [17]. Vessel and valve endothelial cells serve as a cellular barrier between the blood and the main tissue components, but participate in the endothelial-mesenchymal transition, playing a key role in tissue fibrosis and calcification [18].…”

Section: Discussionmentioning

confidence: 99%

In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

Антонова

Sevost'yanova

Миронов

et al. 2018

Kompleks. probl. serdečno-sosud. zabol.

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25омплексные проблемы сердечно-сосудистых заболеваний К Highlights • The study demonstrates the benefits of using functionalized small-diameter biodegradable vascular graft for de novo vascular tissue formation.• The release of growth factors and chemoattractant molecules into the vascular bed after the graft implantation stimulates neotissue formation and levels out inflammation and calcification. BackgroundCurrently, the search for the bioactive molecules capable of promoting formation of the vascular tissue is still ongoing. We have previously demonstrated that incorporation of the growth factors and chemoattractant molecules into the biodegradable tubular scaffolds can increase their primary patency upon the implantation into rat abdominal aorta. However, further studies are required to investigate tissue remodeling using functionalized vascular grafts with the same diameter as a replaced native vessel. AimTo investigate the specific aspects of de novo vascular tissue formation and calcification employing rat abdominal aorta interposition model and vascular grafts with 1.5 mm diameter with incorporated vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and stromal cell-derived factor (SDF)-1α. MethodsTubular grafts with a diameter of 1.5 mm were blended of poly(3-hydroxybutyrateco-3-hydroxyvalerate) and poly(ε-caprolactone) (PHBV/PCL). Grafts without growth factors were fabricated using standard electrospinning technique whilst grafts with incorporated growth factors were prepared utilizing emulsion electrospinning. VEGF was incorporated into the inner third, whereas bFGF and SDF-1α were incorporated into the outer two-thirds of the graft. Grafts were implanted into the abdominal aortas of Wistar rats for 1, 3, 6, and 12 months following scanning electron microscopy along with histological and immunofluorescent examination. ResultsPrimary patency of the grafts with VEGF, bFGF, and SDF-1α reached 93% indicative of structural integrity of the vascular tissue. Neither signs of inflammation nor severe calcification was detected. ConclusionAs in 2 mm diameter vascular grafts, incorporation of bioactive factors into 1.5 mm diameter grafts increased their long-term primary patency and improved vascular tissue formation in comparison with non-modified grafts.

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Section: Discussionmentioning

confidence: 99%

In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

Антонова

Sevost'yanova

Миронов

et al. 2018

Kompleks. probl. serdečno-sosud. zabol.

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“…Indeed, surgical resection of the adventitia markedly reduces segmental medial artery calcification in a rat model of calcific vasculopathy (43). Such functional spatial relationships would help explain the circumferential pattern of medial artery calcification prevalent in diabetes.…”

Section: Figurementioning

confidence: 98%

Msx2 promotes cardiovascular calcification by activating paracrine Wnt signals

Shao¹,

Cheng²,

et al. 2005

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“…14 and data not shown); thus, some diet-regulated metabolic parameter, potentially a phospholipid or oxidized sterol (6,8,51), synergizes with Msx2-Wnt signaling to drive medial calcification. Of note, surgical resection of the adventitia reduces segmental medial artery calcification (43); whether this arises from loss of adventitial pro-osteogenic paracrine signals, reductions in the vascular osteoprogenitor pool, or a combination of both is unknown. Cell surface markers that stratify vascular myofibroblast stages of development are currently lacking, but will be necessary to identify precisely how Msx2-Wnt signaling controls the fate of vascular mesenchymal cells.…”

Section: Figurementioning

confidence: 99%

“…High-fat diets upregulate aortic BMP2 expression (10). In addition to providing progenitors for medial disease processes (40,41,43), Msx2-expressing adventitial myofibroblasts produce an osteogenic milieu via induction of Wnt agonists and inhibition of the LRP5/6 antagonist, Dkk1. This causes nuclear localization and activation of β-catenin -an indispensable coregulator of osteoblast differentiation and mineralization (24); as reflected by TCF/LEF-dependent transcription, activation occurs in a subset of medial CVCs.…”

Section: Figure 11mentioning

confidence: 99%

“…This causes nuclear localization and activation of β-catenin -an indispensable coregulator of osteoblast differentiation and mineralization (24); as reflected by TCF/LEF-dependent transcription, activation occurs in a subset of medial CVCs. CVCs of Demer (50), perhaps derived from migratory adventitial progenitors (10,40,41,43), respond by upregulating ALP expression via key osteogenic transcription factors (11). Vascular PTH1R signaling dampens Msx2-Wnt signaling.…”

Section: Figure 11mentioning

confidence: 99%

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