In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

Антонова, Л. В.; Sevost'yanova, V. V.; Миронов, А. В.; Кривкина, Е. О.; Великанова, Е. А.; Матвеева, В. Г.; Глушкова, Т. В.; Elgudin, Y.L.; Барбараш, Л. С.

doi:10.17802/2306-1278-2018-7-2-25-36

Cited by 19 publications

(19 citation statements)

References 25 publications

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“…Interestingly, the rates of PHBV/PCL graft resorption in sheep exceed those reported in the literature, that is 3-4 years for PCL and at least 5 months for PHBV [16,17]. In our previous studies, the degradation of PHBV/PCL grafts implanted into rats was also much longer as compared to this experiment [14,15]. In this study, PHBV/PCL-GFmix Hep/Ilo grafts completely degraded only 6 months postoperation.…”

Section: Resultscontrasting

confidence: 47%

“…Using a rat model of abdominal aorta implantation, we showed that immobilization of angiogenic factors on graft luminal surface led to a rapid repopulation of PHBV/PCL grafts by endothelial progenitor cells [14,15]. Three months following implantation in rats, we observed a complete re-endothelialization along with formation of neointima and the presence of smooth muscle cells producing collagen types I and IV [14,15]. The primary patency of such grafts was 93.3% 12 months following implantation.…”

Section: Introductionmentioning

confidence: 87%

“…Previously, our group developed a small caliber electrospun biodegradable graft based on a polyhydroxybutyrate/valerate (PHBV) and polycaprolactone (PCL) blend, additionally modified by various pro-angiogenic factors [13,14]. Using a rat model of abdominal aorta implantation, we showed that immobilization of angiogenic factors on graft luminal surface led to a rapid repopulation of PHBV/PCL grafts by endothelial progenitor cells [14,15]. Three months following implantation in rats, we observed a complete re-endothelialization along with formation of neointima and the presence of smooth muscle cells producing collagen types I and IV [14,15].…”

Section: Introductionmentioning

confidence: 99%

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A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep

et al. 2020

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The development of novel biodegradable vascular grafts of a small diameter (<6 mm) is an unmet clinical need for patients requiring arterial replacement. Here we performed a pre-clinical study of new small-caliber biodegradable vascular grafts using a sheep model of carotid artery implantation. The 4 mm diameter vascular grafts were manufactured using a mix of polyhydroxybutyrate/valerate and polycaprolactone supplemented with growth factors VEGF, bFGF and SDF-1α (PHBV/PCL-GFmix) and additionally modified by a polymer hydrogel coating with incorporation of drugs heparin and iloprost (PHBV/PCL-GFmixHep/Ilo). Animals with carotid artery autograft implantation and those implanted with clinically used GORE-TEX® grafts were used as control groups. We observed that 24 h following surgery, animals with carotid artery autograft implantation showed 87.5% patency, while all the PHBV/PCL-GFmix and GORE-TEX® grafts displayed thrombosis. PHBV/PCL-GFmixHep/Ilo grafts demonstrated 62.5% patency 24 h following surgery and it had remained at 50% 1 year post-operation. All the PHBV/PCL grafts completely degraded less than 1 year following surgery and were replaced by de novo vasculature without evidence of calcification. On the other hand, GORE-TEX® grafts displayed substantial amounts of calcium deposits throughout graft tissues. Thus, here we report a potential clinical usefulness of PHBV/PCL grafts upon their additional modification by growth factors and drugs to promote endothelialization and reduce thrombogenicity.

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Section: Resultscontrasting

confidence: 47%

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep

et al. 2020

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“…биодеградируемых полимеров [2,3]. При этом дополнительное инкорпорирование в состав полимерных сосудистых матриксов (графтов) биологически активных веществ (ростовых факторов и хемоаттрактантов) стимулирует привлечение и дифференцировку эндогенных прогениторных клеток in situ [4,5].…”

Section: Resultsunclassified

Biodegradable Vascular Graft Reinforced With a Biodegradable Sheath

Антонова

Кривкина

Резвова

et al. 2019

Kompleks. probl. serdečno-sosud. zabol.

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Background. Tissue-engineered vascular grafts can be reinforced by a biostable or biodegradable polymer sheath. A combination of electrospinning, routinely used for fabrication of biodegradable tubular grafts, and the layer-by-layer coating allows forming a polymeric sheath ensuring long-term integrity and high biocompatibility of the vascular grafts after the implantation. Aim To evaluate mechanical properties and in vivo performance of biodegradable small-diameter vascular grafts with a reinforcing sheath.Methods. Tubular grafts (4 mm diameter) were fabricated from poly(3-hydroxybutyrate-co3-hydroxyvalerate) and poly(ε-caprolactone) by emulsion electrospinning with the incorporation of vascular endothelial growth factor (VEGF) into the inner third of the graft and basic fibroblast growth factor (bFGF) along with stromal cell-derived factor-1α (SDF-1α) into the outer two thirds of the graft wall. Poly(ε-caprolactone) sheath was formed by the layer-by-layer coating. Upon graft fabrication, scanning electron microscopy was performed to assess the grafts’ surface, tensile testing allowed evaluating mechanical properties. The samples were implanted into the ovine carotid artery (n = 5 animals) for 12 months with the subsequent histological examination.Results. Sintering temperature of 160°C during the extrusion allowed effective and delicate merging of poly(ε-caprolactone) coating with the outer surface of the poly(3hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) tubular graft. The thickness of poly(ε-caprolactone) fiber was 380–400 μm, the increment of the reinforcing filament was 1 mm. The reinforcing sheath led to a 3-fold increase in durability and elastic modulus of the vascular grafts. At the 12-months follow-up, the grafts reported retained integrity. No signs of inflammation or calcification were found.Conclusion. The poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(ε-caprolactone) vascular grafts with hierarchically incorporated growth factors and the reinforced poly(ε-caprolactone) spiral sheath demonstrated improved mechanical properties while retaining integrity and high biocompatibility after the long-term implantation into the ovine carotid artery.

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“…Доказано, что молекулы bFGF, SDF-1α поддержали устойчивое VEGF-индуцированное образование качественной эндотелиальной выстилки на внутренней поверхности сосудистого протеза. Высокая первичная проходимость в период 12-месячной имплантации крысам обеспечила формирование сосудистых тканей на месте биодеградируемого матрикса с одновременным снижением интенсивности кальцификации и отсутствием признаков иммунного отторжения [73].…”

Section: основные биологически активные вещества используемые для моunclassified

Biodegradable small-diameter vascular graft: types of modification with bioactive molecules and RGD peptides

Senokosova

Кривкина

Антонова

et al. 2020

RJTAO

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The need for small-diameter grafts for replacing the damaged area of the blood pool is still very high. These grafts are very popular for coronary artery bypass grafting. Polymeric synthetic grafts are an alternative to autografts. A promising area of tissue engineering is the creation of a biodegradable graft. It can serve as the basis for de novo generation of vascular tissue directly in the patient’s body. Optimization of the polymer composition of products has led to improved physicomechanical and biocompatible properties of the products. However, the improvements are still far from needed. One of the decisive factors in the reliability of a small-diameter vascular graft is the early formation of endothelial lining on its inner surface, which can provide atrombogenic effect and full lumen of the future newly formed vessel. To achieve this goal, grafts are modified by incorporating bioactive molecules or functionally active peptide sequences into the polymer composition or immobilizing on its inner surface. Peptide sequences include cell adhesion site – arginine-glycine-aspartic acid (RGD peptide). This sequence is present in most extracellular matrix proteins and has a tropism for integrin receptors of endothelial cells. Many studies have shown that imitation of the functional activity of the natural extracellular matrix can promote spontaneous endothelization of the inner surface of a vascular graft. Moreover, configuration of the RGD peptide determines the survival and differentiation of endothelial cells. The linker through which the peptide is crosslinked to the polymer surface determines the bioavailability of the RGD peptide for endothelial cells.

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In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

Cited by 19 publications

References 25 publications

A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep

A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep

Biodegradable Vascular Graft Reinforced With a Biodegradable Sheath

Biodegradable small-diameter vascular graft: types of modification with bioactive molecules and RGD peptides

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