Modification of the passive heamagglutination test for detection of <i>infectious bursal disease virus</i>

Abstract

ABSTRACT

Direct passive hemagglutination test for rapid quantification of plasma load of the <i>Human Immunodeficiency Virus</i>

“…To apply PHA for quantitative detection of viruses, we [5]. This modification of the passive hemagglutination test can be used to detect viruses, from specimens that contain only the virus while the known antibodies are in sera.…”

“…In the passive hemagglutination test used to detect antibodies and in the Modified Passive Hemagglutination test developed for virus titers [5], either the antigen or the positive serum (antibodies) is known. So, a positive reaction identifies the unknown as homologous complement of either the known antigen or the known antibody.…”

“…So it can be used to detect both the antibodies and the antigens. By using serial dilutions of viral antigens to sensitize red blood cells, before reacting them onto known homologous antibodies, we have been able to develop a Modified Passive Hemagglutination Test and successfully used it for quantitative detection of Infectious Bursa Disease Virus [5]. It was therefore thought achievable to develop a direct passive hemagglutination (DPHA) test for rapid assessment of HIV titers in plasmas, since the antigens and their homologous antibodies coexist at high concentrations in the specimen.…”

Aluminum-Magnesium Silicate enhances release of virions of cultured <i>Fowl Pox Virus</i> and inhibits the virus

“…Four ml of each of the reconstituted vaccines was mixed with equal volume (4 g) of The Synthetic AMS [10] and kept at room temperature for one hour. The mixtures were then centrifuged for 10 minutes, at 2000 revolutions per minute.…”

“…The Synthetic AMS got from reaction of Aluminum silicate and Magnesium silicate [10] has exhibited antiviral effects against all four viral families, so far tested, including, Paramyxoviridae, Birnaviridae, Parvoviridae and Othomyxoviridae [10]. A need therefore exists to test it against other viral families.…”