Modification of the n-6/n-3 fatty acid ratio in the phospholipids of rat ventricular myocytes in culture by the use of synthetic media: Functional and biochemical consequences in normoxic and hypoxic conditions

Grynberg, Alain; Fantini, Elisabeth; Athias, Pierre; Degois, Martine; Guenot, L.; Courtois, Martine; Khatami, Shohreh

doi:10.1016/s0022-2828(88)80141-x

Cited by 41 publications

(21 citation statements)

References 39 publications

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“…Conversely, supplementation with EPA induced a high level in EPA (12.9%) but also in C22 : 5 n-3 (14.9%), indicating a rapid elongation of C20:5 n-3. Interestingly, the DHA level remained low in this cell group, underlining previous reports which showed that the delta-4 desaturase activity is low in heart muscle cells [5,6,23], No other major difference in fatty acid composition appeared between the EPAMed or DHAMed grown cardiomyocytes. In control conditions both PC-PLA1 and PC-PLA2 specific activities were significantly higher in DHA rich cells than in EPA rich cells (+ 23% for PC-PLA1 and + 17% for PC-PLA2).…”

Section: Resultssupporting

confidence: 86%

“…After mincing the hearts, the cardiac cells were dissociated through seven proteolytic treatments in 0.1% trypsin (Difco) at 30 ° C. Myocyte enrichment was performed using a two-step (30 and 120 min) differential attachment procedure [5]. The cells were resuspended in standard culture medium.…”

Section: Culture Preparation and Handlingmentioning

confidence: 99%

“…Numerous studies demonstrated that a modification of the fatty acid intake resuits in large alterations in the heart membrane fatty acid composition [2][3][4][5][6]. On the basis of studies performed on animals [7], human [8] or cultured cells [5], a direct influence of such alterations on cardiac function has been proposed in physiologic as well as in hypoxic conditions. Heart membrane stability is essential to ensure a correct electrophysiological activity under normoxic conditions as well as a satisfactory recovery after ischemic insult and reperfusion.…”

Section: Introductionmentioning

confidence: 99%

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Eicosapentaenoic and docosahexaenoic acids in cultured rat ventricular myocytes and hypoxia-induced alterations of phospholipase?A activity

et al. 1992

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Hypoxia was reported to induce a decrease in phosphatidylcholine-hydrolyzing phospholipase activity (PC-PLA) in cultured rat cardiomyocytes. This work was intended to compare the influence of the presence of either eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) in the phospholipids on the PC-PLA activity in normoxic and hypoxic conditions. The enrichment of the medium with EPA or DHA resulted in cell phospholipids containing about 2% or 22% DHA, respectively. These cells were then submitted for 3.5 h to either normoxia or hypoxia and the PC-PLA activities were assayed using [1-14C] dioleoyl-PC (pH 8.4 for PC-PLA2 and 4.9 for PC-PLA1). The results show that both enzymic activities are significantly higher in DHA-rich cardiomyocytes. Hypoxia induced a significant decrease in PC-PLA2 (about 25%) which was not statistically different between the two groups of cells. The hypoxia-induced decrease in PC-PLA1 was not found significant. In conclusion, the nature of the long chain n-3 polyunsaturated fatty acids in the phospholipids appears to contribute to the regulation of PC-PLA activity but not to influence its decrease during hypoxia.

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Section: Resultssupporting

confidence: 86%

Section: Culture Preparation and Handlingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Eicosapentaenoic and docosahexaenoic acids in cultured rat ventricular myocytes and hypoxia-induced alterations of phospholipase?A activity

et al. 1992

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“…This model has been a good tool to study related cardiovascular problems, for instance, experiments on arrhythmogenic drugs, ischemia, hypoxia etc. [23,24,25,26,27]. EP signals have a close correlation of the depolarization and repolarizing phase of the action potentials ( Figure 1a).…”

Section: Cardiac Cells Culturementioning

confidence: 84%

In vitroarrhythmia generation by mild hypothermia: a pitchfork bifurcation type process

Xu¹,

Jacquir²,

Laurent³

et al. 2015

Physiol. Meas.

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The neurological damage after cardiac arrest (CA) constitutes a big challenge of hospital discharge.The therapeutic hypothermia (34 • C − 32 • C) has shown its benefit to reduce cerebral oxygen demand and improve neurological outcomes after the cardiac arrest. However, it can have many adverse effects, The process of therapeutic hypothermia after cardiac arrest can be represented by a Pitchfork bifurcation type process, which could explain the different ratio of arrhythmia among the adverse effects after this therapy. This nonlinear dynamics suggests that a variable speed of cooling / rewarming, especially when passing 35 • C, would help to decrease the ratio of post-hypothermia arrhythmia and then improve the hospital output.

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“…Cette étude a été réalisée pour savoir si une incorporation sélective d'acides gras n-6 ou n-3 dans les phospholipides membranaires responsible for a decrease in the n-6/n-3 ratio in cardiac phospholipids (Rocquelin et al, 1986;Swanson et al, 1986;Hartog et al, 1987;Nalbone et al, 1988). On the other hand, these changes in cellular lipid environment may induce noticeable alterations in cardiac function (Hock et al, 1987;Grynberg et al, 1988;Lamers et al, 1988) …”

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Influence of dietary linseed oil and sunflower seed oil on some mechanical and metabolic parameters of isolated working rat hearts

Demaison

Grynberg²

1991

Reprod. Nutr. Dévelop.

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Summary ― The role played by membrane lipid environment on cardiac function remains poorly defined. The polyunsaturated fatty acid profile of myocardial phospholipids could be of utmost importance in the regulation of key-enzyme activities. This study was undertaken to determine whether selective incorporation of n-6 or n-3 fatty acids in membrane phospholipids might influence cardiac mechanical performances and metabolism. For 8 wk, male weanling Wistar rats were fed a semipurified diet containing either 10% sunflower seed oil (72% C18:2 n-6) or 10% linseed oil (54% C18:3n-3) as the sole source of lipids. The hearts were then removed and perfused according to working mode with a Krebs-Henseleit buffer containing glucose (11 mM) and insulin (10 UII1). Cardiac rate, coronary and aortic flows and ejection fraction were monitored after 30 min of perfusion. Myocardial metabolism was estimated by evaluating the intracellular fate of 1-!4C palmitate. Sunflower seed oil and linseed oil feeding did not modify either coronary or aortic flow, which suggests that cardiac mechanical work was not affected by the diets. Conversely, cardiac rate was significantly decreased (-18%; P < 0.01) when rats were fed the n-3 polyunsaturated fatty acid rich diet. Radioanalysis of the myocardial metabolism suggested that replacing n-6 polyunsaturated fatty acids by n-3 polyunsaturated fatty acids: i) did not alter palmitate uptake; ii) prolonged palmitate incorporation into cardiac triglycerides; iii) reduced beta-oxidation of palmitic acid. These results support the assumption that dietary fatty acids, particularly n-6 and n-3 fatty acids, play an important role in the regulation of cardiac mechanical and metabolic activity.dietary polyunsaturated fatty acid / membrane phospholipid I isolated perfused rat heart I mechanical activity / metabolism

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Modification of the n-6/n-3 fatty acid ratio in the phospholipids of rat ventricular myocytes in culture by the use of synthetic media: Functional and biochemical consequences in normoxic and hypoxic conditions

Cited by 41 publications

References 39 publications

Eicosapentaenoic and docosahexaenoic acids in cultured rat ventricular myocytes and hypoxia-induced alterations of phospholipase?A activity

Eicosapentaenoic and docosahexaenoic acids in cultured rat ventricular myocytes and hypoxia-induced alterations of phospholipase?A activity

In vitroarrhythmia generation by mild hypothermia: a pitchfork bifurcation type process

Influence of dietary linseed oil and sunflower seed oil on some mechanical and metabolic parameters of isolated working rat hearts

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