Modification of plasma membrane NADPH oxidase activity in cucumber seedling roots in response to cadmium stress

Jakubowska, D; Janicka, Małgorzata; Kabała, Katarzyna; Migocka, Magdalena; Reda, Małgorzata

doi:10.1016/j.plantsci.2015.02.005

Cited by 59 publications

(39 citation statements)

References 77 publications

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“…It was reported in salt‐stressed Arabidopsis roots that H 2 S induces H 2 O 2 accumulation by alteration of NADPH oxidase activity (Li et al ). Our earlier work has shown that treatment of cucumber with Cd stimulates the activity of NADPH oxidase as well as induces the expression of several encoding genes (Jakubowska et al ). In this study, exogenous application of NaHS to control plants also caused a significant increase in enzyme activity as well as in transcript levels of some CsRboh genes, confirming that H 2 S can promote H 2 O 2 generation via NADPH oxidase and suggesting that H 2 S‐dependent regulation of H 2 O 2 content may also occur in cucumber subjected to Cd.…”

Section: Discussionmentioning

confidence: 99%

“…NADPH oxidase activity (EC 1.6.3.1) was assayed in plasma membrane fractions according to the method of Sagi and Fluhr () described by Jakubowska et al () using 0.5 mM XTT [2,3‐bis(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide inner salt] and 0.1 mM NADPH.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of melting curve was performed to confirm the specificity of amplification and the absence of non‐specific by‐products. The PCR reactions for CsRboh genes were conducted according to Jakubowska et al ().…”

Section: Methodsmentioning

confidence: 99%

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Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H⁺‐ATPase regulation in cadmium‐stressed cucumber roots

Kabała

Zboińska

Głowiak

et al. 2018

Physiologia Plantarum

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Vacuolar H -ATPase (V-ATPase; EC 3.6.3.14) is the main enzyme responsible for generating a proton gradient across the tonoplast. Under cadmium (Cd) stress conditions, V-ATPase activity is inhibited. In the present work, hydrogen sulfide (H S) and hydrogen peroxide (H O ) cross-talk was analyzed in cucumber (Cucumis sativus L.) seedlings exposed to Cd to explain the role of both signaling molecules in the control of V-ATPase. V-ATPase activity and gene expression as well as H S and H O content and endogenous production were determined in roots of plants treated with 100 μM CdCl and different inhibitors or scavengers. It was found that H S donor improved photosynthetic parameters in Cd-stressed cucumber seedlings. Cd-induced stimulation of H S level was correlated with the increased activities of the H S-generating desulfhydrases. Increased H O and lowered H S contents in roots were able to reduce V-ATPase activities similar to Cd. H O and H S-induced modulations in V-ATPase activities were not closely related to the transcript level of encoding genes, suggesting posttranslational modifications of enzyme protein. On the other hand, exogenous H O raised H S content in root tissues independently from the desulfhydrase activity. Although treatment of control plants with H S significantly stimulated NADPH oxidase activity and gene expression, H S did not affect H O accumulation in roots exposed to Cd. The results suggest the existence of two pathways of H S generation in Cd-stressed cucumber roots. One involves desulfhydrase activity, as was previously demonstrated in different plant species. The other, the desulfhydrase-independent pathway induced by H O /NADPH oxidase, may protect V-ATPase from inhibition by Cd.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H⁺‐ATPase regulation in cadmium‐stressed cucumber roots

Kabała

Zboińska

Głowiak

et al. 2018

Physiologia Plantarum

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“…Inhibitor treatments indicated involvement of NADPH oxidases, hydroxyl radicals and CIPK upstream of a MAP kinase induction that linked to tolerance (Yeh et al, 2007). Cd stimulates expression and activity of NADPH oxidases in cucumber roots but it is not known if these responses are Ca 2+ -dependent (Jakubowska et al, 2015). It is worth noting that it is not only an increase in [Ca 2+ ] cyt that could stimulate NADPH oxidase activity but that also the restriction of Ca 2+ entry to the cytosol could cause stimulation (Mortimer et al, 2008).…”

Section: Heavy Metal Stress Has the Capacity To Distort Ca2+ Signalingmentioning

confidence: 99%

Calcium-Mediated Abiotic Stress Signaling in Roots

et al. 2016

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Roots are subjected to a range of abiotic stresses as they forage for water and nutrients. Cytosolic free calcium is a common second messenger in the signaling of abiotic stress. In addition, roots take up calcium both as a nutrient and to stimulate exocytosis in growth. For calcium to fulfill its multiple roles must require strict spatio-temporal regulation of its uptake and efflux across the plasma membrane, its buffering in the cytosol and its sequestration or release from internal stores. This prompts the question of how specificity of signaling output can be achieved against the background of calcium’s other uses. Threats to agriculture such as salinity, water availability and hypoxia are signaled through calcium. Nutrient deficiency is also emerging as a stress that is signaled through cytosolic free calcium, with progress in potassium, nitrate and boron deficiency signaling now being made. Heavy metals have the capacity to trigger or modulate root calcium signaling depending on their dose and their capacity to catalyze production of hydroxyl radicals. Mechanical stress and cold stress can both trigger an increase in root cytosolic free calcium, with the possibility of membrane deformation playing a part in initiating the calcium signal. This review addresses progress in identifying the calcium transporting proteins (particularly channels such as annexins and cyclic nucleotide-gated channels) that effect stress-induced calcium increases in roots and explores links to reactive oxygen species, lipid signaling, and the unfolded protein response.

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“…During various abiotic stresses, cellular homeostasis is altered and ROS production is elevated. Heavy metals like chromium induce oxidative stress in plant that is mediated by NADPH oxidase 10,11 . An up-regulation in heavy metal-specific, superoxide (O2• -) generating NADPH oxidase genes have also been recorded 12 .…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroids Reciprocates Heavy Metals Induced Oxidative Stress in Radish by Regulating the Expression of Key Antioxidant Enzyme Genes

Sharma

Pati

et al. 2018

Braz. arch. biol. technol.

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Modification of plasma membrane NADPH oxidase activity in cucumber seedling roots in response to cadmium stress

Cited by 59 publications

References 77 publications

Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H⁺‐ATPase regulation in cadmium‐stressed cucumber roots

Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H⁺‐ATPase regulation in cadmium‐stressed cucumber roots

Calcium-Mediated Abiotic Stress Signaling in Roots

Brassinosteroids Reciprocates Heavy Metals Induced Oxidative Stress in Radish by Regulating the Expression of Key Antioxidant Enzyme Genes

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Modification of plasma membrane NADPH oxidase activity in cucumber seedling roots in response to cadmium stress

Cited by 59 publications

References 77 publications

Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H+‐ATPase regulation in cadmium‐stressed cucumber roots

Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H+‐ATPase regulation in cadmium‐stressed cucumber roots

Calcium-Mediated Abiotic Stress Signaling in Roots

Brassinosteroids Reciprocates Heavy Metals Induced Oxidative Stress in Radish by Regulating the Expression of Key Antioxidant Enzyme Genes

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Product

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Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H⁺‐ATPase regulation in cadmium‐stressed cucumber roots

Interaction between the signaling molecules hydrogen sulfide and hydrogen peroxide and their role in vacuolar H⁺‐ATPase regulation in cadmium‐stressed cucumber roots