Modification of non-selective-solid media for aquatic bacteria

Dwinanti, Sefti Heza; Tanbiyaskur,

doi:10.19027/jai.13.163-166

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…Pure culture isolates were obtained from bacteria growing on Trypticase Soy Agar (TSA) media. TSA is a non-selective media containing agar, casein peptone, soy peptone, and sodium chloride to support the growth of various microorganisms [17]. The results of endophytic bacterial isolates formed from the 10 -1 dilution showed six bacterial colonies, and from the 10 -2 dilution, there were two bacterial colonies with different visible morphologies.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Endophytic Bacteria from Kumis Kucing Leaves (Orthosiphon aristatus Benth.)

Setiani,

Zahraeni,

Hasanah

2024

Jurnal Biotropika

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Endophytic bacteria are in symbiosis with plants and can produce secondary metabolites similar to those produced by their host. Kumis kucing (Orthosiphon aristatus) is a traditional medicinal plant that has been proven to have many pharmacological activities, including antiviral and antibacterial. This study aims to isolate and identify endophytic bacteria from kumis kucing leaves. Endophytic bacteria were isolated from kumis kucing leaf by spreading method on Trypticase Soy Agar (TSA) media and incubated at room temperature for 24 hours. Colonies that grew with bacterial morphology were inoculated on TSA media to obtain pure cultures. Pure cultures of isolates were identified through Gram staining, 16S rRNA gene sequencing, and bacterial growth curves. There were 8 isolates with bacterial morphology which identified by Gram staining. The staining results showed that all were classified as Gram negative with rod and coccus shapes. Sequencing using the 16S rRNA gene identified 3 bacterial isolates, Acinetobacter schindleri, Pantoea agglomerans, and Pseudomonas lurida. The three bacteria have different time to reach stationary phases in order to produce their secondary metabolites.

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Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Endophytic Bacteria from Kumis Kucing Leaves (Orthosiphon aristatus Benth.)

Setiani,

Zahraeni,

Hasanah

2024

Jurnal Biotropika

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“…This is because fish have a severe infection due to the attack of A. hydrophila bacteria. According to Dwinanti et al [8], an increase in the number of leukocytes indicates that the fish has a bacterial infection and the body will anticipate this condition by producing more white blood cells in response to immunity. While treatment C (KDF 0.3%) experienced a low increase of 8.97% with a leukocyte count of 11.60×10 4 cells mm 3-1 .…”

Section: White Blood Cell (Leukocyte)mentioning

confidence: 99%

Effectiveness of Potassium Diformate on Feed to Improve Immune Performance of Goldfish (Carassius auratus L.)

Yustiati

Wahyuni

Rizal

et al. 2020

AJFAR

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This research aims to determine the optimum potassium diformate (KDF) dosage which was added to feed to increase the immune status of goldfish (Carassius auratus L). This study was conducted from September 31 - November 15, 2019 at the Laboratory of Aquaculture and Molecular Biotechnology Laboratory of the Faculty of Fisheries and Marine Sciences, University of Padjadjaran. The research method used is the experimental Complete Random Design (CRD) with 4 treatments and 3 replications. The treatments are A (without KDF/control), B (0.1% KDF), C (0.3% KDF) and D (0.5% KDF). The observed parameters are survival rate, total leukocyte count (white blood cells), total erythrocyte count (red blood cells) and gross clinical sign. Observations were made after 30 days of KDF and post-test challenge by Aeromonas hydrophila for 14 days. Data on the number of total leukocyte count and total erythrocyte count were analyzed using F test and Duncan's advanced test at a 95% confidence level, while the gross clinical sign data were analyzed descriptively. The results showed that the KDF 0.3% was the optimum dose and was very effective in optimizing the performance of the goldfish immune system optimally, seen from the number of white blood cells and red blood cells which experienced the highest increase of 23.48% and 9.30%. After the challenge test, fish that were given KDF 0.3% had the highest survival rate of 46.67%. In addition, the process of recovering the number of white blood cells and red blood cells can be faster than other treatments, with a value of 12.85×104 cells mm3-1 and 0.96×106 cells mm3-1, marked also by the healing of clinical symptoms in the morphology of goldfish.

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“…Sebagai pendukung dalam proses pertumbuhannya, bakteri membutuhkan nutrisi yang berasal dari beberapa senyawa bebas di alam. Umumnya senyawa yang dimanfaatkan oleh bakteri dalam jumlah relatif besar berasal dari karbon dan nitrogen, selebihnya adalah senyawa-senyawa lain yang tersedia di alam(Dwinanti dan Tanbiyaskur, 2014). Pada lokasi penelitian, senyawa-senyawa tersebut berasal dari limbah sisa kegiatan budidaya KJA, masukan limbah domestik dari darat yang berasal dari kegiatan pertanian maupun kegiatan rumah tangga.…”

unclassified

ANALISIS KELIMPAHAN BAKTERI Pseudomonas sp. DI PERAIRAN DESA BEJALEN RAWA PENING, JAWA TENGAH

2020

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Rawa pening disebut sebagai danau alami terbentuk melalui proses letusan Gunung Api Ungaran Tua. Desa Bejalen merupakan salah satu desa di Ambarawa yang termasuk dalam lingkup wilayah Danau Rawa Pening. Karamba Jaring Apung (KJA) merupakan suatu metode budidaya dengan menggunakan jaring di permukaan perairan yang diberi pelampung. Banyak kegiatan budidaya menghasilkan limbah yang menjadi salah satu sumber bahan organik di dalam perairan. Pemecahan limbah KJA yang banyak mengandung nitrat dilakukan oleh beberapa jenis bakteri denitrifikasi secara aerob diantaranya adalah Pseudomonas sp. Tujuan dari penelitian ini yaitu untuk mengetahui kelimpahan bakteri Pseudomonas sp. di kawasan KJA dan Non KJA serta untuk mengetahui apakah terdapat perbedaan kelimpahan bakteri Pseudomonas sp. di kawasan KJA dan Non KJA. Penelitian ini dilakukan pada tanggal 15-29 April 2019 untuk pengambilan sampel air yang digunakan untuk menganalisis total bakteri Pseudomonas sp., kandungan nitrat dan oksigen terlarut (DO). Perhitungan total bakteri menggunakan metode Total Plate Count (TPC) dengan media Glutamate Starch Phenol (GSP). Total bakteri yang diperoleh di kawasan KJA lebih fluktuatif berkisar antara 1,3 x 104 hingga 8,2 x 104 CFU/ml sedangkan di kawasan Non KJA berkisar antara 0,7 x104 hingga 4,2 x104 CFU/ml. Berdasarkan hasil yang diperoleh dari uji statistik One Sample T-Test diperoleh nilai Sig. (2-tailed) terhadap data kelimpahan bakteri Pseudomonas sp. pada kawasan KJA yaitu 0,003 < 0,05 serta T hitung yang diperoleh yaitu sebesar 4,220 > dari T tabel yang bernilai 1,85955. Sementara itu, berdasarkan data kelimpahan Pseudomonas sp. di kawasan Non KJA diperoleh nilai Sig. (2-tailed) yaitu sebesar 0,001 < 0,05 serta T hitung yang diperoleh yaitu sebesar 5,070 > dari T tabel yang bernilai 1,85955. Dapat diketahui bahwa terdapat perbedaan total bakteri Pseudomonas sp. di kawasan KJA dan Non KJA.

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Modification of non-selective-solid media for aquatic bacteria

Cited by 3 publications

References 3 publications

Isolation and Identification of Endophytic Bacteria from Kumis Kucing Leaves (Orthosiphon aristatus Benth.)

Isolation and Identification of Endophytic Bacteria from Kumis Kucing Leaves (Orthosiphon aristatus Benth.)

Effectiveness of Potassium Diformate on Feed to Improve Immune Performance of Goldfish (Carassius auratus L.)

ANALISIS KELIMPAHAN BAKTERI Pseudomonas sp. DI PERAIRAN DESA BEJALEN RAWA PENING, JAWA TENGAH

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