Modification of Extracellular Vesicles by Fusion with Liposomes for the Design of Personalized Biogenic Drug Delivery Systems

Piffoux, Max; Silva, Amanda; Wilhelm, Claire; Gazeau, Florence; Tareste, David

doi:10.1021/acsnano.8b02053

Cited by 307 publications

(269 citation statements)

References 71 publications

(107 reference statements)

Supporting

Mentioning

266

Contrasting

Order By: Relevance

“…[ 60–63 ] However, current studies involving EVs delivery systems mainly focus on the synthetic hydrogels or matrix. [ 64–66 ] Notably, polymers that are not water‐soluble may not be suitable to encapsulate EVs, and the residual unreacted cross‐linkers for hydrogel making increase the potential toxicity. [ 67 ] In addition, the processing of these polymers normally involves a strong organic solvent, which may degrade the structural integrity and content of the exosomes when mixed.…”

Section: Resultsmentioning

confidence: 99%

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Wei

Shi

Zhang

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Restoring extracellular matrix (ECM) with supportive and osteoinductive abilities is of great significance for bone tissue regeneration. Current approaches involving cell-based scaffolds or nanoparticle-modified biomimic-ECM have been met with additional biosafety concerns. Herein, the natural biomineralization process is first analyzed and is found that mesenchymal stem cells-derived extracellular vesicles (EVs) from early and late stages of osteoinduction play different roles during the mineralization process. The functional EVs hierarchically with blood-derived autohydrogel (AH) are then incorporated to form an osteoinductive biomimetic extracellular matrix (BECM). The alkaline phosphatase-rich EVs are released from the outer layers to induce osteoblast differentiation during early stages. Thereafter, as the degradation of AH occurred, calcium/phosphorus (Ca/P)rich EVs are liberated to promote the nucleation of extracellular mineral crystals. Additionally, BECM contains considerable collagen fibrils that provide additional nucleation sites for crystallites deposition, thus reaching self-mineralization in situ. In conclusion, this research provides a promising, versatile mineralization-instructive platform to tackle the challenges faced in bone-tissue engineering. Scheme 1. Illustration of the BECM induced osteogenesis and biomineralization. In the early stage of mineralization, BECM secreted ALP-rich E-EVs. The E-EVs moved to intracellular mitochondria and provided ALP for phosphate metabolism, contributing to the formation of Ca/P-rich vesicles. Later, with the degradation of BECM, Ca/P-rich L-EVs were released gradually. The L-EVs aligned to extracellular collagen and promoted the nucleation of extracellular mineral crystals. Besides, L-EVs inside BECM aggregate around the internally collagen fibrils and initiated in situ mineralization. Finally, with the complete degradation of BECM, the internal mineralized components were released to the extracellular matrix, promoting the deposition formation together to realize an ideal self-mineralization process for bone tissue regeneration.www.afm-journal.de www.advancedsciencenews.com (3 of 15)

show abstract

Section: Resultsmentioning

confidence: 99%

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Wei

Shi

Zhang

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Thus, improving the EVs loading and delivering efficiency is one aspect that has been reconsidered, due to a discrepancy in the published protocol and data reproducibility [66]. Piffoux et al published a method for loading EVs using liposomes and showed impressive results [67]. However, the efficacy of this protocol has not been confirmed.…”

Section: Evs As Therapymentioning

confidence: 99%

Potential roles of extracellular vesicles in the pathophysiology, diagnosis, and treatment of autoimmune diseases

Tian¹,

Casella²,

Zhang³

et al. 2020

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Since extracellular vesicles (EVs) were discovered in 1983 in sheep reticulocytes samples, they have gradually attracted scientific attention and become a topic of great interest in the life sciences field. EVs are small membrane particles, released by virtually every cell that carries a variety of functional molecules. Their main function is to deliver messages to the surrounding area in both physiological and pathological conditions. Initially, they were thought to be either cell debris, signs of cell death, or unspecific structures. However, accumulating evidence support a theory that EVs are a universal mechanism of communication. Thanks to their biological characteristics and functions, EVs are likely to represent a promising strategy for obtaining pathogen information, identifying therapeutic targets and selecting specific biomarkers for a variety of diseases, such as autoimmune diseases. In this review, we provide a brief overview of recent progress in the study of the biology and functions of EVs. We also discuss their roles in diagnosis and therapy, with particular emphasis on autoimmune diseases.

show abstract

“…A promising compromise between EVs and liposomes was made by Piffoux et al, in 2018. [267] They fused EVs with liposomes to allow better cargo loading while preserving the natural vesicular membrane moieties, stability, and thus the EV identity. The fusion protocol was optimized by fusing 1,2-dioleoyl-snglycero-3-phospho-Lserine-N-(7-nitro-2−1,3-benzoxadiazol-4-yl) (NBD-PS) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rho-PE) lipids within the membrane of nonfluorescent EVs, resulting in increased NBD fluorescence, due to the lack of fluorescence resonance energy transfer (FRET).…”

Section: Hybrid Vesiclesmentioning

confidence: 99%

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Leggio

Arrabito

Ferrara

et al. 2020

Adv Healthcare Materials

View full text Add to dashboard Cite

Naturally occurring extracellular vesicles and artificially made vesicles represent important tools in nanomedicine for the efficient delivery of biomolecules and drugs. Since its first appearance in the literature 50 years ago, the research on vesicles is progressing at a fast pace, with the main goal of developing carriers able to protect cargoes from degradation, as well as to deliver them in a time‐ and space‐controlled fashion. While natural occurring vesicles have the advantage of being fully compatible with their host, artificial vesicles can be easily synthetized and functionalized according to the target to reach. Research is striving to merge the advantages of natural and artificial vesicles, in order to provide a new generation of highly performing vesicles, which would improve the therapeutic index of transported molecules. This progress report summarizes current manufacturing techniques used to produce both natural and artificial vesicles, exploring the promises and pitfalls of the different production processes. Finally, pros and cons of natural versus artificial vesicles are discussed and compared, with special regard toward the current applications of both kinds of vesicles in the healthcare field.

show abstract

Modification of Extracellular Vesicles by Fusion with Liposomes for the Design of Personalized Biogenic Drug Delivery Systems

Cited by 307 publications

References 71 publications

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Potential roles of extracellular vesicles in the pathophysiology, diagnosis, and treatment of autoimmune diseases

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Contact Info

Product

Resources

About