Modification of decellularized vascular scaffold with conditioned medium to enhance cell reseeding

Abstract

Repopulation of decellularized vascular scaffolds (DVS) is limited because of change in the repertoire and ratios of the remaining extracellular matrix (ECM) proteins, for example, loss of glycoproteins and the retention of type I collagen. Pre-treatment of DVS with defined ECM proteins, which match the repertoire of integrin receptors expressed by the embryonic stem cells (mESCs) to be seeded, can increase the reseeding efficacy. mESCs mainly express high levels of functional receptors for LM and FN. Reseeding efficiency of DVS with mESCs was very low, but was sigficantly increased (2.5 ± 0.1 fold) by pre-treating the DVS with A549-conditioned media. In addition, pre-treatment with A549-conditioned media led to a more homogeneous distribution of the seeded mESCs throughout the engineered blood vessel as compared to untreated DVS. This paper may promote blood vessel engineering by stressing the importance of matching the cell binding motifs of DVS and the integrin receptor repertoire of seeded cells.

Constructing heparin-modified pancreatic decellularized scaffold to improve its re-endothelialization

“…14 Pre-treatment of decellularized vascular scaffolds with defined ECM proteins, which match the repertoire of integrin receptors expressed by the embryonic stem cells to be seeded, can increase the reseeding efficacy. 15 Modifying decellularized matrix derived from porcine small intestinal submucosa (SIS) with thrombomodulin alone enables robust activated protein C generation, and thrombomodulin activity is maintained after prolonged exposure to fluid shear and blood plasma. Heparin-modified SIS has a potent anticoagulant activity.…”

Strategies for decellularization, re-cellularIzation and crosslinking in liver bioengineering

“…Pre-treating distributed switches with ECM proteins matching the integrin receptor library expressed by the seed cells can improve reimplantation. 79 A major problem in cell regeneration experiments is the black-box approach, which is the method used in most studies and the subsequent evaluation of different outcome parameters. The biomechanical aspects of the cell re-formation process have not been considered, such as whether parenchymal cells cross the vascular basement membrane during cell re-formation and how the ECM is damaged during direct injection, and the spatial and temporal resolution of the kinetics of recellularization is still poorly known.…”

“…Pre-treating distributed switches with ECM proteins matching the integrin receptor library expressed by the seed cells can improve reimplantation. 79 A major problem in cell regeneration experiments is the black-box approach, which is the method used in most studies and the subsequent evaluation of different outcome parameters.…”