Modification of cell surface glycoprotein: addition of fucosyl residues during epidermal differentiation.

Zieske, James D.; Bernstein, Isadore A.

doi:10.1083/jcb.95.2.626

Cited by 103 publications

(43 citation statements)

References 35 publications

(29 reference statements)

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“…In several differentiating systems, notably slime molds (29) and neural tissue (6), glycoproteins conferring specificity in cell recognition and adhesion have been identified. Changes in membrane glycosylation occur during terminal differentiation ofkeratinocytes, both in intact tissue (8,9,22,36) and in culture (33). Whether any of these changes affect keratinocyte adhesion and cause terminally differentiating cells to leave the basal layer is currently under investigation .…”

Section: Discussionmentioning

confidence: 99%

Selective migration of terminally differentiating cells from the basal layer of cultured human epidermis.

Watt¹

1984

The Journal of Cell Biology

180

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How terminally differentiating cells are selectively expelled from the basal layer of epidermis has been a source of interest and speculation for many years . The problem can now be studied in culture, using involucrin synthesis as an early marker of terminal differentiation in human keratinocytes . When keratinocytes are forced to grow as a monolayer by reducing the calcium ion concentration of the culture medium, they still begin to synthesize involucrin . Raising the level of calcium ions induces stratification, and cells that are synthesizing involucrin are selectively expelled from the basal layer. I have found that during calciuminduced stratification no new proteins or glycoproteins are synthesized, and the rate of cell division does not change . Movement of involucrin-positive cells out of the basal layer was found to be unaffected by cycloheximide, tunicamycin, or cytosine arabinoside . These results suggest that keratinocytes growing as a monolayer already have the necessary properties to determine their position when stratification is induced . Addition of calcium simply allows formation of desmosomes and other intimate cell contacts required for stratification . The properties of involucrin-positive cells that determine their suprabasal position include a reduced affinity for the culture substrate and preferential adhesion to other cells at the same stage of terminal differentiation . The molecular basis of these adhesive changes is discussed .

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Section: Discussionmentioning

confidence: 99%

Selective migration of terminally differentiating cells from the basal layer of cultured human epidermis.

Watt¹

1984

The Journal of Cell Biology

180

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“…Esse resultado sugere que o equilíbrio na composição química do muco que recobre a mucosa foi quebrado, passando ao possível predomínio de muco neutro. Como o muco neutro tem maior capacidade de proteção, por ser mais denso em meio aquoso, quando as células são expostas a agentes abrasivos e irritantes (BEAMISH, 1972;ZIESKE;BERNSTEIN, 1982), é possível que esse achado seja uma resposta para proteção do epitélio intestinal em razão da atrofia da mucosa. Estudos anteriores, realizados com galinhas infectadas pelo T. gondii, demonstraram resultados semelhantes com o aumento da secreção proporcional de mucinas neutras (SHIRAISHI et al, 2009;BONAPAZ et al, 2009).…”

Section: Discussionunclassified

Efeitos da infecção crônica por Toxoplasma gondii sobre a parede intestinal de gatos domésticos

Silva¹,

Araújo²,

Sant’Ana³

2010

Rev. Bras. Parasitol. Vet. (Online)

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ResumoO objetivo deste trabalho foi analisar os efeitos da infecção causada pelo Toxoplasma gondii sobre a parede do duodeno de gatos. Foram utilizados seis gatos (Felis catus), com cerca de três meses de vida, distribuídos aleatoriamente em Grupo controle (G1; n = 3) e Grupo infectado (G2; n = 3). Os animais do G2 receberam, por via oral, 200 cistos teciduais da cepa ME49 (tipo II) do T. gondii. Após 40 dias, os animais foram submetidos à eutanásia, laparotomia e retirada do duodeno, que foi fixado em solução de Bouin e submetido à rotina histológica para obtenção de cortes transversais de 3 μm. Os cortes foram corados com Hematoxilina-Eosina (HE), Azan, Ácido Periódico de Schiff (PAS), Alcian-Blue e Tricrômio de Mallory. Realizou-se uma avaliação qualitativa da parede intestinal e medidas comparativas entre os dois grupos, com relação: à espessura da túnica mucosa, túnica muscular, parede total, altura dos vilos, profundidade das criptas e altura dos enterócitos e seus núcleos. As células caliciformes, os linfócitos intraepiteliais e as células de Paneth foram quantificados. Os resultados mostraram que a infecção levou à atrofia da túnica mucosa, túnica muscular e parede intestinal do duodeno de gatos do G2 (p < 0,05). A altura dos enterócitos apresentou um aumento significativo (p < 0,05) nos animais do G2. Na avaliação qualitativa, as fibras colágenas ocupavam visivelmente uma maior área dos estratos da parede intestinal, o que sugere que estejam aumentadas. Observou-se a redução da secreção de sulfomucinas e o aumento das células de Paneth nesses mesmos animais (p < 0,05).Palavras-chaves: duodeno, toxoplasmose, epitélio intestinal, histologia, felinos. AbstractThis paper analyzes the effects of the infection caused by Toxoplasma gondii on the cat duodenal wall. Six cats (Felis catus) with 3-month-old were randomly divided into Control Group (G1; n = 3) and Infected Group (G2; n = 3). The animals from G2 received orarilly 200 T. gondii tissye cysts of ME49-strain (type II). After 40 days, the animals were submitted to euthanasia, laparotomy and had their duodenum removed, fixed in Bouin solution and submitted to histological routine obtaining 3 μm transverse cuts. The cuts were stained with Hematoxylin-Eosin (HE), Azan, Periodic acid -Schiff (PAS), Alcian-Blue, and Mallory trichrome. Qualitative assessment of the intestine wall as well as comparative measurements with respect to the thickness of mucosa, muscle tunic, total wall, the height of the villous, the depth of the crypts, and the height of the enterocytes and their nuclei were carried out. Calciform cells, the intraepithelial lymphocytes, and the Paneth cells were quantified. The results showed that the infection led to the atrophy of the mucosa, muscle tunic, and the intestinal wall of the duodenum of G2 cats (p < 0.05). The enterocytes height presented significant (p < 0.05) increase for G2 animals. According to the qualitative analysis, the collagen fibers were visibly taken a broader area on the intestinal wall layers, what suggests they have inc...

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“…We detected binding of UEA-I and SBA in cells of the epidermis, IRS, and ORS prior to terminal differentiation. As seen in epidermal cells (Brabec et al, 1980;Zieske and Bernstein, 1982;Nemanic et al, 1983;Virtanen et al, 1986;Brown et al, 1987), UEA-I binding was an earlier event than SBA binding in hair follicle cells, indicating that genes that control cell surface expression of terminal Fuc and subsequent aGalNAc are conserved in hair follicle cells. Con A and RCA-I, which have a broad specificity, also reacted with those cells regardless of their differentiation stages.…”

Section: Binding Patterns Of Con a And Rca-imentioning

confidence: 91%

“…The relationship between keratinocyte maturation and cell surface glycoconjugates has been established in the epidermis (Holt et al, 1979;Brabec et al, 1980;Reano et al, 1982;Zieske and Bernstein, 1982;Nemanic et al, 1983;Ookusa et al, 1983;Schaumburg-Lever et al, 1984;Virtanen et al, 1986, Brown et al, 1987. Binding of Ulex europeaus-I (UEA-I) and Glycine max (SBA) occurs before terminal differentiation indicating that expression of a-fucose (Fuc) and a-N-acetylgalactosamine (aGalNAc), respectively, is the specific biochemical event in epidermal keratinocytes.…”

mentioning

confidence: 98%

Glycoconjugate expression of cells of human anagen hair follicles during keratinization

1990

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Changes in the expression of glycoconjugates in cells of the inner root sheath (IRS) and outer root sheath (ORS) of human anagen hair follicles were investigated by lectin histochemistry. Concanavalin A (Con A) and Ricinus communis (RCA-I) stained hair follicle cells regardless of their differentiation stages. In IRS, Ulex europeaus-I (UEA-I) bound to the surface of the cells as soon as they were morphologically defined, and Glycine max (SBA) stained as their differentiation progressed. Innermost (IM) cells of ORS layers were reactive with UEA-I at the stage where Henle's cells were keratinized, while the reactivity of UEA-I was lost at the site of the completion of IRS keratinization where SBA reaction was detected. Staining of both UEA-I and SBA was prominent in other ORS cells at the levels where SBA binding in IM cells became strong. The staining intensity increased up to the position of the follicular isthmus. In addition, a sugar residue recognized by Dolichos biflorus (DBA) was detected in differentiated cells of ORS. In contrast, the DBA reaction was not found at all in cells of IRS, infundibulum, and epidermis. These findings identified a complexity of carbohydrate metabolism in the cells of different layers at various stages of keratinization. IM cells differentiate independently from other ORS cells but seem responsive to the degree of IRS keratinization. All ORS cells possess a unique sugar moiety not found in other keratinocytes either in the hair or epidermis.

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Modification of cell surface glycoprotein: addition of fucosyl residues during epidermal differentiation.

Cited by 103 publications

References 35 publications

Selective migration of terminally differentiating cells from the basal layer of cultured human epidermis.

Selective migration of terminally differentiating cells from the basal layer of cultured human epidermis.

Efeitos da infecção crônica por Toxoplasma gondii sobre a parede intestinal de gatos domésticos

Glycoconjugate expression of cells of human anagen hair follicles during keratinization

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