Modification of Biological Activity of Lipopolysaccharide in the Complex with Chitosan

Ermak, I. M.; Давыдова, В. Н.; Горбач, В. И.; Berdyshev, E. L.; Кузнецова, Т. А.; Ivanushko, I. A.; Гажа, А. К.; Smolina, T P; Ts, Zaporozhets; Соловьева, Т. Ф.

doi:10.1023/b:bebm.0000035136.61014.c2

Cited by 9 publications

(6 citation statements)

References 7 publications

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“…Since chitosan is a chitin derivative, there is the possibility of endotoxin presence, which could result in inflammation [13]. There is information on the anti-inflammatory effect of the complex of chitosan and lipopolysaccharide, which is the most common endotoxin, [14] as well as about its partial dissociation [15] on interaction with chitosan. Moreover, the treatment of endotoxins with acids and alkalis (more than 0.1 M) results in partial destruction [16].…”

Section: Introductionmentioning

confidence: 99%

Hemocompatible Chitin-Chitosan Composite Fibers

et al. 2020

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Composite chitosan fibers filled with chitin nanofibrils (CNF) were obtained by the wet spinning method. The paper discusses the mechanical properties of such type fibers and their hemocompatibility, as well as the possibility of optimizing these properties by adding chitin nanofibrils. It was shown that low CNF concentration (about 0.5%) leads to an increase in fiber tensile strength due to the additional orientation of chitosan macromolecules. At the same time, with an increase in the content of CNF, the stability of the mechanical properties of composite fibers in a humid medium increases. All chitosan fibers, except 0.5% CNF, showed good hemocompatibility, even on prolonged contact with human blood. The addition of chitin nanofibers leads to decrease in hemoglobin molecules sorption due to the decline in optical density at wavelengths of 414 nm and 540 nm. Nevertheless, the hemolysis of fibers was comparable or even lesser that carbon hemosorbent, which is actively used in clinical practice.

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Section: Introductionmentioning

confidence: 99%

Hemocompatible Chitin-Chitosan Composite Fibers

et al. 2020

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“…Such cationic compounds as lysozyme [8] and polymyxin [7] neutralize endotoxins by forming macromolecular complexes with them. We previously showed that chitosan, a natural polysaccharide, forms stable complexes with endotoxins, thus appreciably reducing their toxicity [1] and modifying biological properties of LPS [2]. Here we studied the possibility of using polysaccharide carrageenan for this purpose.…”

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confidence: 99%

Natural polysaccharide carrageenan inhibits toxic effect of gram-negative bacterial endotoxins

et al. 2006

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The protective effect of polysaccharide carrageenan on the damaging effect of endotoxins of gram-negative bacteria was studied in vivo and in vitro. Carrageenan increased mouse resistance to the toxic effect of LPS. The degree of protection depended on polysaccharide concentration and administration time and route. Pretreatment of donor platelets with carrageenan reduced their aggregation activity caused by cooperative effect of LPS and ADP.

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“…Second, the complex reaction of LPS and CMC may reduce the inflammatory response. In fact, a previous study showed that LPS has an anion and CMC has a cation; thus, when the two substances react, they form a complex (5,7). This suggests that the inflammatory response to LPS is reduced, and therefore, the reaction to chitosan cannot be clearly observed.…”

Section: Wwwksvcorkrmentioning

confidence: 96%

“…To the best of our knowledge, the effects of CMC on the proliferation and migration of canine BMSCs in vitro have been reported here for the first time. LPSs are a major component of cell walls in gram-negative bacteria; they modulate the activities of most systems in infected microorganisms, inducing various pathophysiological changes (7). The molecules of LPS exhibit a variety of biological activities that may be beneficial at low concentrations but may be endotoxic at higher concentrations due to an overproduction of cytokines by immune cells, such as TNF-α and ILs (5).…”

Section: Wwwksvcorkrmentioning

confidence: 99%

Carboxymethyl Chitosan Promotes Migration and Inhibits Lipopolysaccharide-Induced Inflammatory Response in Canine Bone Marrow-Derived Mesenchymal Stem Cells

et al. 2021

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The study was conducted to evaluate the effects of carboxymethyl chitosan (CMC) on proliferation, migration, and lipopolysaccharide (LPS)-induced inflammatory response in canine bone marrow-derived mesenchymal stem cells (BMSCs). The proliferation and migration of BMSCs were examined after treatment with CMC. The effect of CMC on the mRNA expression of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, IL-10, and transforming growth factor (TGF)-β, was also evaluated by reverse transcription polymerase chain reaction (RT-PCR). In the proliferation assay, no significant changes were found at all CMC concentrations compared with controls. The migration assay showed that CMC dose-dependently stimulated the migration of BMSCs in normal and LPS-treated conditions. RT-PCR showed that TNF-α and IL-10 expressions were suppressed in the BMSCs after CMC treatment. However, other genes were not affected. Taken together, CMC promoted BMSC migration and inhibited TNF-α and IL-10. Therefore, CMC may be possible to regulate wound healing when mesenchymal stem cells are applied in inflammatory diseases.

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Modification of Biological Activity of Lipopolysaccharide in the Complex with Chitosan

Abstract: In the complex with chitosan, lipopolysaccharide partially lost its ability to induce lymphokines tumor necrosis factor and interleukin-8, but retained immunostimulating properties and increased phagocytic function of macrophages by improving digestion of bacteria.

Cited by 9 publications

References 7 publications

Hemocompatible Chitin-Chitosan Composite Fibers

Hemocompatible Chitin-Chitosan Composite Fibers

Natural polysaccharide carrageenan inhibits toxic effect of gram-negative bacterial endotoxins

Carboxymethyl Chitosan Promotes Migration and Inhibits Lipopolysaccharide-Induced Inflammatory Response in Canine Bone Marrow-Derived Mesenchymal Stem Cells

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